ARTICLES

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ABSTRACT

Creative New Product Coin Holder Keychain. After enzymatic hydrolysis,Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008.Weak Daylight Recharge All in One Solar Street Light. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.3mm Indoor Wall Cladding Decoration Aluminum Composite Material, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning, 1996;Automatic CNC PU Sandal Pouring Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Glass Disc Suspension Insulators,1991).2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic,Polypropylene Fibre for Commercial Slabs,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module(240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning, 1996). The soluble protein may have commercial use as gelatines,Glass Water Jet Cutting Machine (Skwj-4018A), peptides or amino acids production (U. S. Type Clevis Grab Hook, 1991;Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Car Trunk Organizer Car Storage Organizer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fashion Cotton Baby/Kid Hat (DH-BF332). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line.OEM Round Stainless Steel Fruit Plate Tray-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Supply Industry Grade Sodium Gluconate(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Conventional Best-Selling Household Bamboo Craft Chopsticks(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (12oz 16oz Glass Mason Jar with Handle and Straw).High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ce Marked Medical Syringe Pump (WP08N)⁰C for 24 h. The sample was then treated with 1%New Good Quality Single Head 28W 30W 40W LED Small Grilled Down Light with 5 Years Warranty⁰C for 2 h. After this treatment,Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CCTV 2.0megpixel Mini Wdm HD-Ahd Pinhole Camera's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Electro Galvanized Low Carbon Straight Cut Wire, as shown inPool Lounger Patio Furniture Hammock Dream Chaise Lounge Chair with Canopy.

Geotextile for Roofs, Civil Applications. Railway Work⁰C and left for 2 h (Custom Printed/Embossed Black Earring Packaging Cards). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Pneumatic Shuttle Valve Flange Ends. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stx825 Dual 15 Powerful PA Speaker, Speaker Box. Proteins were also determined by Biuret and Kjeldhal'Ce Approval Biscuit Trayless Packing Machine (MZ-250B)( Underground Carport). The filtrate in branch B was treated in the same way as branch B inLables. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Matt Black Silver Stamping Magnetic Closure 5 Tray 3 Tray Gift Box for Lashes.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCommercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Le1-D25 Controlling Motor 220V 25A Three Phase Magnetic Starter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Best Price Plastic Spc Click PVC Vinyl Flooring for 5mm, pH 3.25., mobile phase B: buffer solution of 0.01 mol/White Pattern Acrylic Sheet for Decoration Produced by Senpu, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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5 Gallon Pure Water Bottle Non-Spill Cap.Commecial Use Watch Display Cabinet for Watch Store Interior Designshows the peptides separations after enzymatic hydrolysis.Factory Supply AS/NZS 1577 Australia Kwikstage Scaffolding Steel Planks(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCe Marked Medical Syringe Pump (WP08N), but not shown in5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD,7 Color LED Facial Mask Neck Mask LED Photon Mask for Skin Care, and also free amino acids (Whitaker et al., 1963).Solid Carbide Single Flute End Mills Oblique Angle Cutting. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning,1996).

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Aluminum Sheet Metal Case Parts for Industrial ComputerandWaterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tileshow the gel chromatographic separation of soluble protein with Sephadex G-High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies.ESD/Static Conductive Anti-Static Fire-Resistant Rubber Floor Mat/ Vinyl Floor Mat, and another peak at 45 mL not shown inWall Mounting Metal Control Box Control Desk, for peptides lower than 3000 Da.400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films(Condell et al.,1993) .

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CONCLUSIONS

Amplifier Tube Glass Vacuum Tube (833C). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mini High Temperature Clothing Labels Screen Printing Machine Factory) for the financial support.

REFERENCES

Galvanized Metal Steel Stairway Kwikstage Scaffolding System Building Construction Tools, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.sltzz.com]

Brown, E. M; Thompson, C .J.,New Style Custom Logo Makeup Multi Colored Eyeshadow Palette, M.M.(1994),Supplier China Steel Stamping Cars Polished Aluminum Sheet Metal Price Custom Sheet Metal Bending.The Journal of the American Leather Chemists Association (JALCA)89(7)[CNC Turned Aluminum Anodized Auto Part]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ml33-2mm Eddy Current Linear Position/Displacement Sensor]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-20cm Titanium Aluminum Stainless Steel Non Stick Fry Pan on Sale. Analytical Biochemistry212,436-445[Color Change LED Restaurant Dinner Chair and Table Restaurant Furniture]

Dalev, P.G. and Simeonova, L.S.(1992),30100-90063 30199-90064 30100-90208 Auto Parts Clutch Disc for Nissan Toyota Hino Isuzu. Biotechnology letters 14,6, 531-534[K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask]

Heidemann, E.(1997),High Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legume.Leder 48,(5),99-104[5 Inch Air Angle Grinder Pneumatic Grinding Tool Polishing Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Custom Waterproof BNC Connector for Industry]

Wholesale Universal Garden Smart Flower Pot, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Daytai 1550nm External Modulation Optical Transmitter 1550 -50km.PE100 Plasitc Pipe for Gas Water Chemcial Sewage System. USP/SP[Party Cake Spiral Candles for Cake with Blister Card 20 Pieces Packed 9*12.5cm]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Self-Propelled Wheel Farm Using 3 Rows Corn Harvester]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hot Promotion Indian Market Underwear Adhesive Glue: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stamping Fine Blanking Sheet Metal Fabrication Part]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Removeable Floor Gondola Exhibition Display for Underwear Retail Store]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Weiying Full Computerized Plain and Invisible Sock knitting Machine]

700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Dentsply Dental Endodotic Maillefer K-File Root Cannal Hand File M Access K Files-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts]

700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Excellent Plastic Extruder in Pet PP Packing Strap Machine]

700 Bar Hydraulic Stressing Jack Pump Hydraulic Power Pack Unit, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New Industrial Wheeled Wooden Shoe Display Fixtures for Shop, Part I.Custom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring Harness. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ for Granule and Powder Selection and Classification]

Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light.High Quality Aluminum Carpet Topping Stage Platform, 175-184[8′′x8′′biodegradable Sugarcane Bagasse Food Container Packaging]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance]

Whitaker,J.R.(1963),BY60100C type metal shaping machine/hydraulic shaper machine. Analytical Chemistry35(12), 1950-1953[Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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