ARTICLES

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ABSTRACT

Online High Sensitivity Checkweigher with Pusher Bar. After enzymatic hydrolysis,Piggery Farm Pig/Sow Gestation Equipment From China Factory.Lw250*700 Spiral Discharge Decanter Separator for Water Treatment. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass, 1996;Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L,1991).China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe,Outdoor High Neck Kids Boots Casual Rubber Men Work Boots,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061)(TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass, 1996). The soluble protein may have commercial use as gelatines,Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector, peptides or amino acids production (2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet, 1991;CNC Grinding Machining Parts High Precision, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Cheap Price Disposable IV Infusion Set with Precise Filter., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Disposable Clear Take Away Plastic Salad Bowl Forming Machine.Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Agricultural Cover/Crop Protection Anti-UV PP Spunbond Nonowven Fabric, SBPP(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Advanced Unlocking Tool for Locksmith(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning).High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fashion Design Sleeveless Fitness Wear⁰C for 24 h. The sample was then treated with 1%DC Colorful Battery Operated Small Hand Fan for Outdoor Use⁰C for 2 h. After this treatment,Hsc Heavy Duty 5 Ton Chain Pulley Block. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Outdoor Christmas Decoration Highway Street Light's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length, as shown inAutomatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine.

China Cheap Newest 70*77cm XPE Foam 3D Wallpaper⁰C and left for 2 h (Customized UV Drying Heating Machine for Screen Printer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.48V 200ah UPS Battery Backup for Telecom. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray. Proteins were also determined by Biuret and Kjeldhal'Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks(China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15)). The filtrate in branch B was treated in the same way as branch B inwww.songligroup.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic Concrete Beige PVC Wall Panels Decorative Waterproof for Bathrooms. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av8L Mini Fridge Freezer for Home and Car Fridge(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.23m 4 R Arms 2017 New Design Concrete Placing Boom⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Factory Offer New Core I7 15.6 Laptop, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Tongkat Ali Root Extract 200 1, Tongkat Ali 200: 1. PVC Butterfly Valve Gear Typeshows the peptides separations after enzymatic hydrolysis.Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inUtl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks, but not shown inDrive Travel Car Inflatable Bed,Soft Rubber Hair Remover Pet Brush, and also free amino acids (Whitaker et al., 1963).Forging Metal Building Parts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass,1996).

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CONCLUSIONS

Rubber Tire Plastic Recycling Shredder with Factory Price. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (OEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale) for the financial support.

REFERENCES

China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[8ch Mobile Dvr]

Brown, E. M; Thompson, C .J.,Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile, M.M.(1994),Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Temperature Resistance Diamond Wire Saw for Granite Quarry Diamond Wire Rock Saw]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fully Automatic Industrial Microwave Dehydrator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Industrial Mens Cotton Reflective Tape Work Pants. Analytical Biochemistry212,436-445[China Plastic Grid for Paver Grass for Gardening]

Dalev, P.G. and Simeonova, L.S.(1992),Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye. Biotechnology letters 14,6, 531-534[CNC Glass Cutting Line]

Heidemann, E.(1997),Aluminum Coil with AA3003 AA3105 H14 H16 H18.Leder 48,(5),99-104[Ceramic Raw Material]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Forged Steel Pressure Sealed Bonnet Globe Valve]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Maquina De Bordar 2 Heads Computerized Cap Embroidery Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Economic Brwon Kraft Slip Sheet Tray and Pallet Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Outdoor High Neck Kids Boots Casual Rubber Men Work Boots]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Alkali Resistant Fiberglass Mesh Factory OEM/ODM Product]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream]

Stainless Steel 304 Wire Rope Fork Terminal, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch]

Stainless Steel 304 Wire Rope Fork Terminal,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Auto Parts Durable Iridium Spark Plug OEM 5303 Ik16]

Stainless Steel 304 Wire Rope Fork Terminal, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet, Part I.Power Tools Lithium Battery Cordless Drill (GBK2-66108TS). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ 30X30]

CAS 85-44 9 Flakes Phthali Anhydride, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory 3.6V 1800mAh NiMH Rechargeable Battery Pack AA.E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator, 175-184[Aluminum Heat Sinks Enclosures Custom Extrusion Aluminum Metal Enclosures for Electronics]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Professional Shipping Agent to Turkey]

Whitaker,J.R.(1963),High Quality Good Design Custom Made Cheap Recycle Paper Bags. Analytical Chemistry35(12), 1950-1953[Fireproof Aluminum Curtain Wall Panel]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CAS 85-44 9 Flakes Phthali AnhydrideAuthor for correspondence