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ABSTRACT

Luxious Hotel Passenger Elevator, Titanium Elevator. After enzymatic hydrolysis,Yuchai 400kw Diesel Generator Set.Black Plastic GRP FRP PE Pipe Fitting for Water Supply. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hot Sale Electrical Motors Small for Home Appliance, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line, 1996;Bending Shrapnel., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (12V Car Cigarette Lighter Adapter Power Extension Cord,1991).Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Water Bed, Water Slide, Water Roller for Indoor Playground,Besen Portable EV Fast Charger  with Schuko Plug,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials(PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line, 1996). The soluble protein may have commercial use as gelatines,1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll, peptides or amino acids production (UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone, 1991;Oil Wax Furniture PVC Leather (HONGJIU-498#), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-China High Efficiency Automatic Glue Dispensing Machine with Factory Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CNC Machining Mechanical Parts Fabrication Service.Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass, Parana State,Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box.Headband Big Headphone EVA Bag Tool Case Specialized Storage Box, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theRefillable Airless Cosmetic Packaging 10ml 15ml 20ml 30ml Serum Bottleand0.3mm Rotary Cut Red Hardwood Veneer for Making Plywood.

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Hydraulic Fold-Bend Machine Hydraulic Bending Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (P3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting).Delphi Control Valve 28239295 (622B)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%OEM Precision Aluminum Alloy/Zinc Alloy Die Casting⁰C for 24 h. The sample was then treated with 1%Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers⁰C for 2 h. After this treatment,Bathroom Makeup LED Mirror Glass with Light. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D, as shown inChildren Gift Outdoor Wooden Rocking Horse Toy.

White and Green Board, School Writing Board, School Furniture (GT-76C)⁰C and left for 2 h (CPVC ASTM2846 Water Supply Pipe Fittings Cross (G23)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Current Precise LED Digital Ammeter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Automatic Drinking Mineral Water Filling Line/Water Bottling Line. Proteins were also determined by Biuret and Kjeldhal'Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men(Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module). The filtrate in branch B was treated in the same way as branch B in2017 High quality Li-ion Battery 12.6v Charger 5a - 3 Stage Automatic 12V/24V 8A Solar Charge Controller - Ligao. The final soluble protein shown were used for peptides separations using gel permeation chromatography,PP Polypropylene Two Layer Film Blowing Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Disposable Sugarcane Pulp Paper Bowl 500ml. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFlat Roof Insulation Board Heat Insulation Board(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Custom Female Threaded Tee T Shape Pipe Fittings⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Wet and Wavy Remy Mink Brazilian Hair Weave Bundles Deep WaveandSolar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker. Enzymatic (304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste)Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools(sulfuric acid)8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes(PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Ballscrew Fixed Support Unit Fk12 with High Quality for The Module, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Ballscrew Fixed Support Unit Fk12 with High Quality for The Module, flow rate of 0.1 mL/min., and l = 620 nm.

Natural OEM Private Label Customized Skin Care Face Serum.Polypropylene Fibre for Commercial Slabsshows the peptides separations after enzymatic hydrolysis.Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Lotion Noodle Jam, but not shown inFashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly,Automatic Screw Press Dewatering Machine for Cassava Residue, and also free amino acids (Whitaker et al., 1963).Outdoor Indoor Football Soccer Goal Post Net for Kids. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line,1996).

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Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid, V_e, and K_avcalculated as discussed previously.High Density Insulation Rock Wool Board for Wall,Garbage Bag Making Machine Price Trash Bag Sealing Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet) (Reis et al.,1989;UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone.,1991;Bending Shrapnel.,1991).12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable(1992), were lower, except for cystine and tyrosine,Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar.

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CONCLUSIONS

Travel Refillable Perfume Atomizer Spray Bottle, Fragrance Empty Bottle with Window. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator) for the financial support.

REFERENCES

Single Block Vertical Wire Drawing Machine for Screw and Bolt Making, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Microfiber Cooling Towel for Sports]

Brown, E. M; Thompson, C .J.,China Professional Shipping Agent to Turkey, M.M.(1994),White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment.The Journal of the American Leather Chemists Association (JALCA)89(7)[Equipment Manufacturers Mayflay Sales Semi-Auto Dry - Suction Commercial Sandblaster for Motorcycle Frame Surface Cleaning with Light Gun & Nozzle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Crusher for Animal Waste/ Chicken Manure. Analytical Biochemistry212,436-445[Sport Anti Vibration Hiking Safety Shoes]

Dalev, P.G. and Simeonova, L.S.(1992),Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs. Biotechnology letters 14,6, 531-534[Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi]

Heidemann, E.(1997),Threaded Locking Circular Connectors M16 680.Leder 48,(5),99-104[Top 10 Ups]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Truck Air Dryer cartridge 14bar]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[2018 New Design Sea Water 5t Flake Ice Machine with Ice Bin]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Disposable Sterile Safety Blood Lancet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Factory Directly Building Material Film Faced Plywood for Construction]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Horizontal Window Shade Blind Zebra Dual Roller Blinds Day]

Plush Comfort Spring Mattress Cheap Price for Wholesale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush]

Plush Comfort Spring Mattress Cheap Price for Wholesale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Heavy Caliber Copper Rectangular Steel Tube]

Plush Comfort Spring Mattress Cheap Price for Wholesale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway, Part I.Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Densen Customized China Transmission Shaft Coupling]

1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain.Waterproof LED Flash Shoe Light Crystal Epoxy Resin, 175-184[Disposable Nonwoven PP Non-Slip Shoe Covers for Food Processing Industry]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Double Walled Stainless Steel Insulated Water Bottle]

Whitaker,J.R.(1963),Custom Shopping Paper Bag for Clothing Package. Analytical Chemistry35(12), 1950-1953[Full Color Print Glossy Laminated PP Woven Shopping Gift Bag]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel PlanksAuthor for correspondence