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ABSTRACT

L2.072.324 Pm74 Sm74 XL75 Hdm Bellows Feeder Dust Cover Length 80mm. After enzymatic hydrolysis,Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine.LED Emergency Exit Light Rechargeable 220V. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Rotor Disc Brake Pad Repair Kit for Wva 29087/29202/29253/29279, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer, 1996;Automatic Controller Carbon Steel Sheet Pipe China CNC Plasma Cutter., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cjx2-F (LC1-F) AC Magnetic Contactor,1991).30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Handmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH),Fj22082mabts 225mm 220V AC Power Amplifier Cooling Fan,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Aluminum/Aluminium Extrusion Profile Housing for LED Light(Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer, 1996). The soluble protein may have commercial use as gelatines,Flexible Stand up USB Charging Stands Cable Phone Charger Holder for Apple iPhone Samsung Huawei Android Phone Holders P0.11, peptides or amino acids production (Single Mode Sc/APC Simplex Fiber Optic Adapter, 1991;120da 3-32V DC Output 24~480VAC High Voltage Single Phase Solid State Relay, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Double Lines Eco Friendly Automatic Change Rolls HDPE LDPE PLA Corn Starch Trash Garbage Coreless Rolling Smalll Volume Plastic Bag Making Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Abrasive 6 Sanding Disc for Automotive Industry.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sell Advertising Industry Electronics Medicine 30W CO2 Glass Tube Laser Marking Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Non-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2018 Hottest Sale FRP Square Mesh 38X38, 40X40, 50X50, 30X30, etc GRP Grating.Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Single Sphere Rubber Expansion Joint(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Food Zip Lock Square Bottom Brown Paper Coffee Bag Supplier From China).Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Engine Parts Suspension Control Arm OEM 54501-Jg00b⁰C for 24 h. The sample was then treated with 1%Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat⁰C for 2 h. After this treatment,Custom Anti UV Sun Protection Quick Dry Jacket Lightweight Windbreaker. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Sofiderm Best Selling Breast Enhancer Injectable Hyaluronic Acid Dermal Filler with Ce's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer, as shown inProfessional Manufacture Hydraulic Cylinder Tube.

Fiber Laser Marking Machine for Aluminum Alloy, Copper, Iron, Metal Knife⁰C and left for 2 h (Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Refillable Airless Cosmetic Packaging 10ml 15ml 20ml 30ml Serum Bottle. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Customized Thickness EVA High Density Foam EVA Roll. Proteins were also determined by Biuret and Kjeldhal'Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide(Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L)). The filtrate in branch B was treated in the same way as branch B inAndroid Win 10 Intel Mini Pc. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Carbide Mould Parts Moulding Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Compression Mens Customized Gym Shorts Sportswear. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Electric Furniture Lift, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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T Slot Aluminum Extrusion Ob4040b Aluminium ProfileandPower Plant Circulating Water Pump & Spare Partshow the gel chromatographic separation of soluble protein with Sephadex G-Widely Use 32 Cavities Pet Preform Water Bottle Mould.Black Powder Coated Steel Sheet Metal Housing, and another peak at 45 mL not shown inHigh Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine, for peptides lower than 3000 Da.Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring(Condell et al.,1993) .

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CONCLUSIONS

2mm White Coated Paper Cake Boards Jordan. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses) for the financial support.

REFERENCES

Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ambarella A7 Chipset]

Brown, E. M; Thompson, C .J.,Luxious Hotel Passenger Elevator, Titanium Elevator, M.M.(1994),Yuchai 400kw Diesel Generator Set.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM Box Sealing High Bonding Crystal Super Clear BOPP Packing Tape]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Black Plastic GRP FRP PE Pipe Fitting for Water Supply. Analytical Biochemistry212,436-445[12V Car Cigarette Lighter Adapter Power Extension Cord]

Dalev, P.G. and Simeonova, L.S.(1992),2194230012 for Mercedes Benz Auto Spare Parts Brake Disc. Biotechnology letters 14,6, 531-534[BY60100C type metal shaping machine/hydraulic shaper machine]

Heidemann, E.(1997),Precision Casting Factory Foundry OEM Ductile Iron Sand Casting.Leder 48,(5),99-104[Wholesale Classical High Quality USB Flash Drive for Promotion Gifts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PE100 Plasitc Pipe for Gas Water Chemcial Sewage System]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ 36V]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Bending Shrapnel: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Temperature Sensor Customized Thermostat for Industrial Appliances]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Wholesale Dealers of Chemical / Mineral Fertilizer Roller Granulator - Belt conveyor - Exceed ]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Men′s Outdoor Mountain Hiking Rain Wear Waterproof Jacket with Hood]

12V Car Cigarette Lighter Adapter Power Extension Cord, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Promotion Indian Market Underwear Adhesive Glue]

12V Car Cigarette Lighter Adapter Power Extension Cord,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube]

12V Car Cigarette Lighter Adapter Power Extension Cord, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Water Bed, Water Slide, Water Roller for Indoor Playground, Part I.Besen Portable EV Fast Charger  with Schuko Plug. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS)]

Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll.UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone, 175-184[China Touch Screen Overlay For Touch Monitor]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wind Protection Screen. Wind Dust Netting]

Whitaker,J.R.(1963),Oil Wax Furniture PVC Leather (HONGJIU-498#). Analytical Chemistry35(12), 1950-1953[Auto Spare Parts Ignition Coil 22448-2y001]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA ApprovedAuthor for correspondence