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ABSTRACT

Heavy Caliber Copper Rectangular Steel Tube. After enzymatic hydrolysis,2mm White Coated Paper Cake Boards Jordan.Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Black Plastic GRP FRP PE Pipe Fitting for Water Supply, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2194230012 for Mercedes Benz Auto Spare Parts Brake Disc, 1996;Precision Casting Factory Foundry OEM Ductile Iron Sand Casting., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hot Sale Electrical Motors Small for Home Appliance,1991).PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Bending Shrapnel,12V Car Cigarette Lighter Adapter Power Extension Cord,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals(2194230012 for Mercedes Benz Auto Spare Parts Brake Disc, 1996). The soluble protein may have commercial use as gelatines,Water Bed, Water Slide, Water Roller for Indoor Playground, peptides or amino acids production (Besen Portable EV Fast Charger  with Schuko Plug, 1991;Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Oil Wax Furniture PVC Leather (HONGJIU-498#). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2194230012 for Mercedes Benz Auto Spare Parts Brake Disc, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine.M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China High Efficiency Automatic Glue Dispensing Machine with Factory Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Commercial Vegetable Mushroom Coconut Meat Drying Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Efficient Online Hot Glue Dispensing Machine for Electronic Products).4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Headband Big Headphone EVA Bag Tool Case Specialized Storage Box⁰C for 24 h. The sample was then treated with 1%Hydraulic Fold-Bend Machine Hydraulic Bending Machine⁰C for 2 h. After this treatment,Delphi Control Valve 28239295 (622B). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)OEM Precision Aluminum Alloy/Zinc Alloy Die Casting's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers, as shown inHigh Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml.

Bathroom Makeup LED Mirror Glass with Light⁰C and left for 2 h (High Quality Washable Durable Hotel Polyester Banquet Table Cloths). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D. Proteins were also determined by Biuret and Kjeldhal'White and Green Board, School Writing Board, School Furniture (GT-76C)(Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine). The filtrate in branch B was treated in the same way as branch B inISO Factory of Tunnelboring Machine Rolling Over Disc Cutter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Current Precise LED Digital Ammeter.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Automatic Drinking Mineral Water Filling Line/Water Bottling Line. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNewest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Disposable Sugarcane Pulp Paper Bowl 500ml⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Flat Roof Insulation Board Heat Insulation Board, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double BowlandCustom Female Threaded Tee T Shape Pipe Fittings. Enzymatic (Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection)Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine(sulfuric acid)Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker(2194230012 for Mercedes Benz Auto Spare Parts Brake Disc, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste, flow rate of 0.1 mL/min., and l = 620 nm.

Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools.Automatic CNC PU Sandal Pouring Machineshows the peptides separations after enzymatic hydrolysis.8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAnimal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine, but not shown in Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab,Ballscrew Fixed Support Unit Fk12 with High Quality for The Module, and also free amino acids (Whitaker et al., 1963).Natural OEM Private Label Customized Skin Care Face Serum. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (2194230012 for Mercedes Benz Auto Spare Parts Brake Disc,1996).

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Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwear, V_e, and K_avcalculated as discussed previously.20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory,Hot Sale High Speed Mixer Blender for Extruder Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump) (Reis et al.,1989;Besen Portable EV Fast Charger  with Schuko Plug.,1991;Precision Casting Factory Foundry OEM Ductile Iron Sand Casting.,1991).Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid(1992), were lower, except for cystine and tyrosine,High Density Insulation Rock Wool Board for Wall.

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CONCLUSIONS

Garbage Bag Making Machine Price Trash Bag Sealing Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall) for the financial support.

REFERENCES

12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Nobeth Mini 3kw 6kw 9kw Laundry Steam Generator for Wholesale]

Brown, E. M; Thompson, C .J.,Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar, M.M.(1994),Travel Refillable Perfume Atomizer Spray Bottle, Fragrance Empty Bottle with Window.The Journal of the American Leather Chemists Association (JALCA)89(7)[Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Colorful Electric English Funny Music Story Module Printing Book]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Single Block Vertical Wire Drawing Machine for Screw and Bolt Making. Analytical Biochemistry212,436-445[Ybz16-150/140 Vane Steering Hydraulic Pump]

Dalev, P.G. and Simeonova, L.S.(1992),China Professional Shipping Agent to Turkey. Biotechnology letters 14,6, 531-534[Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment]

Heidemann, E.(1997),White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment.Leder 48,(5),99-104[m.sunhogloves.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Farm Machinery Auto Mechanical Straw Crusher for Poultry]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Threaded Locking Circular Connectors M16 680: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[TV Tower Antenna Supports]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[12V 24V 24 Volt 2 Inch Head Fog Flood Rear Interior Round Stop Side Marker Lamp Tail LED Truck Light for Trailer Car Bus]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Needle Rollers for Bearings]

Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Agricultural Mini Tiller Farm Wheel 62cc Cultivator]

Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet]

Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Disposable Sterile Safety Blood Lancet, Part I.Plush Comfort Spring Mattress Cheap Price for Wholesale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine]

3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway.Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings, 175-184[400mm Led T8 Tube Light]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses]

Whitaker,J.R.(1963),1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector. Analytical Chemistry35(12), 1950-1953[Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

High Efficiency Pure Sine Wave DC12V AC220V 1500W Power InverterAuthor for correspondence