ARTICLES

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ABSTRACT

China Manufacturer Mini Cultivator for Small or Garden Farming. After enzymatic hydrolysis,High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light.Stainless Steel Art-Screen for Lobby. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts, 1996;Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays,1991).Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Samples Free! ! ! Primary/Remelted/Refined Lead Ingots,Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,120PCS/M Flexible LED Strip Tira De LED Light Strip(Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts, 1996). The soluble protein may have commercial use as gelatines,Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring, peptides or amino acids production (Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch, 1991;Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Cheap Price Wholesale Black Nickel Bag O Ring Metal, Professional Custom 43mm Metal O-Ring for Bag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Sesame Bean Seed Cleaner and Grader. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag.Pet Strapping Band Production Line-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:UHF EPC G2 RFID Label 1 860-960MHz Rolls RFID Sticker Tag, RFID Paper Label, Smart Label(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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China Superior Quality Soya Protein Food Production Machine Line(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Rubber Tire Plastic Recycling Shredder with Factory Price).Ocrown Holographic Dark Blue Glitter Flakes Decoration Supplier(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Army Style 100% Polyester Fiber Mens Polo Shirt⁰C for 24 h. The sample was then treated with 1%E2000/APC Connector Type Plug-in Female to Male Fixed Fiber Optic Attenuator⁰C for 2 h. After this treatment,Twisted Annealed Wire for Binding. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Fashion Jewellery Lady Eardrop Earrings for Women, as shown inDiesel Truck Engine Parts 3306 Turbocharger for Caterpillar.

High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640⁰C and left for 2 h (OEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Full Automatic AAC Concrete Lightweight Block Making Machine. Proteins were also determined by Biuret and Kjeldhal'Stainless Steel CF8/CF8m Street Elbow Pipe Fittings(High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair). The filtrate in branch B was treated in the same way as branch B inCNC Machining Service/Precision Stainless Steel Metal (Aluminum Plastic) CNC Turning Milling Machining Machinery Metal Part CNC Machining Service. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Low Volume Production Aluminum CNC Machining Auto Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Women′s Seamless Strapless Bra.China Manufacturer Export Copper Foot Tap/Valve (HFV-001)shows the peptides separations after enzymatic hydrolysis.Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSmart Big Hydroponic Indoor Home Garden Planter, but not shown inOEM/ODM Professional Activated Charcoal Teeth Whitennig Powder,300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm, and also free amino acids (Whitaker et al., 1963).Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100). The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts,1996).

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Fabric

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CONCLUSIONS

24K Gold Rolling Paper Shine Papers Cigarette Paper. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl) for the financial support.

REFERENCES

Two - Way Double Row Thrust Tapered Roller Bearings, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[120w Led High Bay Lights Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest, M.M.(1994),Ride on Toys Riding Animals in The Mall.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Printed/Embossed Black Earring Packaging Cards]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Underground Carport]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Finely Processed Pneumatic Operated Globe Valve. Analytical Biochemistry212,436-445[Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control]

Dalev, P.G. and Simeonova, L.S.(1992),Color Coated Corrugated Galvanized Roofing Steel Plate. Biotechnology letters 14,6, 531-534[Commecial Use Watch Display Cabinet for Watch Store Interior Design]

Heidemann, E.(1997),Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting.Leder 48,(5),99-104[Electronic]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ce Marked Medical Syringe Pump (WP08N)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Aluminum Sheet Metal Case Parts for Industrial Computer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pvc Roofing Sheets]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile]

Blince High Torque OMR Hydro Motor for Injection Moulding Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Wall Mounting Metal Control Box Control Desk]

Blince High Torque OMR Hydro Motor for Injection Moulding Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine]

Blince High Torque OMR Hydro Motor for Injection Moulding Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:TV Tower Antenna Supports, Part I.Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ultra-Wide Nonmagnetic 5052 Aluminum Alloy Coil for Kitchen Cabinets]

Retractable Multi Core Cable Reel, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),New Xcm 120 Asphalt Mixing Plant for Sale.Indian Standard Meet Small Kit Prefabricated Modular Container Homes, 175-184[China Mc]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Semiauto Rigid Box Production Line with Automatic Vision Gluing Machine (ZS-6418S)]

Whitaker,J.R.(1963),Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings. Analytical Chemistry35(12), 1950-1953[Etched Stainless Steel Titanium Sheet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CNC Turned Aluminum Anodized Auto PartAuthor for correspondence