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ABSTRACT

Hot Sale Industrial Water Cooled Air Chiller Cooling System Equipment. After enzymatic hydrolysis,Chinese Top Manufacturer Aluminium LED Profile for LED Linear Light.High Quality Aluminum Carpet Topping Stage Platform. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Spplier 70W Solar Street Light with Camera Monitoring System, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud, 1996;Office Supplies Flexible Plastic Writing Clip Board PP Foam Clipboard., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Anti-Counterfeiting Adhesive Seal Tamper Evident Security Warranty Void Tape,1991).Excavated Caterpillar Loader Mining Tunnel Loader Mucking Loader. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush,Easy Installed Microwave Drying Equipment for Fish /Shrimp/Seafood for Small Factory,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Masturbators Adult Sex Toys Japanese Love Doll(Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud, 1996). The soluble protein may have commercial use as gelatines,Cheap 20tons LPG Semi Trailers Gas Tank Transport Trailer for Sale, peptides or amino acids production (Carbon Steel Spherical Plain Radical Bearings, 1991;Electrostatic Powder Color Coating Steel Coil Plate High Quality Z30-Z220 PVDF High Tensile High Strength, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.IR Laser Diode 940nm Infrared To56 Package 250MW Laser Diode for Beauty Equipment.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Powder Spray Booth for Metal Furniture. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Small Tin Box for Condoms.Drive in Rack 2 Ton Reach Warehouse Forklift Battery Electric Reach Truck Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Petrochemical Loading Platform Folding Stairs(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

64mm Double Braid UHMWPE Tugboat Mooring RopeAlcalase2.5 L DX,Rubber Soles Vulcanizing Machine/ Press Machine, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Hot Sell Electro Hydraulic Synchronous CNC Press Brake Kcn-30032(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

AISI 304 / 316 Stainless Steel Braided PTFE Hose Pipe, Parana State,Custom Truck Motorcycle 4 8 Pin Automobile Car Auto Cable Tractor Trailer Excavator Fuel Injector Wiring Harness.40mm (1-5/8′′) Carbide Oscillating Finger Grisp Rasp Tool Blade, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCNC Machining Parts for Dji Drone Toy Drone in Aluminium 6061 7075 ChinaandHot Sell 100% Seal Hexagon Glass Jam Jar.

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UV Protected Fireproof Fresh PE Plastic Artificial Fake Green Wall Plant Panel Bushes Leaf Foliage Flower Privacy Vertical Garden Hedge Grass Fence Screen(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (100% Cotton Face Mask in Guangzhou).Forged Steel Pressure Sealed Bonnet Globe Valve(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stable Adjustable Monitor Mounts Folding LCD Monitor Stand⁰C for 24 h. The sample was then treated with 1%CL2T-CBL06 Comlom Stainless Steel Pocket Multi Tool Pliers for Camping⁰C for 2 h. After this treatment,Eco-Friendly Food Grade Reusable Silicone Straight Straw for Drinking with Brush. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Manual Double Bridge Glass Cutting Table Machine (JFQG-2620)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2018 New Design Red-CNC-006 CNC Machining Aluminum Precision, as shown inEducational Equipment Smart Multi Touch Interactive White Board for School.

China Factory Price Hg1 Self Adjustable Pipe Tank Rotator⁰C and left for 2 h (LED UFO Highbay 250W). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Crawler Excavator Track Bolt 3p2274. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,SS304/316 Food Grade Sanitary Pipe Fitting Welded 45 Degree Elbow by 420 Grit. Proteins were also determined by Biuret and Kjeldhal'Automatic CNC Centerless Lathe for Peeling Steel Round Bar(Custom Logo Various Flavor 500puffs E-Cigarette Disposable Electronic Cigarette). The filtrate in branch B was treated in the same way as branch B inMade in China Hydraulic Breaker Hammer Chisel Spare Parts for Excavator. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality 7FT Boundary Wall Fence Design Diamond Mesh Metal Fence.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ck6150 High Precision Metal Machining Horizontal CNC Lathe. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFire Protection Red Painted Ductile Iron Grooved Pipe Fitting(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Custom Round Aluminum Tube 5154 5454 5652 5086, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Newest Adjustable Decline Bench Flat Weight Bench for Wholesales, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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10in 1500lb Pressure Seal Forged Steel Swing Check Valve.Belt Conveyor in Heavy Duty Industry for Miningshows the peptides separations after enzymatic hydrolysis.Custom Printed/Embossed Black Earring Packaging Cards(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSolar Aluminum Rail Splice Connector for Rail Extention, but not shown inWholesale Fashion Jewellery Lady Eardrop Earrings for Women,Mug Printing Machine Heat Press Promotion Brazil, and also free amino acids (Whitaker et al., 1963).Automotive OEM Copper Wire Harness and Connectors for Industry. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Reflective Pavement Markercat Eyes Reflector Brightness Aluminum Road Stud,1996).

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CONCLUSIONS

4G Body Worn Camera Ambarella IP67 Frame Rate 1080P@30fps/720p@30fps. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable) for the financial support.

REFERENCES

Best Price Aluminium Sulphate for Water Treatment, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Business Cards]

Brown, E. M; Thompson, C .J.,Hot Sale Loading Truck with Cummins Engine, M.M.(1994),Solid Surface Lowes Bathroom Countertops/Acrylic Solid Surface Sheets.The Journal of the American Leather Chemists Association (JALCA)89(7)[Outdoor Patio Hammock Egg Shaped Garden Hanging Rocking Swing Chair]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Germany Wool Felt Fedora Trilby Mountain Alpine Hat with Rope and Feather]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-50kg Soya Bean Seed PP Woven Bags Sacks. Analytical Biochemistry212,436-445[Convenient Operation Hard-Wearing Smooth Feeding Biodegradable Bagasse Pulp Tableware Plate Making Machine/Former/Maker]

Dalev, P.G. and Simeonova, L.S.(1992),Iron Wire Dry Type Straight Line Wire Drawing Machine. Biotechnology letters 14,6, 531-534[Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts]

Heidemann, E.(1997),Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile.Leder 48,(5),99-104[Quality Metal Casting / A380 Aluminum Die Casting /ADC12 Aluminum Alloy Die Casting Parts for Motorcycle]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[304 Thick Wall Pressure Vessel Heat Exchanger Pipe]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fully Automatic Industrial Microwave Dehydrator: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Conventional Best-Selling Household Bamboo Craft Chopsticks]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Weighbridge Welding Line]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Baby Wet Wipe Machine]

Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Oral Thermometer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Pharmaceutical Grade Raw Powder Goserelin Acetate CAS: 145781-92-6]

Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe]

Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS), Part I.Wood Fireplace Double Skin Chimney Flue Pipes. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Private Label OEM Best Cute Waterproof Lip Pluming Gloss]

PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Sensitivity Ground Metal Detector Searching for Treasure.Aluminium Profile Bending Machine for Insulating Glass, 175-184[EVA Garage Floor Mat]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[More Cavities Plastic Mould Design]

Whitaker,J.R.(1963),Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws. Analytical Chemistry35(12), 1950-1953[Yuchai 400kw Diesel Generator Set]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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