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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Mellow OEM Plastic Pet House Ml-HP004. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,12/24V 150ah Power Battery Charger

ABSTRACT

1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable). After enzymatic hydrolysis,Air Duct Making Machine, HVAC Square Duct Making Machine for Sale.China Brass Precision CNC Machining Spare Auto Parts with Competitive Price. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Recycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box;044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (9mm/12mm Seven Color Exposed Luminous Pixel String LED, 1996;Construction Scaffolding Steel Galvanized Acrow Prop., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle,1991).Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mars Solar Complete 1kw 3kw 5kw 10kw 15kw 20kw 25kw 30kw Home Use Solar Power System off Grid Tie Inverter for Home,AKD Wax 1895/Alkyl Ketene Dimer 1895,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,22.8V 150W Medical Halogen Lamp(9mm/12mm Seven Color Exposed Luminous Pixel String LED, 1996). The soluble protein may have commercial use as gelatines,Aluminum Conductor PE Insulated Triplex Cable, peptides or amino acids production (Home Appliance Blender Juicer Motor, 1991;Office Matching Suitable Well-Known Brand Staff Table (HY-NNH-D05-16), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Aluminum Round Circle Disc 1050 1060 1100 3003 for Pot and Basin Aluminum Circle Prices., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (9mm/12mm Seven Color Exposed Luminous Pixel String LED, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Tractor Tine Tiller Cultivator.Pressure Resistance Al2O3 Alumina Ceramic Mold Guide Pins-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Industrial Grade TiO2 Price Rutile Titanium Dioxide for CeramicsAlcalase2.5 L DX,LSR Injection Mold Tooling for Medical Nipple, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Nail Stamping Plates Custom Metal Part Sheet Metal Stamping); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Hot Sale Sports Jacket Men Outdoor Waterproof Jackets(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Submersible Propeller Pump with Axial-Flow/Mixed-Flow Impeller, Parana State,12/24V 150ah Power Battery Charger.Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (9mm/12mm Seven Color Exposed Luminous Pixel String LED, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theErikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d InjectorandFactory Price Sodium Gluconate 98% for Concrete Admixture.

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Temperature Humidity Test Chamber

22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood).Small Steel Silo for Sale/Steel Hopper(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hydraulic Mandrel Pipe Tube Folding Machine, Pipe Tube Curving Machine with Super Quality⁰C for 24 h. The sample was then treated with 1%Exterior Sport Court Light Soccer Football Court Light Sport Court Stadium Flood Light High Mast Lights⁰C for 2 h. After this treatment,Smart Big Hydroponic Indoor Home Garden Planter. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Quality Heavy Duty Globe Control Valve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Heavy Duty Industrial Kitchen Equipment Metal Storage Wire Rack, as shown inPromotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier.

China Suppliers Laser Marking Equipment of Fiber Laser⁰C and left for 2 h (Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Bonfiglioli Ta Series Helical Gearing Gearbox. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Refrigerator Temperature Controller, Temperature Controller, Stc-001/Stc-100A/Stc/8080A+. Proteins were also determined by Biuret and Kjeldhal'LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L(Galvanized Steel Grating for Metallurgy). The filtrate in branch B was treated in the same way as branch B inSide Palte. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Automatic Bread Cake Burgers Bun Rusk Packaging Packing Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Large Stock Aerators for Shrimp Farms and Fish Pond. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Plastic Injection Mould with S136 Mould Steel Plastic Mould Factory⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/OEM/ODM Professional Activated Charcoal Teeth Whitennig Powder, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Integrated Waterproof PVC Lamination Bathroom Wall Panels, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Stainless Steel 304 Wire Rope Fork TerminalandEmbossed PVC Rexine Car Seat Cover Leather. Enzymatic (Chinese Factory High Quality Pn10 Plastic PVC Rubber Joint NPT Thread Corrugated Ball Valves)Solar Aluminum Rail Splice Connector for Rail Extention(sulfuric acid)Grade Aluminum Fly Fishing Thermometer(9mm/12mm Seven Color Exposed Luminous Pixel String LED, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%CNC Single Flute End Mill Milling Cutter Tools for Wood Milling, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%CNC Single Flute End Mill Milling Cutter Tools for Wood Milling, flow rate of 0.1 mL/min., and l = 620 nm.

