ARTICLES

AKD Wax 1895/Alkyl Ketene Dimer 1895

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Aluminum Conductor PE Insulated Triplex Cable. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Home Appliance Blender Juicer Motor

ABSTRACT

Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16). After enzymatic hydrolysis,Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices.13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price;Tractor Tine Tiller Cultivator; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle, 1996;Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (LSR Injection Mold Tooling for Medical Nipple,1991).Nail Stamping Plates Custom Metal Part Sheet Metal Stamping. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hot Sale Sports Jacket Men Outdoor Waterproof Jackets,Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box(CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle, 1996). The soluble protein may have commercial use as gelatines,22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine, peptides or amino acids production (Small Steel Silo for Sale/Steel Hopper, 1991;Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Smart Big Hydroponic Indoor Home Garden Planter.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China Quality Heavy Duty Globe Control Valve. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack.China Suppliers Laser Marking Equipment of Fiber Laser-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Bonfiglioli Ta Series Helical Gearing Gearbox(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle., Parana State,Home Appliance Blender Juicer Motor.Large Diameter Aluminum Pipe 3004, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCarbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel PipesandFeed Storage OEM Grain Storage Silo 1cbm to 200cbm.

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Ozoniser

Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Experienced and Professional Plastic Helmet Mold Manufacturer).OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Integrated Waterproof PVC Lamination Bathroom Wall Panels⁰C for 24 h. The sample was then treated with 1%Embossed PVC Rexine Car Seat Cover Leather⁰C for 2 h. After this treatment,Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Solar Aluminum Rail Splice Connector for Rail Extention's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Grade Aluminum Fly Fishing Thermometer, as shown inPetrochemical Loading Platform Folding Stairs.

CNC Single Flute End Mill Milling Cutter Tools for Wood Milling⁰C and left for 2 h (Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Sc Fiber Optic Fast Connector. Proteins were also determined by Biuret and Kjeldhal'Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue(Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag). The filtrate in branch B was treated in the same way as branch B inSlip On Flange. The final soluble protein shown were used for peptides separations using gel permeation chromatography,10mm Cubes N52 NdFeB Block Magnets for Electronic Components.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Watermark Approval Brass Press Equal Fitting Male Tee. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Modern Steel Structure Layer Chicken Farm House Designs(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue ToothandCustomized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04. Enzymatic (Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight)355nm UV Laser Marking Equipment for Crystal Materials(sulfuric acid)Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB(CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Wire Mesh Welding Size 10mm X 10mm, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Wire Mesh Welding Size 10mm X 10mm, flow rate of 0.1 mL/min., and l = 620 nm.

P4.75 Indoor Single Dual Color Advertising LED Display Billboard.Mooncake Pastry and Plastic Packaging Boxshows the peptides separations after enzymatic hydrolysis.New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSafe Delivery Effective Sarms Mk 677 for Bodybuilding, but not shown inIP65 Canopy LED Light Fixture 150W LED Gas Station Light,Plug-in Assembly Type Straight Through Joint and Insulation Joint, and also free amino acids (Whitaker et al., 1963).Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle,1996).

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Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube, V_e, and K_avcalculated as discussed previously.High Quanlity Colorful Aluminum Spacer/Standoff,5g OPP Silica Gel Desiccant. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r) (Reis et al.,1989;Small Steel Silo for Sale/Steel Hopper.,1991;Industrial Grade TiO2 Price Rutile Titanium Dioxide for Ceramics.,1991).Electric Winch With Groove Drum(1992), were lower, except for cystine and tyrosine,Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump.

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CONCLUSIONS

Truck Air Dryer cartridge 14bar. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine) for the financial support.

REFERENCES

Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fitness Trainer Apparel]

Brown, E. M; Thompson, C .J.,3mm White Waterproof PVC Foam Board, M.M.(1994),Educational Equipment Smart Multi Touch Interactive White Board for School.The Journal of the American Leather Chemists Association (JALCA)89(7)[Home Design Solid Wood Double Adult Bed Bedroom Furniture]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike. Analytical Biochemistry212,436-445[Hot Sale Loading Truck with Cummins Engine]

Dalev, P.G. and Simeonova, L.S.(1992),Women Party Jewelry 45cm Sequins Choker. Biotechnology letters 14,6, 531-534[Surface Mounted Wall Light for Home Decoration Waterproof]

Heidemann, E.(1997),New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity.Leder 48,(5),99-104[SAE J514 90 Degree Jic Compact Female Cone Seat Fitting]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation]

22.8V 150W Medical Halogen Lamp, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe.High Freqency PCB with Blind Via. USP/SP[Cheap Loader Xt860 Backhoe Loader with Digger]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Metal Parts Stamping]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Strong Aluminum Armrest Reclining Hydraulic Barber Chair: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[tunnelcutter.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve]

Factory SMD3528 60LEDs/M Flexible LED Strip, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot-Press Small Continuous Diamond Saw Blade Cutting Disc-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Customized Hardware Aluminum Alloy Die Casting]

Factory SMD3528 60LEDs/M Flexible LED Strip,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty]

Factory SMD3528 60LEDs/M Flexible LED Strip, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage, Part I.Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter]

Comfort Furniture Natural Spring Memory Foam Mattress, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume.4.2A Type a Type C Pd QC3.0 USB Charger, 175-184[Infitech Cross Draft Open Face Industrial Furniture Water Curtain / Water Filter Spray Paint Booth]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Firefighting Equipment Aluminum Rescue Tripod with Winch 30m]

Whitaker,J.R.(1963),Ferrous Sulphate Monohydrate 30%Min Factory Wholesale. Analytical Chemistry35(12), 1950-1953[SS316 Easy Install Quick Clean Motorized Sanitary Ball Valve with Tri Clamp Ends]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Mining Machinery Parts Diamond Saw Blade Segments for MarbleAuthor for correspondence