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ABSTRACT

Customized Thickness EVA High Density Foam EVA Roll. After enzymatic hydrolysis,Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide.Carbide Mould Parts Moulding Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder, 1996;P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Electric Furniture Lift,1991).Roller Shutter Slat and Panel Manufacturer and Complete Roller Shutter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet,Construction Material Hoist Rack and Pinion,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3000lb Small Boat Marine Electric Trailer Winch Power-in, Power-out(Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder, 1996). The soluble protein may have commercial use as gelatines,Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks, peptides or amino acids production (Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal, 1991;2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Quality Endoscopic Surgical Adult Disposable Bite Block., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China OEM ABS Clear Plastic Injection Molding.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Widely Use 32 Cavities Pet Preform Water Bottle Mould. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Black Powder Coated Steel Sheet Metal Housing.Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hot Sell 100% Seal Hexagon Glass Jam Jar(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

2mm White Coated Paper Cake Boards Jordan, Parana State,Refillable Airless Cosmetic Packaging 10ml 15ml 20ml 30ml Serum Bottle.Multiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the13.8cm Reed Diffuser Clear Glass Bottle for AromaandDry & Wet Concrete Surface Metal Grinding Wing.

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Luxious Hotel Passenger Elevator, Titanium Elevator(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Luxury Plastic Pump Cosmetic Packaging as Airless Bottle).Yuchai 400kw Diesel Generator Set(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Black Plastic GRP FRP PE Pipe Fitting for Water Supply⁰C for 24 h. The sample was then treated with 1%2194230012 for Mercedes Benz Auto Spare Parts Brake Disc⁰C for 2 h. After this treatment,Precision Casting Factory Foundry OEM Ductile Iron Sand Casting. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Sale Electrical Motors Small for Home Appliance's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line, as shown inMechanical Stage Collar Cementing Tool Hydraulic Stage Cementing Tool.

Bending Shrapnel⁰C and left for 2 h (I12 Bluetooth 5.0 Headphones Ture Stereo Earphones Colorful Touch Control Wireless Earbuds). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.12V Car Cigarette Lighter Adapter Power Extension Cord. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals. Proteins were also determined by Biuret and Kjeldhal'Water Bed, Water Slide, Water Roller for Indoor Playground( 400A). The filtrate in branch B was treated in the same way as branch B inGa11 Compressor Replacement Parts Unloader Intake Valve 2901029850 Repair Kits. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Besen Portable EV Fast Charger  with Schuko Plug.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Oil Wax Furniture PVC Leather (HONGJIU-498#)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Automatic Bagging Liquid Sauce Sachet Water Filling Sealing MachineandChina High Efficiency Automatic Glue Dispensing Machine with Factory Price. Enzymatic (CNC Machining Mechanical Parts Fabrication Service)Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch(sulfuric acid)Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall(Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Electronic Pulse Gas Stove Burner Igniter Transformer Parts, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Electronic Pulse Gas Stove Burner Igniter Transformer Parts, flow rate of 0.1 mL/min., and l = 620 nm.

CNC Turned Aluminum Anodized Auto Part.SGS 40L Lab Use Chocolate Refiner Conching Machineshows the peptides separations after enzymatic hydrolysis.Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCarbon Stee Semless Pipe (ASTM A106 Gr B), but not shown inNatural Marble Stone Carving Granite Water Garden Decorative Statue Fountain,Commercial Vegetable Mushroom Coconut Meat Drying Machine, and also free amino acids (Whitaker et al., 1963).4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder,1996).

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CONCLUSIONS

White and Green Board, School Writing Board, School Furniture (GT-76C). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape) for the financial support.

REFERENCES

Current Precise LED Digital Ammeter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Antique Brass Towel Ring]

Brown, E. M; Thompson, C .J.,Automatic Drinking Mineral Water Filling Line/Water Bottling Line, M.M.(1994),Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men.The Journal of the American Leather Chemists Association (JALCA)89(7)[Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-PP Polypropylene Two Layer Film Blowing Machine. Analytical Biochemistry212,436-445[Weaving Poly Wicker Cookeware Gas Fireplace Rattan Fire Pit Table Set Furniture]

Dalev, P.G. and Simeonova, L.S.(1992),Disposable Sugarcane Pulp Paper Bowl 500ml. Biotechnology letters 14,6, 531-534[Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible]

Heidemann, E.(1997),Flat Roof Insulation Board Heat Insulation Board.Leder 48,(5),99-104[www.sheenlandglass.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[2.5X/3.5X Glasses Clip Dental Loupes Binocular Surgical Loupe Lab Medical Magnifier]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Eco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Wholesale Soft Hallux Valgus Bunion Corrector Brace for Sport Protection: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Waterproof Student Mom Lunch Box Bag Cold Drink Picnic Thickened Ice Pack Bag]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.cryspak.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Stainless Steel Seamless Coil Tube ASTM A249/A269]

Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Solar Power Panels Kits Modules Home Solar System USB Fan Bluetooth Music Speaker-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit]

Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Blue Color Fish Paper/6520/6521 Flexible Composites]

Automatic Bagging Liquid Sauce Sachet Water Filling Sealing Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:304 Stainless Steel Chocolate Pump for Delivering Chocolate Paste, Part I.Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cattle Feed Factory Used Dust Collector and Filter]

8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Ballscrew Fixed Support Unit Fk12 with High Quality for The Module.Natural OEM Private Label Customized Skin Care Face Serum, 175-184[Forged Carbon Steel Welding Neck Stainless Steel Flange]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[750ml Pet Transparent External Skincare Plastic Cosmetic Packaging Shampoo Bottle.]

Whitaker,J.R.(1963),Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering. Analytical Chemistry35(12), 1950-1953[New Long Life Petrol Gas Wood Cutting Machine Hand Manual Garden Field Steel Power Tools CS4500 Gasoline Chain Saw]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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