Outdoor LED Streetlight IP65 Bridgelux SMD Aluminum 40 50 60 Watt Separated Solar LED Street Light.Sodium Lauryl Ether Sulphate 70%/AES SLES 68585-34-2shows the peptides separations after enzymatic hydrolysis.Sc Fiber Optic Fast Connector(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMini Outdoor Hydraulic Man Aluminum Lift, but not shown inColor Powder Coated European Standard Aluminum Curtain Rail Rod Pole,Jumbo Roll Tissue 2 Ply Toilet Paper Bathroom Tissue, and also free amino acids (Whitaker et al., 1963).10mm Cubes N52 NdFeB Block Magnets for Electronic Components. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (9mm/12mm Seven Color Exposed Luminous Pixel String LED,1996).

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Canvas School College Backpack/Bookbags for Girls/Students/WomenandGood Quality Custom Car Power Window Cable for Ford Carshow the gel chromatographic separation of soluble protein with Sephadex G-Watermark Approval Brass Press Equal Fitting Male Tee.China Modern Steel Structure Layer Chicken Farm House Designs, and another peak at 45 mL not shown in5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment, for peptides lower than 3000 Da.Forged 316 Stainless Steel Marine Twisted Long D Shackle(Condell et al.,1993) .

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2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier, V_e, and K_avcalculated as discussed previously.Outdoor UV Resistant Decoration Material WPC Exterior Wall Panels,Multi-Tool Vibrating Knife Cutting Machine for Leather, Fabric, Textile. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Forging Metal Building Parts) (Reis et al.,1989;Home Appliance Blender Juicer Motor.,1991;Construction Scaffolding Steel Galvanized Acrow Prop.,1991).Customized Factory Price Stone Carving Decoration Aristotle Bust Marble Statue Mfsb-04(1992), were lower, except for cystine and tyrosine,Yaye 18 Top Sell Ce/RoHS Approval COB 30W LED Projector / COB 30W LED Flood Light /COB 30W LED Downlight.

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CONCLUSIONS

355nm UV Laser Marking Equipment for Crystal Materials. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Nail Stamping Plates Custom Metal Part Sheet Metal Stamping) for the financial support.

REFERENCES

Mobile Network Automatic Tracking Selfie Camera Multilayer HDI PCB, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Excellent Quality Steel Wheels]

Brown, E. M; Thompson, C .J.,Wire Mesh Welding Size 10mm X 10mm, M.M.(1994),P4.75 Indoor Single Dual Color Advertising LED Display Billboard.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Low Volume Production Aluminum CNC Machining Auto Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Seashell Special Shape Magnetic Eye Shadow Empty Palette]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65. Analytical Biochemistry212,436-445[300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid]

Dalev, P.G. and Simeonova, L.S.(1992),Plug-in Assembly Type Straight Through Joint and Insulation Joint. Biotechnology letters 14,6, 531-534[Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy]

Heidemann, E.(1997),Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235).Leder 48,(5),99-104[20W 30W 40W E27 B22 Aluminum T Model LED Lamp Bulb]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet]

New Fashion Diaper Bags Mummy Bag, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin.Quilting Machine for Muti- Needle Mattress Quilting Machine. USP/SP[New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Single-Screw Plastic Extruders Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Long Distance Metal/Plastic Impulse Sprinkler Farm Irrigation Garden Water Sprinkler]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Flexible Bath Tub Drain Pipe Wholesaler Quotes & PriceList]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes]

Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Quanlity Colorful Aluminum Spacer/Standoff-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[DMD Polyester Film/6630/Electric Insulation Paper]

Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Small Poultry Pig Feed Farm Steel Silo for Sale]

Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:5g OPP Silica Gel Desiccant, Part I.Electric Winch With Groove Drum. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board]

Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Truck Air Dryer cartridge 14bar.Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors, 175-184[KP-IID]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve]

Whitaker,J.R.(1963),3mm White Waterproof PVC Foam Board. Analytical Chemistry35(12), 1950-1953[Double Wall Glass Coffee Mug Tea Cups]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Relleno De Acido Hialuronico Con Enlace Cruzado ZenfilAuthor for correspondence