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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Hot Selling House Floor Cleaning 360 Spin Mop. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles

ABSTRACT

Advanced Unlocking Tool for Locksmith. After enzymatic hydrolysis,High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts.Fashion Design Sleeveless Fitness Wear. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;DC Colorful Battery Operated Small Hand Fan for Outdoor Use;Hsc Heavy Duty 5 Ton Chain Pulley Block; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Outdoor Christmas Decoration Highway Street Light, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length, 1996;China Cheap Newest 70*77cm XPE Foam 3D Wallpaper., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (48V 200ah UPS Battery Backup for Telecom,1991).450ml Can Beer Tin Take Away 2 Pack Shipped on Beer Tray. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks,5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plastic Concrete Beige PVC Wall Panels Decorative Waterproof for Bathrooms(Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length, 1996). The soluble protein may have commercial use as gelatines,8L Mini Fridge Freezer for Home and Car Fridge, peptides or amino acids production (Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r, 1991;23m 4 R Arms 2017 New Design Concrete Placing Boom, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Factory Offer New Core I7 15.6 Laptop.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ce Approved Top Quality Hot-DIP Galvanized Pig Sow Farrowing Crates. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Mining Machinery Parts Diamond Saw Blade Segments for Marble.Releasable/Resuable Stainless Steel Metalic Tie Strap for Binding-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Plastic Grid for Paver Grass for Gardening(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Forging Metal Building Parts, Parana State,Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles.Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMaking Machine for EVA Soft HoseandAuto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008.

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Safety Traffic Rubber Roof Support Feet Products(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Crawler Excavator Track Bolt 3p2274).Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology⁰C for 24 h. The sample was then treated with 1%Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter⁰C for 2 h. After this treatment,Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor, as shown inChina Manufacturer Mini Cultivator for Small or Garden Farming.

Rubber Tire Plastic Recycling Shredder with Factory Price⁰C and left for 2 h (700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile. Proteins were also determined by Biuret and Kjeldhal'Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data(Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan). The filtrate in branch B was treated in the same way as branch B inwww.yxmembrane.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Industrial Mens Cotton Reflective Tape Work Pants.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAluminum Coil with AA3003 AA3105 H14 H16 H18(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.DC Stand Fan Motor PCBA Card 24V⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Economic Brwon Kraft Slip Sheet Tray and Pallet Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel 304 Wire Rope Fork Terminal, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator.Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Moldshows the peptides separations after enzymatic hydrolysis.High Quality Good Design Custom Made Cheap Recycle Paper Bags(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inColor Coated Corrugated Galvanized Roofing Steel Plate, but not shown inDedi 43′′ 55′′ Outdoor LCD Display Screen with Camera,Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White, and also free amino acids (Whitaker et al., 1963).Non-Ionic Polyacrylamide Flocculant. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length,1996).

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CONCLUSIONS

Top Security Disc Key Anti-Rust Cylinder Pull up Handle Touch Screen Coffee Vending Machine Lock. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair) for the financial support.

REFERENCES

Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Construction Hoist Anti-Fall High Quality Products]

Brown, E. M; Thompson, C .J.,Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board, M.M.(1994),High Precision Green Sand Casting Ball Valve Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[Pneumatic Shuttle Valve Flange Ends]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Bypass Shower Door Screen with Stainless Steel 304 Frame Hardware. Analytical Biochemistry212,436-445[Quick Field Assembly Fiber Optic FC Mechanical Fast Connector for FTTH Cable]

Dalev, P.G. and Simeonova, L.S.(1992),Class 300 1/2 Stainless Steel Forging Type Check Valve. Biotechnology letters 14,6, 531-534[Full-Automatic Folding Napkin Paper Machine]

Heidemann, E.(1997),CAD Cam Dental Machine 3D Dental Model Printer.Leder 48,(5),99-104[China Digital Ph Controller]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Magnetic Latching Relay for Health Life]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China OEM ABS Clear Plastic Injection Molding]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Best Selling Wooden Grain Metal Aluminium Fence Panels for Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Soda Ash Dense]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Electro Galvanized Wire/Galvanized Iron Wire/Gi Binding Wire]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality 45W 50W Commercial Recessed COB LED Downlight]

5 Liter Packed Medical Ultrasound Gel with Ce and ISO, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box]

5 Liter Packed Medical Ultrasound Gel with Ce and ISO,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Carbide Mould Parts Moulding Parts]

5 Liter Packed Medical Ultrasound Gel with Ce and ISO, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Selling Porcelain White Coffee Mug with Electroplate Handle Custome Printed Character, Part I.Etched Stainless Steel Titanium Sheet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cog Mono Round Display LCD Module]

Heat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass Fabric, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed.Cold Rolled Galvanized Carbon Hot Welding Stainless Steel Pipe Round Seamless Stainless Steel Tube (403/408/409/410/416/420/430/431/440/440A/440B/440C/439/443), 175-184[Wastewater Sludge Treatment Rotary Drum Filter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%]

Whitaker,J.R.(1963),33kv Electrical Automatic Voltage Regulator. Analytical Chemistry35(12), 1950-1953[China Quality Heavy Duty Globe Control Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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