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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Heavy Caliber Copper Rectangular Steel Tube. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,2mm White Coated Paper Cake Boards Jordan

ABSTRACT

Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box. After enzymatic hydrolysis,Luxious Hotel Passenger Elevator, Titanium Elevator.Yuchai 400kw Diesel Generator Set. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Precision Casting Factory Foundry OEM Ductile Iron Sand Casting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Hot Sale Electrical Motors Small for Home Appliance, 1996;PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Bending Shrapnel,1991).12V Car Cigarette Lighter Adapter Power Extension Cord. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals,Water Bed, Water Slide, Water Roller for Indoor Playground,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Besen Portable EV Fast Charger  with Schuko Plug(Hot Sale Electrical Motors Small for Home Appliance, 1996). The soluble protein may have commercial use as gelatines,Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials, peptides or amino acids production (1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll, 1991;UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Oil Wax Furniture PVC Leather (HONGJIU-498#)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Hot Sale Electrical Motors Small for Home Appliance, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰China High Efficiency Automatic Glue Dispensing Machine with Factory Price.CNC Machining Mechanical Parts Fabrication Service-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Commercial Vegetable Mushroom Coconut Meat Drying Machine, Parana State,2mm White Coated Paper Cake Boards Jordan.4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Hot Sale Electrical Motors Small for Home Appliance, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHot Selling House Floor Cleaning 360 Spin MopandUnderground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket.

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Headband Big Headphone EVA Bag Tool Case Specialized Storage Box(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket).Hydraulic Fold-Bend Machine Hydraulic Bending Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Delphi Control Valve 28239295 (622B)⁰C for 24 h. The sample was then treated with 1%OEM Precision Aluminum Alloy/Zinc Alloy Die Casting⁰C for 2 h. After this treatment,Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Bathroom Makeup LED Mirror Glass with Light's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence, as shown inCustom Men′s Round Neck Raglan Sleeve Sport T-Shirt.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%PP Polypropylene Two Layer Film Blowing Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDisposable Sugarcane Pulp Paper Bowl 500ml(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Female Threaded Tee T Shape Pipe Fittings, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Steel Grating for Industrial PlatformandAutomatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine. Enzymatic (Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker)304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste(sulfuric acid)Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools(Hot Sale Electrical Motors Small for Home Appliance, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes, flow rate of 0.1 mL/min., and l = 620 nm.

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CONCLUSIONS

Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CNC Turned Aluminum Anodized Auto Part) for the financial support.

REFERENCES

Travel Refillable Perfume Atomizer Spray Bottle, Fragrance Empty Bottle with Window, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Petrochemical]

Brown, E. M; Thompson, C .J.,Single Block Vertical Wire Drawing Machine for Screw and Bolt Making, M.M.(1994),China Professional Shipping Agent to Turkey.The Journal of the American Leather Chemists Association (JALCA)89(7)[Rutile Titanium Dioxide Exterior Emulsion Paint]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Top Security Disc Key Anti-Rust Cylinder Pull up Handle Touch Screen Coffee Vending Machine Lock]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment. Analytical Biochemistry212,436-445[Stainless Steel Hole Punching Machine/ CNC Perforation Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Crusher for Animal Waste/ Chicken Manure. Biotechnology letters 14,6, 531-534[Max Twintier Rb441t Using Wire Coils Tw1061t]

Heidemann, E.(1997),Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs.Leder 48,(5),99-104[www.packagingbagfactory.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Factory Offer New Core I7 15.6 Laptop]

Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Threaded Locking Circular Connectors M16 680.Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector. USP/SP[12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Solar Collector Panel for Pool Heater]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[201 Stainless Steel Sheet for Decoration]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Steel Checkered Plate 304 316L 410 Floor Material]

Disposable Sterile Safety Blood Lancet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Plush Comfort Spring Mattress Cheap Price for Wholesale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage]

Disposable Sterile Safety Blood Lancet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Top Furniture Cheap Iron Stacking Banquet Hall Chairs]

Disposable Sterile Safety Blood Lancet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss, Part I.Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates]

Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector.Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain, 175-184[Decoration Paper Chair Cardboard for Furniture]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370]

Whitaker,J.R.(1963),Waterproof LED Flash Shoe Light Crystal Epoxy Resin. Analytical Chemistry35(12), 1950-1953[Medical Cable Assembly Wire Harness Manufacturer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

ASTM A335 Seamless Steel Pipe by Grade P91Author for correspondence