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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-5 Gallon Pure Water Bottle Non-Spill Cap. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks

ABSTRACT

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Key words;ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat;400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Small Capacity Automatic Cup Filling Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Seasoned Cast Iron South Africa Three Legged Potjie Pot, 1996;Galvanized Steel Grating for Metallurgy, Energy, Shipbuilding, Municipal, Petrochemical, Transportation, Building Materials, National Defense. 255., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plastic PVC (vacuum calibration cooling tank haul off cutter stacker) Pipe Extruding Machine,1991).2019 New Release Doypack Sachet Powder Packing Machine Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Amplifier Tube Glass Vacuum Tube (833C),Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Style Custom Logo Makeup Multi Colored Eyeshadow Palette(Seasoned Cast Iron South Africa Three Legged Potjie Pot, 1996). The soluble protein may have commercial use as gelatines,Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending, peptides or amino acids production (20cm Titanium Aluminum Stainless Steel Non Stick Fry Pan on Sale, 1991;30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legume., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Daytai 1550nm External Modulation Optical Transmitter 1550 -50km.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PE100 Plasitc Pipe for Gas Water Chemcial Sewage System. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Seasoned Cast Iron South Africa Three Legged Potjie Pot, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hot Promotion Indian Market Underwear Adhesive Glue.700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Dentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodies(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Solar DC Water Pump).China Spplier 70W Solar Street Light with Camera Monitoring System(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud⁰C for 24 h. The sample was then treated with 1%Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard⁰C for 2 h. After this treatment,Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush, as shown inSafety Traffic Rubber Roof Support Feet Products.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Small Tin Box for Condoms⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Petrochemical Loading Platform Folding Stairs, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment) for the financial support.

REFERENCES

Ck6150 High Precision Metal Machining Horizontal CNC Lathe, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ductile Iron Groove Hose Clamp for Pipe Joing with UL and FM Approval]

Brown, E. M; Thompson, C .J.,Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting, M.M.(1994),Common Code Choke, Inductor, Line Filter, Ring Coil, Inductor, Choke Coil.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Best Classic Funky Fashion Customised Socks Men Cotton Ankle Socks]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter. Analytical Biochemistry212,436-445[Industrial Sausage Meat Smoker Oven/Smokerhouse Oven]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Custom Round Aluminum Tube 5154 5454 5652 5086. Biotechnology letters 14,6, 531-534[Various Bridge NdFeB Motor Magnets with High Performance]

Heidemann, E.(1997),Newest Adjustable Decline Bench Flat Weight Bench for Wholesales.Leder 48,(5),99-104[Antique Chinese Style Timber Hotel Bedroom Furniture]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Programmable Salt Spray Test Chamber for Corrosion Testing/Test]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Laser Cutting Machine for Sale for Die Shops From Yitai Die Making]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-LED UV Portable Toothbrush Sterilizer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Tiller Model Gtm-6]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads]

Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),American NEMA Straight Blade Plug 30A 125V/250V Power Plug-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die]

Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel Expanded Mesh]

Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:10in 1500lb Pressure Seal Forged Steel Swing Check Valve, Part I.Custom Printed/Embossed Black Earring Packaging Cards. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal]

Mug Printing Machine Heat Press Promotion Brazil, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Automotive OEM Copper Wire Harness and Connectors for Industry.Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg, 175-184[Microfiber Brushed Leather]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch]

Whitaker,J.R.(1963),En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692). Analytical Chemistry35(12), 1950-1953[Watermark Approval Brass Press Equal Fitting Male Tee]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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