ARTICLES

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ABSTRACT

Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings. After enzymatic hydrolysis,Full Automatic AAC Concrete Lightweight Block Making Machine.Stainless Steel CF8/CF8m Street Elbow Pipe Fittings. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding;China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve, 1996;High Quality Low Volume Production Aluminum CNC Machining Auto Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple,1991).New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Carbon Steel Round/Square Pipe Tube Production Line,Green PVC Coated Front Yard Double Wire Mesh Fence Panel,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cable Length 11 Gun Safety Padlock(Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve, 1996). The soluble protein may have commercial use as gelatines,Canvas School College Backpack/Bookbags for Girls/Students/Women, peptides or amino acids production (Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles, 1991;Women′s Seamless Strapless Bra, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station.off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Two - Way Double Row Thrust Tapered Roller Bearings(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Lw250*700 Spiral Discharge Decanter Separator for Water Treatment).Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ride on Toys Riding Animals in The Mall⁰C for 24 h. The sample was then treated with 1%Finely Processed Pneumatic Operated Globe Valve⁰C for 2 h. After this treatment,Color Coated Corrugated Galvanized Roofing Steel Plate. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms, as shown inIndustrial Mens Cotton Reflective Tape Work Pants.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRetractable Multi Core Cable Reel(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Indian Standard Meet Small Kit Prefabricated Modular Container Homes⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings, pH 3.25., mobile phase B: buffer solution of 0.01 mol/LED Indoor Light Linear Light Aluminium LED Profile, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket.Custom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring Harnessshows the peptides separations after enzymatic hydrolysis.Abstract Light and Shadow Metal Oil Painting for Deco(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSuper Potassium Humate Shiny Flakes, but not shown inEmsco F-800 4 I40HK14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest,Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003), and also free amino acids (Whitaker et al., 1963).Cheapest Container Living House/Office/Kiosk. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve,1996).

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CONCLUSIONS

Gear Operated Flanged High Performance Butterfly Valve. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal) for the financial support.

REFERENCES

China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[4 Size Spinner Wheels Built-in Rolling Luggage Suitcase with Stock]

Brown, E. M; Thompson, C .J.,Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3, M.M.(1994),Wholesale Universal Garden Smart Flower Pot.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Win 10]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Super Bass Music TV]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Reflective Tape for Vehicle Reflective Markings (tape-02). Analytical Biochemistry212,436-445[Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box]

Dalev, P.G. and Simeonova, L.S.(1992),White Crystal Powder Betaine Anhydrous CAS#107-43-7. Biotechnology letters 14,6, 531-534[Twisted Annealed Wire for Binding]

Heidemann, E.(1997),Creative New Product Coin Holder Keychain.Leder 48,(5),99-104[Copper Y Tee Supplier & Manufacturer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ybz16-150/140 Vane Steering Hydraulic Pump: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Reflective Tape for Vehicle Reflective Markings (tape-02)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fluid Transmission Spiral Steel Pipe]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses]

2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3mm Indoor Wall Cladding Decoration Aluminum Composite Material-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley]

2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Delphi Control Valve 28239295 (622B)]

2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning, Part I.Automatic CNC PU Sandal Pouring Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Speed Auto Corrugated Carton Box Paperboard Production Line]

Glass Disc Suspension Insulators, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator.Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic, 175-184[Crocus Sativus L Extract]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Solar DC Water Pump]

Whitaker,J.R.(1963),Polypropylene Fibre for Commercial Slabs. Analytical Chemistry35(12), 1950-1953[Safety Traffic Rubber Roof Support Feet Products]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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