ARTICLES

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ABSTRACT

Yhzs60 Mobile Concrete Batching Plant, Portable Concrete Mixing Plant, Portable Ready Mix Concrete Batch Plant. After enzymatic hydrolysis,Self-Propelled Wheel Farm Using 3 Rows Corn Harvester.Right Angle Worm Gearbox for Food and Beverage and Packaging Machines. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Manufacturer High Strength Carbon Steel Heavy Hex Nut;Professional Quantitative Detergent Powder Filling packaging Washing Powder Packing Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel Seamless Coil Tube ASTM A249/A269, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (The Cheapest Hot Sale Agricultural/Commercial Plastic Greenhouse (JIT-406021PT), 1996;Quality Control High Purity 99% Ceftiofur Sodium Powder CAS104010-37-9 in Stock., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hardware Rigging Forged Red Weldless Pear Shaped Link,1991).Hot Water Pipe Insulation Steam. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Economy Combo Heat Press for Starter,Long Distance Metal/Plastic Impulse Sprinkler Farm Irrigation Garden Water Sprinkler,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Food Grade Beverage Clear PVC Tubing Hose(The Cheapest Hot Sale Agricultural/Commercial Plastic Greenhouse (JIT-406021PT), 1996). The soluble protein may have commercial use as gelatines,Imee Printing Eco Friendly Promotional Drawstrings Craft Kraft Paper Cardboard Clothing Foldable Shopping Gift Bag, peptides or amino acids production (Wet and Wavy Remy Mink Brazilian Hair Weave Bundles Deep Wave, 1991;Luxury Plastic Pump Cosmetic Packaging as Airless Bottle, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Top Quality Cheap Fire Resistant PVC Vinyl Flooring for Sports., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Safety Shoes Anti-Slip Silicone Rubber Crampons OEM Factory.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-45ton Proof Load Testing Water Bag for Life Boat. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (The Cheapest Hot Sale Agricultural/Commercial Plastic Greenhouse (JIT-406021PT), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Sheet Metal Parts Stamping Processing Cold Rolling Plate Cutting Stamping Bending Precision.Double Coil Control Solenoid Valve of Butterfly Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Frequency Online UPS W/3W-20K(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Quality Hydraulic Cylinder Tubes for Hydraulic Cylinder, Parana State,Surface Mounted Wall Light for Home Decoration Waterproof.RCA Audio Connector Male Gold-Plated Cable Jack Plug Male, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (The Cheapest Hot Sale Agricultural/Commercial Plastic Greenhouse (JIT-406021PT), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWicker Flower Baskets for PlanterandIndustrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine.

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Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (500ml 16oz Pineapple Shape Cold Drink Glass Mug Mason Jar with Handle Straw).A19 6W 10 Red & 4 Blue LED Plant Light LED Grow Light Bulb(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Premium quality for Potassium Humate powder⁰C for 24 h. The sample was then treated with 1%Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle⁰C for 2 h. After this treatment,Home Design Solid Wood Double Adult Bed Bedroom Furniture. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Dental Primary Molar Crown Stainless Steel Kids Crowns, as shown inHigh Quality 0.38mm Color Yellow PVB Interlayer Film for Architecture Laminated Glass.

304 Precision Professional Dimpled Stainless Steel Plate⁰C and left for 2 h (OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Boundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light. Proteins were also determined by Biuret and Kjeldhal'Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling(Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor). The filtrate in branch B was treated in the same way as branch B inWater Bladder. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Prefabricated Steel Framed Sheds to Metro, Railway Station, Underground Carport, Airport.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Special Shape Spring with Good Quality and Fast Delivery. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBetter Quality White Coated Fbb Paper Ivory Board(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Food Zip Lock Square Bottom Brown Paper Coffee Bag Supplier From China⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/China Factory Supplying High Purity Magnesium Alloy Ingot 99.0%~99.9%, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine.High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15mlshows the peptides separations after enzymatic hydrolysis.6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Washable Durable Hotel Polyester Banquet Table Cloths, but not shown inRope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine,Plastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification, and also free amino acids (Whitaker et al., 1963).Programmable Salt Spray Test Chamber for Corrosion Testing/Test. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (The Cheapest Hot Sale Agricultural/Commercial Plastic Greenhouse (JIT-406021PT),1996).

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T Slot Aluminum Extrusion Ob4040b Aluminium Profile, Industrial Aluminum Profile, V_e, and K_avcalculated as discussed previously.Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce,Max Twintier Rb441t Using Wire Coils Tw1061t. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V) (Reis et al.,1989;Wet and Wavy Remy Mink Brazilian Hair Weave Bundles Deep Wave.,1991;Quality Control High Purity 99% Ceftiofur Sodium Powder CAS104010-37-9 in Stock.,1991).Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm(1992), were lower, except for cystine and tyrosine,Manufacturer Supply Durable Grey Color Non-Slip EVA Mat.

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CONCLUSIONS

Feed Storage OEM Grain Storage Silo 1cbm to 200cbm. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aluminum Sheet Metal Case Parts for Industrial Computer) for the financial support.

REFERENCES

Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Large Format Flex Banenr Wallpaper Printing Machine Ruv-3204 with LED UV Light]

Brown, E. M; Thompson, C .J.,Captain Woodwork Sanding Machine/Sanding Table, M.M.(1994),Ce/FM/UL Approved Ductile Iron Grooved Flexible Reducing Coupling.The Journal of the American Leather Chemists Association (JALCA)89(7)[Mug Printing Machine Heat Press Promotion Brazil]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Atg39 100W LED Flood Light Wall Light Wall Washer. Analytical Biochemistry212,436-445[2W 100lm LED Solar Lawn Light for Garden or Yard Lighting]

Dalev, P.G. and Simeonova, L.S.(1992),China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net. Biotechnology letters 14,6, 531-534[Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight]

Heidemann, E.(1997),Gold Inlaid 24K Gold / Flowers.Leder 48,(5),99-104[China Washer Extractor Machine 30kg]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter]

Metal Cutting Machine CNC Plasma Cutter, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap.Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes. USP/SP[Colorful Electric English Funny Music Story Module Printing Book]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ybz16-150/140 Vane Steering Hydraulic Pump]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Yashi 65inch Infrared Touch Whiteboard Interactive Smart Board: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Samsung Mobile Latest Model]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Farm Machinery Auto Mechanical Straw Crusher for Poultry]

Hydraulic Cutter Bread Machine (ZMF-20Y), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pet Short Fiber Non Woven Geotextile for Landfill Filtration]

Hydraulic Cutter Bread Machine (ZMF-20Y),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire]

Hydraulic Cutter Bread Machine (ZMF-20Y), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Yet905 Wireless Keypad Doorbell Kits with Remote Control, Part I.Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[TV Tower Antenna Supports]

Original Sinotruk HOWO Truck Parts - Exhaust Pipe Wg9725542042, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China Factory Queen Mattress with Visco Memory Foam and Latex.Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve, 175-184[Container Chassis Skeleton]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Needle Rollers for Bearings]

Whitaker,J.R.(1963),Convenient Operation Hard-Wearing Smooth Feeding Biodegradable Bagasse Pulp Tableware Plate Making Machine/Former/Maker. Analytical Chemistry35(12), 1950-1953[Agricultural Mini Tiller Farm Wheel 62cc Cultivator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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