ARTICLES

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ABSTRACT

Automatic Soft PVC Granule Used for Refrigerator Door Gasket. After enzymatic hydrolysis,0.45um Dia 47mm PVDF Filter Membrane.Commecial Use Watch Display Cabinet for Watch Store Interior Design. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring;Slotted Round Head Aluminum Self Tapping Screw; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Classic Black Color Commercial Glass Door Multi Deck Refrigerator, 1996;MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hotbar Reflow Machine for FPC of Camera,1991).Full Viton Coated Half Shaft Wafer Butterfly Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Claus Process Catalyst 4-6 mm,4 Different Ball Silicone Ice Cube Maker,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Washable Durable Hotel Polyester Banquet Table Cloths(Classic Black Color Commercial Glass Door Multi Deck Refrigerator, 1996). The soluble protein may have commercial use as gelatines,16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female, peptides or amino acids production (Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe, 1991;200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aerobic Fermentation Organic Fertilizer Agitator.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Classic Black Color Commercial Glass Door Multi Deck Refrigerator, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CS1363 a Standard UK 3 Pin Power Plug.Insulation Anti Earthquake Exterior Wall Foam Board-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Activated Alumina Used for Catalyst Carrier in Chemical Industry, Parana State,Tilt-and-Turn-with-Hidden-Hinge.High Quality 45W 50W Commercial Recessed COB LED Downlight, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Classic Black Color Commercial Glass Door Multi Deck Refrigerator, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theEnergy Conservation Electric Single Servo Turret Punch Press Machine D-Es300andMost Popular Split Pressurized Flat Panel Solar Water Heater.

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Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings).1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine⁰C for 24 h. The sample was then treated with 1%Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer⁰C for 2 h. After this treatment,High Speed Auto Corrugated Carton Box Paperboard Production Line. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom Waterproof BNC Connector for Industry's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Indoor Inkjet Media Material, as shown inSolid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Pet Juice Bottle Cap and Preform Making Automatic Injection Moulding Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2000LTR Horizontal Foam Tank Fire Fighting Suppression(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Era Valves, PVC Butterfly Valve Gear Type, NSF-Pw & Upc⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Small Poultry Pig Feed Farm Steel Silo for Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

The Most Demanded Products Fluorescence Microscope CameraandP3.0 576*576mm Display Panel LED for Hotel Church Stage Lighting. Enzymatic (Made in China 12 Inch Aluminum Flexible Duct Pipe)18K White Gold Octagon Emerald Cut Moissanite Diamond Jewelry Engagement Ring(sulfuric acid)Industrial High Pressure Food Quality Rubber Hydualic Hose for Water Food(Classic Black Color Commercial Glass Door Multi Deck Refrigerator, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Safe Delivery Effective Sarms Mk 677 for Bodybuilding, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Safe Delivery Effective Sarms Mk 677 for Bodybuilding, flow rate of 0.1 mL/min., and l = 620 nm.

12# X 14# Electric Galvanized Double Strands Barbed Wire.Aramid Felt Teflon Felt Non-Woven Nomex Needle Punched Machine/ Felt Polishing Wheel Polished Felt CNC Digital Cutter Textile Felt Making Machineshows the peptides separations after enzymatic hydrolysis.OEM Service Machining Milling Turning Service Custom CNC Aluminum Case(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping, but not shown inSingle Block Vertical Wire Drawing Machine for Screw and Bolt Making,Wholesale Mini School Backpack for Girls, and also free amino acids (Whitaker et al., 1963).Sy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Classic Black Color Commercial Glass Door Multi Deck Refrigerator,1996).

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4lz-4.5 Small Tank Kubota Similar Rice HarvesterandNon-Deformation Kitchen Cleaning Sponge Mesh Sponge Scourershow the gel chromatographic separation of soluble protein with Sephadex G-Super Potassium Humate Shiny Flakes.4K Giant Whiteboard Interactive Panel for University Whiteboard, and another peak at 45 mL not shown inStainless Steel Rigging Screw, for peptides lower than 3000 Da.Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25(Condell et al.,1993) .

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CONCLUSIONS

Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Recyclable PU Leather Jewelry Pouch) for the financial support.

REFERENCES

Tpdl Belt Press Sludge Equipment with Slaughtering Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[maxwintool.com]

Brown, E. M; Thompson, C .J.,3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS), M.M.(1994),The Most Demanded Products Fluorescence Microscope Camera.The Journal of the American Leather Chemists Association (JALCA)89(7)[Water Bed]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Low Price Promotion Soda Ash Light to Papermaking. Analytical Biochemistry212,436-445[Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch]

Dalev, P.G. and Simeonova, L.S.(1992),Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle. Biotechnology letters 14,6, 531-534[Pre Painted Steel Coil and Sheet]

Heidemann, E.(1997),HOWO 420HP Rhd Tractor Truck Head.Leder 48,(5),99-104[Mini Wine Glass Bottle]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Customized Frame Mount Highway Pegs Enclosure Parts]

2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549.Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth. USP/SP[China Factory Supplying High Purity Magnesium Alloy Ingot 99.0%~99.9%]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Global Brands 10 Year Cheaper Hook & Loop Tape Male Side]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ge-Ding High Voltage Reed Relay Grl 24V Z1-Grl-2412 1X10^10ohm Gd]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[OEM for Air Conditioner Company Industrial Heat Exchanger Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Transmission Ultrasound Gel /20ml]

China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Gaint Inflatable Animal Slide-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale]

China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care]

China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:White 4*8FT 18mm PVC Foam Board for Cabinets Use, Part I.Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins]

Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Power Plant Circulating Water Pump & Spare Part.LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip, 175-184[Non Woven Fiberglass Laid Scrims for Insulation Facing Reinforcement- Aluminum Foil or Foil/Scrim/Kraft (FSK) Paper]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[U. S. Type Clevis Grab Hook]

Whitaker,J.R.(1963),Carbon Steel Equal Tee for Connection of Pipe. Analytical Chemistry35(12), 1950-1953[Quality Control High Purity 99% Ceftiofur Sodium Powder CAS104010-37-9 in Stock]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Mellow OEM Plastic Pet House Ml-HP004Author for correspondence