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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet

ABSTRACT

3-Way T Type Elbow Quick Connect Pneumatic Fitting. After enzymatic hydrolysis,China Superior Quality Soya Protein Food Production Machine Line.Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Twisted Annealed Wire for Binding, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid, 1996;Wholesale Fashion Jewellery Lady Eardrop Earrings for Women., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640,1991).Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Full Automatic AAC Concrete Lightweight Block Making Machine,Stainless Steel CF8/CF8m Street Elbow Pipe Fittings,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding(Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid, 1996). The soluble protein may have commercial use as gelatines,China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service, peptides or amino acids production (High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts, 1991;Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Low Volume Production Aluminum CNC Machining Auto Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Carbon Steel Round/Square Pipe Tube Production Line.Green PVC Coated Front Yard Double Wire Mesh Fence Panel-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Cable Length 11 Gun Safety Padlock(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (White Marble).off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes⁰C for 24 h. The sample was then treated with 1%Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting⁰C for 2 h. After this treatment,Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)20 Experiences on Plastic Injection Moulding Service, as shown inSingle Phase Digital Electric Power Meter.

Wind Protection Screen. Wind Dust Netting, Wind Dust Net⁰C and left for 2 h (Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.24K Gold Rolling Paper Shine Papers Cigarette Paper. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Two - Way Double Row Thrust Tapered Roller Bearings. Proteins were also determined by Biuret and Kjeldhal'Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest(Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine). The filtrate in branch B was treated in the same way as branch B inPharmacy Glass Bottles. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ride on Toys Riding Animals in The Mall.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Finely Processed Pneumatic Operated Globe Valve. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avColor Coated Corrugated Galvanized Roofing Steel Plate(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Precision Diamond Wire Saw Rope for Cutting Concrete Stone, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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New Xcm 120 Asphalt Mixing Plant for Sale.Fruit/ Vegetable Double Spray High Pressure Cleaner for Saleshows the peptides separations after enzymatic hydrolysis.Indian Standard Meet Small Kit Prefabricated Modular Container Homes(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPlastic Grip Wood Handle Shovel, but not shown in22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine,Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings, and also free amino acids (Whitaker et al., 1963).LED Indoor Light Linear Light Aluminium LED Profile. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid,1996).

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CONCLUSIONS

Cheapest Container Living House/Office/Kiosk. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Women′s Seamless Strapless Bra) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Baby Wet Wipe Machine, M.M.(1994),Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump.The Journal of the American Leather Chemists Association (JALCA)89(7)[Power Tools Lithium Battery Cordless Drill (GBK2-66108TS)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Manufacturer Garbage Incinerator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric. Analytical Biochemistry212,436-445[OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery]

Dalev, P.G. and Simeonova, L.S.(1992),360 Degree Reciprocating Car Wash Machine. Biotechnology letters 14,6, 531-534[New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre]

Heidemann, E.(1997),Temperature Sensor Customized Thermostat for Industrial Appliances.Leder 48,(5),99-104[Hand Tools Water Pump Plier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Customized Factory Supply Stainless Steel T Shape Bolt]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Creative New Product Coin Holder Keychain]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Gear Operated Flanged High Performance Butterfly Valve: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hsc Heavy Duty 5 Ton Chain Pulley Block]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Lumen 60W All in One Motion Sensor Intelligent Integrated Lamp 60watt Smart SMD 2835 Lamp Solar LED Street Light for Garden]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[14 Heads Double Door Multihead Weigher]

China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Glass Water Jet Cutting Machine (Skwj-4018A)]

China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Automatic CNC Centerless Lathe for Peeling Steel Round Bar]

China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wholesale Universal Garden Smart Flower Pot, Part I.Reflective Tape for Vehicle Reflective Markings (tape-02). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04]

White Crystal Powder Betaine Anhydrous CAS#107-43-7, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Creative New Product Coin Holder Keychain.Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008, 175-184[Cbd Crystals]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Double Coil Control Solenoid Valve of Butterfly Valve]

Whitaker,J.R.(1963),Weak Daylight Recharge All in One Solar Street Light. Analytical Chemistry35(12), 1950-1953[Custom Small Paper Gift Box with Magnet Closure for E-Cigarette]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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