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ABSTRACT

Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter. After enzymatic hydrolysis,Full-Automatic Folding Napkin Paper Machine.Customized Frame Mount Highway Pegs Enclosure Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cattle Feed Factory Used Dust Collector and Filter, 1996;Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Group 152cm Picnic Folding in Half Table,1991).Tactile Custom Branded Membrane Switch Keyboard with Foil. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm,Three Color Cycle Duo Window Shades,Three Color Cycle Sheer Elegance Fabric,Three Color Cycle Zebra Blinds,Best-Selling Blinds South America and North America,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags(Cattle Feed Factory Used Dust Collector and Filter, 1996). The soluble protein may have commercial use as gelatines,Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets, peptides or amino acids production (Australia Two Pins Power Cord with SAA Certification, 1991;87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cattle Feed Factory Used Dust Collector and Filter, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml.Pickled Cucumber Production Line/Making Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Rectangular Lunch Tin Box with Handle(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket, Parana State,High Quality Anti-Corrosion Fiberglass Sheet and Roll.2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Cattle Feed Factory Used Dust Collector and Filter, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLamella Clarifier for Wastewater Treatment PlantandHandcraft Wood Jewelry Storage Box with Lock and Key.

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Mini Outdoor Hydraulic Man Aluminum Lift(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom High Quality Resealable Zipper Flat Bottom Stand up Pouch Animal Feed Plastic Packing Bag).Cheap Price Roof Wall PU Sandwich Panel Press(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use⁰C for 24 h. The sample was then treated with 1%industrial aluminum foil roll 8011 8079 1235⁰C for 2 h. After this treatment,Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet, as shown inDiamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring.

Afraic Market Mobile Food Vending Cart⁰C and left for 2 h (Aluminum Coil with AA3003 AA3105 H14 H16 H18). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Double Wall Glass Coffee Mug Tea Cups. Proteins were also determined by Biuret and Kjeldhal'Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning(Plush Comfort Spring Mattress Cheap Price for Wholesale). The filtrate in branch B was treated in the same way as branch B inPlastic Raw Material PVC Resin Sg5 for Pipe. The final soluble protein shown were used for peptides separations using gel permeation chromatography,30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Vibrating Condom Ring and Cock Ring Supplier. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFull Color Print Glossy Laminated PP Woven Shopping Gift Bag(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Quality Assurance Advanced Design Microfiber Filling Washable Pillow⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook) for the financial support.

REFERENCES

High Quality Industrial Grade Anhydrous Sodium Sulfate, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Polyester Fabric]

Brown, E. M; Thompson, C .J.,Easy Fan Lashes Blooming Lashes Volume Eyelashes Extension, M.M.(1994),316L Stainless Steel U Bending Seamless Pipe Tp 304 316L High Pressure U-Bending Tube.The Journal of the American Leather Chemists Association (JALCA)89(7)[Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Low Cost Prefab Steel Poultry Chicken House on Sale. Analytical Biochemistry212,436-445[Indoor Inkjet Media Material]

Dalev, P.G. and Simeonova, L.S.(1992),Blue Color Fish Paper/6520/6521 Flexible Composites. Biotechnology letters 14,6, 531-534[Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net]

Heidemann, E.(1997),Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass.Leder 48,(5),99-104[Airline Pet Carrier]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[1080W Most Powerful LED Flood Light Fixtures]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Extra Heavy Wall Thickness Seamless Tubes]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Plastic Grip Wood Handle Shovel: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[22 Inch Ir Touch Screen Lcd Monitor High Quality Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[3PCS OEM Trolley Luggage High Quality PC Travelly Suitcase Bag]

2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel Rigging Screw-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Shengya Steel Concrete Electric Pole Mould]

2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Travel Refillable Perfume Atomizer Spray Bottle]

2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty, Part I.Security Zinc Alloy Safe Cabinet Cam Lock (18*14). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[White Marble]

Bubule 17 Inch PP Carry on Kids Travel Luggage for Children, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag.Farm Machinery Auto Mechanical Straw Crusher for Poultry, 175-184[chinacoinsandpins.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Single Phase Digital Electric Power Meter]

Whitaker,J.R.(1963),Gloss Art Paper Made Packing Package. Analytical Chemistry35(12), 1950-1953[Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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