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ABSTRACT

Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat. After enzymatic hydrolysis,Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts.Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays;Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Samples Free! ! ! Primary/Remelted/Refined Lead Ingots, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo, 1996;120PCS/M Flexible LED Strip Tira De LED Light Strip., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring,1991).Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage,Factory Cheap Price Wholesale Black Nickel Bag O Ring Metal, Professional Custom 43mm Metal O-Ring for Bag,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11(Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo, 1996). The soluble protein may have commercial use as gelatines,High Quality Sesame Bean Seed Cleaner and Grader, peptides or amino acids production (Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag, 1991;Pet Strapping Band Production Line, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag, RFID Paper Label, Smart Label., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PA Flexible Plastic Corrugated Loom Tubing. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl.Belt Conveyor in Heavy Duty Industry for Mining, Coal, Power-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Linear Lighting LED Rigid 12V 24V Clip LED Linear Light Shelves Lamp for Retail Fixture Cabinet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCarbon Steel Round/Square Pipe Tube Production Line(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cable Length 11 Gun Safety Padlock⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Canvas School College Backpack/Bookbags for Girls/Students/Women, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Color Coated Corrugated Galvanized Roofing Steel Plate. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms, M.M.(1994),Precision Diamond Wire Saw Rope for Cutting Concrete Stone.The Journal of the American Leather Chemists Association (JALCA)89(7)[Rutile Anatase Titanium Dioxide for Coating and Paints]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pneumatic Shuttle Valve Flange Ends]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty. Analytical Biochemistry212,436-445[Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag]

Dalev, P.G. and Simeonova, L.S.(1992),Blince High Torque OMR Hydro Motor for Injection Moulding Machine. Biotechnology letters 14,6, 531-534[Quick Field Assembly Fiber Optic FC Mechanical Fast Connector for FTTH Cable]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Full-Automatic Folding Napkin Paper Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Retractable Multi Core Cable Reel: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China OEM ABS Clear Plastic Injection Molding]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pringing Industry Wastewater Treatment Plant]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Soda Ash Dense]

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New Xcm 120 Asphalt Mixing Plant for Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box]

New Xcm 120 Asphalt Mixing Plant for Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings, Part I.LED Indoor Light Linear Light Aluminium LED Profile. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Carbide Mould Parts Moulding Parts]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cog Mono Round Display LCD Module]

Whitaker,J.R.(1963),Workbench Anti-Static Table ESD Natural Rubber Table Mat. Analytical Chemistry35(12), 1950-1953[Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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