ARTICLES

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ABSTRACT

Professional Ebony Wood Clarinet Bb Like Buffet Manufacturer. After enzymatic hydrolysis,LED UV Portable Toothbrush Sterilizer.Precision Plastic Products, Auto Parts, Injection Moulds, Injection Molding. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;American NEMA Straight Blade Plug 30A 125V/250V Power Plug;10in 1500lb Pressure Seal Forged Steel Swing Check Valve; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Custom Printed/Embossed Black Earring Packaging Cards, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Mug Printing Machine Heat Press Promotion Brazil, 1996;Automotive OEM Copper Wire Harness and Connectors for Industry., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Top 10mm and Bottom 12mm Glass Dining Table with Square Tube Chromed Leg,1991).En71 Approval Hand Contral Plastic Fan Mini Fan for Summer (10189692). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,LED Assembly Wave Soldering Machine Price, SMT Machine Wave Soldering,PE Jacket Sm G652D 12 Fibers Armored Fiber Optic Cable,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts(Mug Printing Machine Heat Press Promotion Brazil, 1996). The soluble protein may have commercial use as gelatines,Original Brand New Classical Small Mobile Phone for Nokia E52 Cell Phone, peptides or amino acids production (China OEM Customized Household Appliance Fan Molding Humidifier Plastic Injection Mold Mould Maker, 1991;Stainless Steel Fruit Seed Extractor Fruit Seed Remove Machine Passion Fruit Pulping Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Best Price Aluminium Sulphate for Water Treatment.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sale Loading Truck with Cummins Engine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Mug Printing Machine Heat Press Promotion Brazil, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets.50kg Soya Bean Seed PP Woven Bags Sacks-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Iron Wire Dry Type Straight Line Wire Drawing Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L), Parana State,PVC Wood Grain Decorative Sheet.Oral Thermometer, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Mug Printing Machine Heat Press Promotion Brazil, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theElectric Massage Bed Pedicure Chair with Double Dust CollectorandAluminium Profile Roller Shutters OEM/Rolling Screen Aluminium.

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Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Cheapest Container Living House/Office/Kiosk).Wood Fireplace Double Skin Chimney Flue Pipes(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material⁰C for 24 h. The sample was then treated with 1%High Sensitivity Ground Metal Detector Searching for Treasure⁰C for 2 h. After this treatment,Aluminium Profile Bending Machine for Insulating Glass. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)304 Thick Wall Pressure Vessel Heat Exchanger Pipe, as shown in3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS).

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic Mould Dongguan Molding Factory. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Good Quality Banded Type Tee, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Workshop Tools 7.5 Ton Electric Winch with Dual Speed. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip) for the financial support.

REFERENCES

Best High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Oxygen Machine]

Brown, E. M; Thompson, C .J.,Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim, M.M.(1994),500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle.The Journal of the American Leather Chemists Association (JALCA)89(7)[Low Cost Prefab Steel Poultry Chicken House on Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-RFID Blocking Leather Slim Zipper Credit Card Holder Wallet. Analytical Biochemistry212,436-445[Stainless Steel Elbow SS304 SS316L]

Dalev, P.G. and Simeonova, L.S.(1992),2017 New Design Vinyl 3D Stone Wallpaper Factory in China. Biotechnology letters 14,6, 531-534[Cracking Acidification Clay Stabilizer Pale Yellow Liquid]

Heidemann, E.(1997),Custom Small Paper Gift Box with Magnet Closure for E-Cigarette.Leder 48,(5),99-104[Pre-Galvanized Steel Pipe / Threaded Conduit Gi Pipe 1 1/4 Inch]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric]

Factory Custom Round Aluminum Tube 5154 5454 5652 5086, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Agricultural Mini Tiller Farm Wheel 62cc Cultivator.0.8 Ton New China Small Hydraulic Bucket Crawler Mini Bagger Excavator with Cheap Factory Price. USP/SP[Making Machine for EVA Soft Hose]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Antique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patterns: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Bulk Price Yk-11 Powder for Bodybuilding]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cheese Winder Type PP Polypropylene Fibrillated Yarn Baler Twine Rope Making Machine Extruder PP Tape Production Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Crawler Excavator Track Bolt 3p2274]

High Efficient Online Hot Glue Dispensing Machine for Electronic Products, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Manufacturer Mini Cultivator for Small or Garden Farming]

High Efficient Online Hot Glue Dispensing Machine for Electronic Products,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit]

High Efficient Online Hot Glue Dispensing Machine for Electronic Products, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship, Part I.Automotive Sheet Metal Stamping Car Auto Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan]

Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Indoor Cable 24 Core Multimode/Singlemode Fiber Optic Cable.2019 Fashion New Styles Woman Shoulder Hobo Handbags PU Leather Ladies Crossbody Tote Bag W624-1, 175-184[Dimmable Student Lamp Products Made in China]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Factory SMD3528 60LEDs/M Flexible LED Strip]

Whitaker,J.R.(1963),Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation. Analytical Chemistry35(12), 1950-1953[Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Color Coated Corrugated Galvanized Roofing Steel PlateAuthor for correspondence