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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Foteck SSR 40da Solid State Power SSR Relay. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,2015 New Square Folding Ajustable Wall LED Bathroom Mirror

ABSTRACT

Equipment Manufacturers Mayflay Sales Semi-Auto Dry - Suction Commercial Sandblaster for Motorcycle Frame Surface Cleaning with Light Gun & Nozzle. After enzymatic hydrolysis,13.8cm Reed Diffuser Clear Glass Bottle for Aroma.Metal Cutting Machine CNC Plasma Cutter. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Medical Suction Connecting Tube with Yankauer Suction Handle;Surface Mounted Wall Light for Home Decoration Waterproof; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Yhzs60 Mobile Concrete Batching Plant, Portable Concrete Mixing Plant, Portable Ready Mix Concrete Batch Plant, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Self-Propelled Wheel Farm Using 3 Rows Corn Harvester, 1996;Right Angle Worm Gearbox for Food and Beverage and Packaging Machines., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Manufacturer High Strength Carbon Steel Heavy Hex Nut,1991).Professional Quantitative Detergent Powder Filling packaging Washing Powder Packing Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Seamless Coil Tube ASTM A249/A269,The Cheapest Hot Sale Agricultural/Commercial Plastic Greenhouse (JIT-406021PT),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Quality Control High Purity 99% Ceftiofur Sodium Powder CAS104010-37-9 in Stock(Self-Propelled Wheel Farm Using 3 Rows Corn Harvester, 1996). The soluble protein may have commercial use as gelatines,Hardware Rigging Forged Red Weldless Pear Shaped Link, peptides or amino acids production (Hot Water Pipe Insulation Steam, 1991;Economy Combo Heat Press for Starter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Long Distance Metal/Plastic Impulse Sprinkler Farm Irrigation Garden Water Sprinkler., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Food Grade Beverage Clear PVC Tubing Hose.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Imee Printing Eco Friendly Promotional Drawstrings Craft Kraft Paper Cardboard Clothing Foldable Shopping Gift Bag. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Self-Propelled Wheel Farm Using 3 Rows Corn Harvester, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Wet and Wavy Remy Mink Brazilian Hair Weave Bundles Deep Wave.Luxury Plastic Pump Cosmetic Packaging as Airless Bottle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Top Quality Cheap Fire Resistant PVC Vinyl Flooring for Sports(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Frequency Online UPS W/3W-20K, Parana State,2015 New Square Folding Ajustable Wall LED Bathroom Mirror.Solar Collector Panel for Pool Heater, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Self-Propelled Wheel Farm Using 3 Rows Corn Harvester, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the8L Mini Fridge Freezer for Home and Car FridgeandPet and PP Geobag with UV High Strength Thermal.

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ASTM A500 Gr. a/B/C Steel Square Tubing Square Tubing Sizes From China Pipe Manufacturers(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (48V 200ah UPS Battery Backup for Telecom).Aluminum Sheet Metal Case Parts for Industrial Computer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Black Plastic Handle Stainless Steel Kitchen Gadget, Kitchen Tools Set⁰C for 24 h. The sample was then treated with 1%High Quality Hydraulic Cylinder Tubes for Hydraulic Cylinder⁰C for 2 h. After this treatment,RCA Audio Connector Male Gold-Plated Cable Jack Plug Male. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)A19 6W 10 Red & 4 Blue LED Plant Light LED Grow Light Bulb, as shown inSilo Machine Equipment for Chicken Farm Feed Use (JF-A-L003).

Premium quality for Potassium Humate powder⁰C and left for 2 h (Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Home Design Solid Wood Double Adult Bed Bedroom Furniture. Proteins were also determined by Biuret and Kjeldhal'Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont(New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65). The filtrate in branch B was treated in the same way as branch B inRotary Precision Metal Tailings Disc-Type Ceramic Vacuum Filter. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Dental Primary Molar Crown Stainless Steel Kids Crowns.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%304 Precision Professional Dimpled Stainless Steel Plate. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBoundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Prefabricated Steel Framed Sheds to Metro, Railway Station, Underground Carport, Airport, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Special Shape Spring with Good Quality and Fast Delivery, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Hot Sell Electro Hydraulic Synchronous CNC Press Brake Kcn-30032andLeather Wallet Slim Front Pocket Wallet Billfold ID Window RFID Blockingshow the gel chromatographic separation of soluble protein with Sephadex G-V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine.Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine, and another peak at 45 mL not shown inChildren Hardcover Book Printing Services with Free Printing File Checking, for peptides lower than 3000 Da.6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment(Condell et al.,1993) .

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CONCLUSIONS

T Slot Aluminum Extrusion Ob4040b Aluminium Profile, Industrial Aluminum Profile. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Sheet Metal Parts Stamping Processing Cold Rolling Plate Cutting Stamping Bending Precision) for the financial support.

REFERENCES

Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Flame Retardant Cargo Pants]

Brown, E. M; Thompson, C .J.,Max Twintier Rb441t Using Wire Coils Tw1061t, M.M.(1994),Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legume]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Test-630 Automatic Protective Relay Tester]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Manufacturer Supply Durable Grey Color Non-Slip EVA Mat. Analytical Biochemistry212,436-445[Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide]

Dalev, P.G. and Simeonova, L.S.(1992),Feed Storage OEM Grain Storage Silo 1cbm to 200cbm. Biotechnology letters 14,6, 531-534[Activated Alumina Used for Catalyst Carrier in Chemical Industry]

Heidemann, E.(1997),Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box.Leder 48,(5),99-104[One Tap Water Dispenser]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Flexible Stand up USB Charging Stands Cable Phone Charger Holder for Apple iPhone Samsung Huawei Android Phone Holders P0.11]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Atg39 100W LED Flood Light Wall Light Wall Washer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[48V Lithium Ion Battery 100ah 200ah LiFePO4 Battery Solar EV System]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest]

China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Gold Inlaid 24K Gold / Flowers-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set]

China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheels]

China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap, Part I.Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films]

Yashi 65inch Infrared Touch Whiteboard Interactive Smart Board, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Hydraulic Cutter Bread Machine (ZMF-20Y).9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board, 175-184[Ac 220v Relay]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile]

Whitaker,J.R.(1963),Yet905 Wireless Keypad Doorbell Kits with Remote Control. Analytical Chemistry35(12), 1950-1953[Strong Aluminum Armrest Reclining Hydraulic Barber Chair]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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