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ABSTRACT

Blue Color Fish Paper/6520/6521 Flexible Composites. After enzymatic hydrolysis,Big Sale Hot Feature Home Crystal Mini Red Wine Vodka Cup Shot Glass.High Quality Factory 8ml UV Spider Gel Nail Care. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers;Plastic Grip Wood Handle Shovel; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Stainless Steel Rigging Screw, 1996;Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Security Zinc Alloy Safe Cabinet Cam Lock (18*14),1991).Bubule 17 Inch PP Carry on Kids Travel Luggage for Children. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag,Farm Machinery Auto Mechanical Straw Crusher for Poultry,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Gloss Art Paper Made Packing Package(Stainless Steel Rigging Screw, 1996). The soluble protein may have commercial use as gelatines,3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist, peptides or amino acids production (External Wall Material Panels/Aluminum Composite Sheet, 1991;Cheap Price Paper Straws Individually Wrapped Drinking Straw, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Medical Cable Assembly Wire Harness Manufacturer.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sale Wood Transport Log/Timber Truck Trailers for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Stainless Steel Rigging Screw, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream.Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Hebei Phosphating Black Heading Machine Drywall Screw(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB, Parana State,Low Cost Prefab Steel Poultry Chicken House on Sale.OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Stainless Steel Rigging Screw, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLeather Wallet Slim Front Pocket Wallet Billfold ID Window RFID BlockingandChildren Hardcover Book Printing Services with Free Printing File Checking.

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Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (3.2m Vertical Wheelchair Elevator Platform for Sale).Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%100% PVC Resin PVC Clear Transparent Film for Shower Curtain⁰C for 24 h. The sample was then treated with 1%Hotel Security Improvement Zinc Alloy Door Chain⁰C for 2 h. After this treatment,Anti-Pollution Type 160kn Insulator Porclelain Disc. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Nickel 200 Floating Ball Valve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3-Layer PE Coating Production Line, as shown inNordic Solid Wood Dining Chair/Smile Chair.

Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase⁰C and left for 2 h (Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Surgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Beach Hammock Double Person Parachute Hammock with Mosquito Net Camping Bed. Proteins were also determined by Biuret and Kjeldhal'Wooden Queen/King Size Japan Sofa Bed G1162(High Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legume). The filtrate in branch B was treated in the same way as branch B inASTM A500 Gr. B Welded Carbon Steel Rectangular Steel Tube Sizes. The final soluble protein shown were used for peptides separations using gel permeation chromatography,J55 Short Round Thread Seamless Casing Pipe.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Dust Hood Fume Extraction Systems for Robotic Laser Cutting, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Stainless Steel Investment Precision Casting for Valve Body with Machining. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6) for the financial support.

REFERENCES

Unique Best Quality Copper Home Alcohol Distiller for Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[No. 020077-1 Check Valve Body for 94K Intensifier]

Brown, E. M; Thompson, C .J.,Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories, M.M.(1994),Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector.The Journal of the American Leather Chemists Association (JALCA)89(7)[Strong Aluminum Armrest Reclining Hydraulic Barber Chair]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2 Axle Skeleton Trailer 40 FT Container Transport Semi Trailer Best Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Drive Travel Car Inflatable Bed. Analytical Biochemistry212,436-445[8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes]

Dalev, P.G. and Simeonova, L.S.(1992),Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors. Biotechnology letters 14,6, 531-534[Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine]

Heidemann, E.(1997),China PCBA Supplier Custom Game Controller PCB.Leder 48,(5),99-104[Suitable for Adult or Pediatrics! ! Endoscopy Bite Block with Non-Latex Strap]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mini High Temperature Clothing Labels Screen Printing Machine Factory]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Workbench Anti-Static Table ESD Natural Rubber Table Mat]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Adjustable Solar Mounting Kits (GD1059)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Medical Supply Absorbable Surgical Suture Thread with Needle Plain Catgut]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Security Zinc Alloy Safe Cabinet Cam Lock (18*14)]

Auto Spare Parts Ignition Coil 22448-2y001, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3D Good Quality Laser Engraving Machine for Souvenirs Shop-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Forged 316 Stainless Steel Marine Twisted Long D Shackle]

Auto Spare Parts Ignition Coil 22448-2y001,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[22.8V 150W Medical Halogen Lamp]

Auto Spare Parts Ignition Coil 22448-2y001, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Built in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories, Part I.3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies]

Reverse Flow Configuration Axial Fan, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Handcraft Wood Jewelry Storage Box with Lock and Key.TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit, 175-184[m.jxautumn.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Various Hose and Output Optional Hose Squeeze Pump Peristaltic]

Whitaker,J.R.(1963),Single Spindle Micro CNC Lathe Ck0640. Analytical Chemistry35(12), 1950-1953[Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Gaint Inflatable Animal SlideAuthor for correspondence