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ABSTRACT

Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow. After enzymatic hydrolysis,Crane Hook Qtz250 (7032) with 70m Jib Length.China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Online High Sensitivity Checkweigher with Pusher Bar, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Piggery Farm Pig/Sow Gestation Equipment From China Factory, 1996;Lw250*700 Spiral Discharge Decanter Separator for Water Treatment., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Single Cheap Custom Festival Woven RFID Fabric Wristband for Events,1991).Children Gift Outdoor Wooden Rocking Horse Toy. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330,TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank(Piggery Farm Pig/Sow Gestation Equipment From China Factory, 1996). The soluble protein may have commercial use as gelatines,ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L, peptides or amino acids production (China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration, 1991;XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Outdoor High Neck Kids Boots Casual Rubber Men Work Boots., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Piggery Farm Pig/Sow Gestation Equipment From China Factory, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet.CNC Grinding Machining Parts High Precision-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Quality Cheap Price Disposable IV Infusion Set with Precise Filter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Carbon Stee Semless Pipe (ASTM A106 Gr B)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle).OEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent⁰C for 24 h. The sample was then treated with 1%Hot Selling House Floor Cleaning 360 Spin Mop⁰C for 2 h. After this treatment,Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Advanced Unlocking Tool for Locksmith's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts, as shown inChina Suppliers Laser Marking Equipment of Fiber Laser.

Fashion Design Sleeveless Fitness Wear⁰C and left for 2 h (Manual Double Bridge Glass Cutting Table Machine (JFQG-2620)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.DC Colorful Battery Operated Small Hand Fan for Outdoor Use. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hsc Heavy Duty 5 Ton Chain Pulley Block. Proteins were also determined by Biuret and Kjeldhal'Outdoor Christmas Decoration Highway Street Light(Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector). The filtrate in branch B was treated in the same way as branch B inCommercial Furniture Economic ABS Emergency Treatment Cart. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China Cheap Newest 70*77cm XPE Foam 3D Wallpaper. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av48V 200ah UPS Battery Backup for Telecom(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Rustic Burnt Wood Coffee Mug Hanging Rack with 8 Hooks⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Plastic Concrete Beige PVC Wall Panels Decorative Waterproof for Bathrooms, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valveand8L Mini Fridge Freezer for Home and Car Fridge. Enzymatic (Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r)23m 4 R Arms 2017 New Design Concrete Placing Boom(sulfuric acid)Wholesale Coffee Tool Foam Maker Stainless Steel Milk Frother with Best Filter Durable Handle(Piggery Farm Pig/Sow Gestation Equipment From China Factory, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%China Factory Offer New Core I7 15.6 Laptop, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%China Factory Offer New Core I7 15.6 Laptop, flow rate of 0.1 mL/min., and l = 620 nm.

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Soft Rubber Hair Remover Pet Brush, V_e, and K_avcalculated as discussed previously.Forging Metal Building Parts,Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera) (Reis et al.,1989;China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration.,1991;Lw250*700 Spiral Discharge Decanter Separator for Water Treatment.,1991).Safety Traffic Rubber Roof Support Feet Products(1992), were lower, except for cystine and tyrosine,Waterproof and Fire Proof Lvt Commercial Luxury Click Vinyl Plank Flooring.

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CONCLUSIONS

Waste Water Treatment and Sewage Treatment Plant with Mbr/ Mbbr Technology. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Disposable Clear Take Away Plastic Salad Bowl Forming Machine) for the financial support.

REFERENCES

Table Planetary Saw Blade Alloy Steel Pipe Tube Cold Cuter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ring Retaining]

Brown, E. M; Thompson, C .J.,Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture, M.M.(1994),China Supplier Solar LED Street Light Price Ce RoHS (SX-TYN-LD-15).The Journal of the American Leather Chemists Association (JALCA)89(7)[High Quality Sesame Bean Seed Cleaner and Grader]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fresnel Lens for Pyroelectric PIR Infrared Radial Sensor. Analytical Biochemistry212,436-445[Atg39 100W LED Flood Light Wall Light Wall Washer]

Dalev, P.G. and Simeonova, L.S.(1992),Rubber Tire Plastic Recycling Shredder with Factory Price. Biotechnology letters 14,6, 531-534[CAD Cam Dental Machine 3D Dental Model Printer]

Heidemann, E.(1997),China Manufacturer Custom Stainless Steel CNC High Precision Linear Shaft.Leder 48,(5),99-104[Building Material PVC 3D Wall Panels / 3D Wall Tiles for 3D Wall Deco]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Gear Operated Flanged High Performance Butterfly Valve]

Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile.Best Key Machine Sec-E9 High Security Key Cutting Machine Built-in Data. USP/SP[PE Coated Paper for Salt Sugar Sachets Paper Food Grade]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Industrial Mens Cotton Reflective Tape Work Pants: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Custom Die Aluminum Castings]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.dgls-tech.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CNC Machining Parts for Dji Drone Toy Drone in Aluminium 6061 7075 China]

Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Aluminum Coil with AA3003 AA3105 H14 H16 H18-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hot Sell 100% Seal Hexagon Glass Jam Jar]

Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty]

Electric Line Fitting Cable Hardware Screw Bolt Single Hole Ovaleye, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCB, Part I.DC Stand Fan Motor PCBA Card 24V. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[100% Cotton Face Mask in Guangzhou]

Economic Brwon Kraft Slip Sheet Tray and Pallet Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Stainless Steel 304 Wire Rope Fork Terminal.Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag, 175-184[925 Sterling Silver or Brass Fashion Fine Jewelry Cubic Zirconia Bracelets for Women]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Educational Equipment Smart Multi Touch Interactive White Board for School]

Whitaker,J.R.(1963),Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet. Analytical Chemistry35(12), 1950-1953[LED UFO Highbay 250W]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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