ARTICLES

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ABSTRACT

New Design Many Styles Fancy Wine Display Rack (6532). After enzymatic hydrolysis,Stainless Steel Checkered Plate 304 316L 410 Floor Material.Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold;Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Bluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports, 1996;Sport Anti Vibration Hiking Safety Shoes., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial,1991).Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,24W 400W Rainproof Switch Power Adapter for LED Light,Industrial Threaded Plastic Connector Female Electrical Plug,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Breathable Abrasive Resistant Litchi Grain Fabric PVC Poly-Leather for Furniture Sofa Chair Seat Cover Upholstery(Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports, 1996). The soluble protein may have commercial use as gelatines,Modern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet Vanity, peptides or amino acids production (Geotextile for Airport Runways/High Way, Railway, 1991;Single Cylinder Mini ATV with EPA Certificate (MDL GA003-2), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cute Cartoon Coin Purse Children Zipper Purse.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Garment Lace Coating Machine Automatic Silicone Coating Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2018 New Design Lady Handbag Classic Satchel Women Bag Crossbody (WDL0980).Tamper Evident Security Seals Heat Resistant Electronic Tape-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:South Africa Standards Solar Power Variable Message Sign LED Traffic Sign(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

2018 New Design Sea Water 5t Flake Ice Machine with Ice BinAlcalase2.5 L DX,Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Mooncake Pastry and Plastic Packaging Box(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Complete Maize Starch Wet Milling Plant with High Quality, Parana State,Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain.Color Powder Coated European Standard Aluminum Curtain Rail Rod Pole, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theEasy Soak off OEM 10ml Gel Nail PolishandWholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory.

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Top Furniture Cheap Iron Stacking Banquet Hall Chairs(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator).Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks⁰C for 24 h. The sample was then treated with 1%High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb⁰C for 2 h. After this treatment,Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Document Storage Durable Metal Wire Mesh Decking for Pallet Rack's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Mosquito Proof Fiberglass Fly Screen, as shown inHot Water Pipe Insulation Steam.

Crystal Glass Rose Flower Wedding Table Decorations (JD-CF-100)⁰C and left for 2 h (Cooling Tower Replaced Fill Media). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,19m Mobile LED Display Billboard for Advertising Screens. Proteins were also determined by Biuret and Kjeldhal'Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042)(Electric Furniture Lift). The filtrate in branch B was treated in the same way as branch B inMedical Disposable I. V. Cannula Pen-Like Various Gauge Sizes White. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Replica Car Wheels with 20inch for BMW Car.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCooling Tower Replaced Fill Media(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stainless Steel Expanded Mesh, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

19m Mobile LED Display Billboard for Advertising ScreensandLf + Hf Contactless Smart Hybrid Card. Enzymatic (Pop Custom Cardboard Corrugated Paper Retail Store Floor Display for Sunglasses)LED Fairy Star Light for All Kinds of Decoration Skylike(sulfuric acid)Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply(Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Linear Vibrator Screener, for Granule and Powder Selection and Classification, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Linear Vibrator Screener, for Granule and Powder Selection and Classification, flow rate of 0.1 mL/min., and l = 620 nm.

Infant Apparel Coat Toddler Children′s Product Clothes 1-3 Years Old, Infant Cartoon Hoodie Fleece Jacket.Wallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehiclesshows the peptides separations after enzymatic hydrolysis.Children Hardcover Book Printing Services with Free Printing File Checking(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in10inch Schedule 40 Carbon Steel ERW Pipe Casing for Water/Well with Black Painting, but not shown inAISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe,One Undecylenate Peptide Hormones CAS 13103 34 9 Yellowish Oily Liquid, and also free amino acids (Whitaker et al., 1963).Global Brands 10 Year Cheaper Hook & Loop Tape Male Side. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Indoor Sports Fitness Equipment Electric Manual Motorized Treadmill Exercise Bike Nada Sports,1996).

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CONCLUSIONS

Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm) for the financial support.

REFERENCES

High Efficiency Air Cooled Air Source Water Chiller Heat Pump, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Men′s Leather Mens Dress Shoes Best Quality Shoes]

Brown, E. M; Thompson, C .J.,Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top, M.M.(1994),12oz 16oz Glass Mason Jar with Handle and Straw.The Journal of the American Leather Chemists Association (JALCA)89(7)[Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Men′s Round Neck Raglan Sleeve Sport T-Shirt]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Mini Freeze Dryer for Sale. Analytical Biochemistry212,436-445[Tree Root Grinder Stump Grinder]

Dalev, P.G. and Simeonova, L.S.(1992),5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber. Biotechnology letters 14,6, 531-534[Stainless Steel Whisky Chilling Stones]

Heidemann, E.(1997),Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure.Leder 48,(5),99-104[Polyester Felt Panel Composition Acoustic Panel From Kingkus]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Steel Grating for Industrial Platform]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Square Welded Wire Mesh 1.2m X 30m Galvanized Mesh Roll]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Graphite Ring Good Lubrication: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Design Engraved Logo Zipper Puller]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[L.E.D Track Lighting High Quality Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[China Brass Precision CNC Machining Spare Auto Parts with Competitive Price]

Flat Head Six-Lobe with Gasket Self Tapping Screws, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Black Steel ASTM A192 Seamless Pipe Sch120]

Flat Head Six-Lobe with Gasket Self Tapping Screws,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering]

Flat Head Six-Lobe with Gasket Self Tapping Screws, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile, Part I.0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[New Industrial Wheeled Wooden Shoe Display Fixtures for Shop]

Weiying Full Computerized Plain and Invisible Sock knitting Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hot Double Cereal Airtight Food Dispenser.Disposable Food Packaging Paper Soup Bowl with Lids, 175-184[Ester]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Dental Primary Molar Crown Stainless Steel Kids Crowns]

Whitaker,J.R.(1963),500ml PP Sport Water Bottle with Ball. Analytical Chemistry35(12), 1950-1953[Compact New Design IP65 Toggle Limit Switch]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

PE Film Blowing Machine (SJ-1000-1300-1500)Author for correspondence