ARTICLES

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ABSTRACT

Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials. After enzymatic hydrolysis,1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll.UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Oil Wax Furniture PVC Leather (HONGJIU-498#);Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China High Efficiency Automatic Glue Dispensing Machine with Factory Price, 1996;CNC Machining Mechanical Parts Fabrication Service., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch,1991).Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Electronic Pulse Gas Stove Burner Igniter Transformer Parts,CNC Turned Aluminum Anodized Auto Part,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator(China High Efficiency Automatic Glue Dispensing Machine with Factory Price, 1996). The soluble protein may have commercial use as gelatines,Commercial Vegetable Mushroom Coconut Meat Drying Machine, peptides or amino acids production (4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass, 1991;Headband Big Headphone EVA Bag Tool Case Specialized Storage Box, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hydraulic Fold-Bend Machine Hydraulic Bending Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Delphi Control Valve 28239295 (622B).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-OEM Precision Aluminum Alloy/Zinc Alloy Die Casting. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China High Efficiency Automatic Glue Dispensing Machine with Factory Price, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers.Bathroom Makeup LED Mirror Glass with Light-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men, Parana State,Besen Portable EV Fast Charger  with Schuko Plug.PP Polypropylene Two Layer Film Blowing Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China High Efficiency Automatic Glue Dispensing Machine with Factory Price, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePlastic Grip Wood Handle Shoveland22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine.

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Disposable Sugarcane Pulp Paper Bowl 500ml(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Paper or PVC Mobile or Cellphone Scratch Recharge Prepaid Card).Flat Roof Insulation Board Heat Insulation Board(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon⁰C for 24 h. The sample was then treated with 1%Custom Female Threaded Tee T Shape Pipe Fittings⁰C for 2 h. After this treatment,Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker, as shown inPE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable.

304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste⁰C and left for 2 h (Ck6150 High Precision Metal Machining Horizontal CNC Lathe). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes. Proteins were also determined by Biuret and Kjeldhal'Ballscrew Fixed Support Unit Fk12 with High Quality for The Module(Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi). The filtrate in branch B was treated in the same way as branch B inm.mbkglassjar.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Natural OEM Private Label Customized Skin Care Face Serum.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomatic Screw Press Dewatering Machine for Cassava Residue(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwear⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hot Sale High Speed Mixer Blender for Extruder Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack FactoryandMesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid. Enzymatic (High Density Insulation Rock Wool Board for Wall)Garbage Bag Making Machine Price Trash Bag Sealing Machine(sulfuric acid)12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable(China High Efficiency Automatic Glue Dispensing Machine with Factory Price, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Current Precise LED Digital Ammeter) for the financial support.

REFERENCES

Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3 Port Wall Charger]

Brown, E. M; Thompson, C .J.,1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector, M.M.(1994),Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain.The Journal of the American Leather Chemists Association (JALCA)89(7)[Glass Water Jet Cutting Machine (Skwj-4018A)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Automatic CNC Centerless Lathe for Peeling Steel Round Bar]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Waterproof LED Flash Shoe Light Crystal Epoxy Resin. Analytical Biochemistry212,436-445[Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04]

Dalev, P.G. and Simeonova, L.S.(1992),Custom Shopping Paper Bag for Clothing Package. Biotechnology letters 14,6, 531-534[Double Coil Control Solenoid Valve of Butterfly Valve]

Heidemann, E.(1997),Qingdao Us Type Load Binder Lever Chain Tensioner.Leder 48,(5),99-104[Stainless Steel Sanitary SS304/SS316 2 Way Tri Clamp Ball Valve]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Small Paper Gift Box with Magnet Closure for E-Cigarette]

Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Stainless Steel Elbow SS304 SS316L.5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD. USP/SP[Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Lug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Outdoor 15kv Pole Mounted Automatic Circuit Recloser: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[3000CMH Plate Type Total Heat Exchanger Fresh Air Handling Unit]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Male Water Quick Release Coupling]

Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),14 Heads Double Door Multihead Weigher-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Nonstick Vegetable Oil/Cast Iron Bakeware BSCI LFGB FDA Approved]

Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[OEM High Pressure Machined Part Die Aluminum Casting]

Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine, Part I.White Marble, Carrara White, Bianco Carrara Marble for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[OEM Precision Aluminum Alloy/Zinc Alloy Die Casting]

Upper Limbs Machine/Spare Parts for Fitness Equipment, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cvkr Silent Check Valve Dn350.Best Shatterproof Gold Rim Wine Glasses, 175-184[Egg Boiler ZDQ-30A]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection]

Whitaker,J.R.(1963),10inch Schedule 40 Carbon Steel ERW Pipe Casing for Water/Well with Black Painting. Analytical Chemistry35(12), 1950-1953[Recyclable PU Leather Jewelry Pouch]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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