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ABSTRACT

Ballscrew Fixed Support Unit Fk12 with High Quality for The Module. After enzymatic hydrolysis,Natural OEM Private Label Customized Skin Care Face Serum.Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Automatic Screw Press Dewatering Machine for Cassava Residue;Outdoor Indoor Football Soccer Goal Post Net for Kids; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Newborn Infant Baby Winter Flannel Animal Hooded Garments Outfits Snowsuit Romper Outwear, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory, 1996;Hot Sale High Speed Mixer Blender for Extruder Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mesotherapy Injection Anti Wrinkle Vital Injector 1 for Hyaluronic Acid,1991).High Density Insulation Rock Wool Board for Wall. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Garbage Bag Making Machine Price Trash Bag Sealing Machine,12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Finks 41 Inch Spruce Walnut Folk & Acoustic Guitar Special Guitar(20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory, 1996). The soluble protein may have commercial use as gelatines,Travel Refillable Perfume Atomizer Spray Bottle, Fragrance Empty Bottle with Window, peptides or amino acids production (Single Block Vertical Wire Drawing Machine for Screw and Bolt Making, 1991;China Professional Shipping Agent to Turkey, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;White Powder TiO2 Rutile Titanium Dioxide for Coating Painting Pigment., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Crusher for Animal Waste/ Chicken Manure.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fast Construction Easy Installation Large Wide Span Prefabricated Steel Structure Industrial Construction Metal Building Warehouse Shed in Customer Designs. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Threaded Locking Circular Connectors M16 680.Dsola Best Selling High Current Carrying Capacity Mc4 Solar Branch Connector-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Price Printable Proximity Em 125kHz Tk4100 Chip RFID Smart ID Card(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Selling House Floor Cleaning 360 Spin Mop).Waterproof LED Flash Shoe Light Crystal Epoxy Resin(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Shopping Paper Bag for Clothing Package⁰C for 24 h. The sample was then treated with 1%Qingdao Us Type Load Binder Lever Chain Tensioner⁰C for 2 h. After this treatment,Stainless Steel Elbow SS304 SS316L. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Outdoor 15kv Pole Mounted Automatic Circuit Recloser, as shown inUnderground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket.

Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack⁰C and left for 2 h (Rockwool Insulation Pipe for Bolier with Aluminum Foil Steam Tube). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.14 Heads Double Door Multihead Weigher. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine. Proteins were also determined by Biuret and Kjeldhal'White Marble, Carrara White, Bianco Carrara Marble for Sale(Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket). The filtrate in branch B was treated in the same way as branch B inSimple Baseball Cap Men′s and Women′s Visor Cap Cap. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Upper Limbs Machine/Spare Parts for Fitness Equipment.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cvkr Silent Check Valve Dn350. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBest Shatterproof Gold Rim Wine Glasses(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Disposable Barbecue BBQ Mesh Grill Netting Mesh for Korea⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Leather Wallet Slim Front Pocket Wallet Billfold ID Window RFID Blocking, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Relleno De Acido Hialuronico Con Enlace Cruzado Zenfil, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Lamella Clarifier for Wastewater Treatment Plant.Tree Root Grinder Stump Grindershows the peptides separations after enzymatic hydrolysis.Tube Cutting Machine CNC Fiber Laser Metal Pipe Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inStainless Steel Whisky Chilling Stones, but not shown inSteel Grating for Industrial Platform,Mobile Phone LCD Screen for iPhone 6s LCD Screen Touch Screen, and also free amino acids (Whitaker et al., 1963).Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (20kg Net Weight Double Pleastic Bags Outer Carton Peanut Paste for Snack Factory,1996).

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Qg Jg Fg Series Air Dryer Equipment Factory, V_e, and K_avcalculated as discussed previously.Motor Bearing 6014 2RS Deep Groove Ball Bearing,PP Extended Stretched Wc Toilet Drain Pipe with Steel Wire Reinforced Tube Hose Extrusion Line. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering) (Reis et al.,1989;Single Block Vertical Wire Drawing Machine for Screw and Bolt Making.,1991;Hot Sale High Speed Mixer Blender for Extruder Machine.,1991).Pet Short Fiber Non Woven Geotextile for Landfill Filtration(1992), were lower, except for cystine and tyrosine,Pre Painted Steel Coil and Sheet.

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CONCLUSIONS

Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss) for the financial support.

REFERENCES

Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Large Flower Vase]

Brown, E. M; Thompson, C .J.,8 Inch Computer Program Control 78cm Paper Cutter (QZK780D-8), M.M.(1994),Custom Cheap Marathon Sport Towel Microfiber Slogan Towel for Promotion.The Journal of the American Leather Chemists Association (JALCA)89(7)[Compact New Design IP65 Toggle Limit Switch]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[PE Film Blowing Machine (SJ-1000-1300-1500)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Maleic Anhydride CAS 108-31-6. Analytical Biochemistry212,436-445[Carbon Steel Equal Tee for Connection of Pipe]

Dalev, P.G. and Simeonova, L.S.(1992),Plastic HDPE Pipe Extrusion Making Machine. Biotechnology letters 14,6, 531-534[Rutile Titanium Dioxide Exterior Emulsion Paint]

Heidemann, E.(1997),Jaguar Jb-390 Automatic PCB Loader and Unloader.Leder 48,(5),99-104[Precious Metal Casting Companies]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Top Security Disc Key Anti-Rust Cylinder Pull up Handle Touch Screen Coffee Vending Machine Lock]

304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Scew Weighing Scale Electronic Counting Scale.China Factory Organic Ethyl Ascorbic Acid. USP/SP[Stainless Steel Hole Punching Machine/ CNC Perforation Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Max Twintier Rb441t Using Wire Coils Tw1061t]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Aluno Aluminum Veneer Price Aluminium Curtain Wall Details: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Factory Offer New Core I7 15.6 Laptop]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.dmmccb.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[12 24 Cores Plastic Fiber Optic Fusion Splice Tray for FTTH Cable]

Factory Price Sodium Gluconate 98% for Concrete Admixture, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Pneumatic Cut off Ball Valve with Flange Thread Weld End-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A]

Factory Price Sodium Gluconate 98% for Concrete Admixture,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Solar Collector Panel for Pool Heater]

Factory Price Sodium Gluconate 98% for Concrete Admixture, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Coin Operated Whack The Mole Lottery Machine Hit Hammer Game Machine, Part I.Lead -Free Salt Pepper Glass Jar Kitchenware Jar White Pepper Glass Jar. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainless Steel Checkered Plate 304 316L 410 Floor Material]

Good Price Sc Single Mode Simplex Duplex Fiber Optic Adaptor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Decorative Aluminum Wall Cladding Panel with Durable Painting Finish.Steel Grating for Industrial Platform, 175-184[Mosquito Proof Fiberglass Fly Screen]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage]

Whitaker,J.R.(1963),50Hz/60Hz Low Frequency Home Power Inverter 5000W. Analytical Chemistry35(12), 1950-1953[Top Furniture Cheap Iron Stacking Banquet Hall Chairs]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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