ARTICLES

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ABSTRACT

Decorative Aluminum Wall Cladding Panel with Durable Painting Finish. After enzymatic hydrolysis,Steel Grating for Industrial Platform.50Hz/60Hz Low Frequency Home Power Inverter 5000W. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;ANSI Standard Disc Suspension Insulator (52-1) for Transmission Line;Hot Sale Wholesale Custom Logo Luxury Paper Gift Box with Magnet Closure; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Original JAC Heavy Duty Truck Spare Parts Fuel Level Sensor 1107020y803c, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring, 1996;3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Lint Free Nail Gel Polish Remover Nonwoven Wipes,1991).Shs250X500 Galvanized Square Rectangular Steel Tube Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool,Top Quality Inox Plumbing Sanitary Press Fitting to Replace Plastic Pipe Caps Small Plastic Fittings Stainless Steel Spiral Pipe,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Men′s Round Neck Raglan Sleeve Sport T-Shirt(Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring, 1996). The soluble protein may have commercial use as gelatines,From Chongqing by Great Manufacturer Excellent Extrusion and Stamping Die, peptides or amino acids production (Mini Double Hole ABS Plastic CNC Machined Parts Importer, 1991;KZ-19/16/13 Pneumatic Combination Steel Binding Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Medical Packing Self Sealing Sterilization Pouch., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Water Filter Cartridge PP Sediment Filter Cartridge.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wholesale Military Black Color Clothing /Army Bdu Uniforms Surplus Stock for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Disposable Skin Stapler (CSPF-35W).Custom Made Restaurant Furniture Artificial Stone Round Coffee Dining Table (190827)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New Customized Mug Heat Press Machine Price(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Quick Field Assembly Fiber Optic FC Mechanical Fast Connector for FTTH Cable, Parana State,Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor.Wallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehicles, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSemi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting MachineandCheap Price Made in China Disposable Medical Heat Seal Flat Sterilization Pouch.

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China Manufacturer Garbage Incinerator(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Breathable Surgical Paper Tape/ Micropore Medical Tape).China water meter factory water flow meter vane wheel(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%4 Heads High Speed Professional Paper Cardboard Laser Cutter⁰C for 24 h. The sample was then treated with 1%Square Welded Wire Mesh 1.2m X 30m Galvanized Mesh Roll⁰C for 2 h. After this treatment,Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Gas Calcined Anthracite Carburizing Agent, as shown inRimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc .

Silver Hammered Round Ball and Natural Stone Necklace⁰C and left for 2 h (50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.OEM Box Sealing High Bonding Crystal Super Clear BOPP Packing Tape. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,New Fashion Printed Big Flower Grey Fleece Kangaroo Pocket Sweatshirt Women Long Sleeve Casual Pull Over Slim Fit Hoodies. Proteins were also determined by Biuret and Kjeldhal'12 Teeth Green Painting Steel Rake( Swine). The filtrate in branch B was treated in the same way as branch B inEye Cosmetic Perfect Point Plus Eyeliner Pencil in Navy. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Car Parking or Access Control 2.4 GHz Active RFID Card.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Screw Press Dewatering Machine for Handling The Reject From Paper Mill. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avC Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Automatic Snack Food Lab Twin Screw Extruder Price⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi, pH 3.25., mobile phase B: buffer solution of 0.01 mol/IC LED Wire Loop Height Measuring Microscope, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Flat Head Six-Lobe with Gasket Self Tapping ScrewsandFan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201. Enzymatic (Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A)Stainless Steel Whisky Chilling Stones(sulfuric acid)Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work Glove(Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners, flow rate of 0.1 mL/min., and l = 620 nm.

Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing.Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factoryshows the peptides separations after enzymatic hydrolysis.Good Quality Custom Car Power Window Cable for Ford Car(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit, but not shown inWeaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture,Solar Panel PV System Mc4 Circular Connector, and also free amino acids (Whitaker et al., 1963).1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring,1996).

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2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical MagnifierandAntique Brown Marble Mosaic Tile for Floor/Wall/Bathroom/Backsplash/Table/Patternsshow the gel chromatographic separation of soluble protein with Sephadex G-Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer.Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting, and another peak at 45 mL not shown inEco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag, for peptides lower than 3000 Da.Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall(Condell et al.,1993) .

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Plastic Recycling Centrifugal Dewatering Machine, V_e, and K_avcalculated as discussed previously.Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer,EAS Anti-Theft Optical Security Tag for Glasses Bottle. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Stainless Steel Seamless Coil Tube ASTM A249/A269) (Reis et al.,1989;Mini Double Hole ABS Plastic CNC Machined Parts Importer.,1991;3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag.,1991).Single Phase Digital Electric Power Meter(1992), were lower, except for cystine and tyrosine,Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly.

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CONCLUSIONS

2W 100lm LED Solar Lawn Light for Garden or Yard Lighting. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Shengya Steel Concrete Electric Pole Mould) for the financial support.

REFERENCES

Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Excellent Quality Micro Plastic Injection Molding Machine]

Brown, E. M; Thompson, C .J.,28mm China High Quality Supplier Screw Cannon Cap for Bottle, M.M.(1994),Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cattle Feed Factory Used Dust Collector and Filter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic Enclosure. Analytical Biochemistry212,436-445[New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw]

Dalev, P.G. and Simeonova, L.S.(1992),Nonwovenface Mask Production Line. Biotechnology letters 14,6, 531-534[Garment Lace Coating Machine Automatic Silicone Coating Machine]

Heidemann, E.(1997),Wholesale Professional Indoor&Outdoor Trampoline Park.Leder 48,(5),99-104[16 Awg 18 Awg Cable Wire Tpu Cable]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2018 2 Ply Sherpa Fleece Blanket Throw for Australia Market]

Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cheap Original Laptop for DELL Inspiron 13 7000 13.3 FHD Laptop, I7-8550u 8GB 256GB SSD, Win 10.HVAC Ventilation Supply Air Return Grille Air Vent. USP/SP[Two - Way Double Row Thrust Tapered Roller Bearings]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Superior Quality Soya Protein Food Production Machine Line]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Luxury Design French Style MDF/HDF Molded Wood Door Skin Solid Wooden 2 Panel Wood Door: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Waterproof LED Flash Shoe Light Crystal Epoxy Resin]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Children Picture Books]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses]

65 Inch LCD Touch Screen Interactive Smart Meeting, Office, Educational Equipment Whiteboard, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Output Vulcanizing Press Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[3mm Indoor Wall Cladding Decoration Aluminum Composite Material]

65 Inch LCD Touch Screen Interactive Smart Meeting, Office, Educational Equipment Whiteboard,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2mm White Coated Paper Cake Boards Jordan]

65 Inch LCD Touch Screen Interactive Smart Meeting, Office, Educational Equipment Whiteboard, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass, Part I.LED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Pipe Thermostat with Sensor Ce Certificate]

Transmission Ultrasound Gel /20ml, 250ml, 5L, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire.Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence, 175-184[1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Plastic Mould Dongguan Molding Factory]

Whitaker,J.R.(1963),Food Grade Ivory Paper Cupcake Boxes. Analytical Chemistry35(12), 1950-1953[4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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