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ABSTRACT

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Key words;High Quanlity Colorful Aluminum Spacer/Standoff;5g OPP Silica Gel Desiccant; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Electric Winch With Groove Drum, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump, 1996;Truck Air Dryer cartridge 14bar., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors,1991).3mm White Waterproof PVC Foam Board. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Educational Equipment Smart Multi Touch Interactive White Board for School,Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Women Party Jewelry 45cm Sequins Choker(Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump, 1996). The soluble protein may have commercial use as gelatines,New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity, peptides or amino acids production (High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe, 1991;High Freqency PCB with Blind Via, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Strong Aluminum Armrest Reclining Hydraulic Barber Chair., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Factory SMD3528 60LEDs/M Flexible LED Strip.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot-Press Small Continuous Diamond Saw Blade Cutting Disc. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage.Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Comfort Furniture Natural Spring Memory Foam Mattress(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947⁰C and left for 2 h (Oil Wax Furniture PVC Leather (HONGJIU-498#)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles. Proteins were also determined by Biuret and Kjeldhal'Cog Mono Round Display LCD Module(Electric Winch With Groove Drum). The filtrate in branch B was treated in the same way as branch B in150w Street Light. The final soluble protein shown were used for peptides separations using gel permeation chromatography,4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avChina Products Suppliers Camlock Coupling Quick Couplings Female(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.3.2m Vertical Wheelchair Elevator Platform for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/LC Multistage Pump Mechanical Seal (KLLC), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wedding Tent Cooling System Outdoor Event Air-Con Units, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

220V/50Hz Stainless Steel Ozone Generator with 5g/H. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ferrous Sulphate Monohydrate 30%Min Factory Wholesale) for the financial support.

REFERENCES

Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[New Scba Breathing Apparatus Equipment with Ce Certification]

Brown, E. M; Thompson, C .J.,Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic, M.M.(1994),Luxury Jewelry White Gold Plated Engagement Diamond Ring.The Journal of the American Leather Chemists Association (JALCA)89(7)[Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[10mm AISI304 Carbon Steel Balls Motor Bearing for Slide]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Free Sample Cheap Price Plastic Black Underwear Hanger for Sale. Analytical Biochemistry212,436-445[Factory Custom 32 to 98 Infrared Transparent Video Wall Multi Touch Screen for Kiosk]

Dalev, P.G. and Simeonova, L.S.(1992),13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay. Biotechnology letters 14,6, 531-534[Threaded Locking Circular Connectors M16 680]

Heidemann, E.(1997),100% Cotton Face Mask in Guangzhou.Leder 48,(5),99-104[Roof Making Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[South Africa Standards Solar Power Variable Message Sign LED Traffic Sign]

Plug-in Assembly Type Straight Through Joint and Insulation Joint, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),OEM High Pressure Machined Part Die Aluminum Casting.AISI 430 Ba/2b Embossed Stainless Steel Sheet. USP/SP[Wedding Tent Cooling System Outdoor Event Air-Con Units]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[LC Multistage Pump Mechanical Seal (KLLC)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Food Delivery Thermal Bags]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Full Viton Coated Half Shaft Wafer Butterfly Valve]

Drug Delivery ABS Hospital Medical Treatment Medicine Cart, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR)]

Drug Delivery ABS Hospital Medical Treatment Medicine Cart,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Nail Stamping Plates Custom Metal Part Sheet Metal Stamping]

Drug Delivery ABS Hospital Medical Treatment Medicine Cart, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress, Part I.Colorful Electric English Funny Music Story Module Printing Book. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat]

2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650).Tilt-and-Turn-with-Hidden-Hinge, 175-184[VIA]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Seasoned Cast Iron South Africa Three Legged Potjie Pot]

Whitaker,J.R.(1963),Automatic Soft PVC Granule Used for Refrigerator Door Gasket. Analytical Chemistry35(12), 1950-1953[Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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