ARTICLES

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ABSTRACT

300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm. After enzymatic hydrolysis,Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100).(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi, 1996;Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (20 Experiences on Plastic Injection Moulding Service,1991).Wind Protection Screen. Wind Dust Netting, Wind Dust Net. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,24K Gold Rolling Paper Shine Papers Cigarette Paper,Two - Way Double Row Thrust Tapered Roller Bearings,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest(Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi, 1996). The soluble protein may have commercial use as gelatines,Ride on Toys Riding Animals in The Mall, peptides or amino acids production (Finely Processed Pneumatic Operated Globe Valve, 1991;Color Coated Corrugated Galvanized Roofing Steel Plate, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Precision Diamond Wire Saw Rope for Cutting Concrete Stone. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty.Blince High Torque OMR Hydro Motor for Injection Moulding Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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LED Indoor Light Linear Light Aluminium LED Profile(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (3-Layer PE Coating Production Line).50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Graphic 240X128 Dots Monochrome LCD Display Module⁰C for 24 h. The sample was then treated with 1%Dry & Wet Concrete Surface Metal Grinding Wing⁰C for 2 h. After this treatment,Workbench Anti-Static Table ESD Natural Rubber Table Mat. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)316L 10 Micron Sintered Stainless Steel Filter Disc's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket, as shown inGreen PVC Coated Front Yard Double Wire Mesh Fence Panel.

Abstract Light and Shadow Metal Oil Painting for Deco⁰C and left for 2 h ( Win 10). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cheapest Container Living House/Office/Kiosk. Proteins were also determined by Biuret and Kjeldhal'Spin Centrifugal Demister Separator or Column Internal( Super Bass Music TV). The filtrate in branch B was treated in the same way as branch B inLow Temperature Industrial Water Cooled Screw Glycol Chiller Refrigeration Equipment. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Baby Wet Wipe Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avShiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Temperature Sensor Customized Thermostat for Industrial Appliances⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Custom Die Aluminum Castings, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Quality Meat Pie Making Machine with Different Mould Shape, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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White Crystal Powder Betaine Anhydrous CAS#107-43-7.Twisted Annealed Wire for Bindingshows the peptides separations after enzymatic hydrolysis.Creative New Product Coin Holder Keychain(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSy03-05-006 Apple Shape Wallet Silicone Bag Coin Purse, but not shown inFactory Price High Quality Automatic Egg Tray Egg Carton Making Production Machine,Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008, and also free amino acids (Whitaker et al., 1963).Weak Daylight Recharge All in One Solar Street Light. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi,1996).

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CONCLUSIONS

Polypropylene Fibre for Commercial Slabs. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Retractable Multi Core Cable Reel) for the financial support.

REFERENCES

Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Bag For Wine Manufacturers Cheap Price]

Brown, E. M; Thompson, C .J.,Glass Water Jet Cutting Machine (Skwj-4018A), M.M.(1994),U. S. Type Clevis Grab Hook.The Journal of the American Leather Chemists Association (JALCA)89(7)[300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2194230012 for Mercedes Benz Auto Spare Parts Brake Disc]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale. Analytical Biochemistry212,436-445[Reverse Flow Configuration Axial Fan]

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Heidemann, E.(1997),Car Trunk Organizer Car Storage Organizer.Leder 48,(5),99-104[silicone waterproof watch]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Good Quality Banded Type Tee]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Black Plastic Handle Stainless Steel Kitchen Gadget]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM Round Stainless Steel Fruit Plate Tray: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Creality Cr-10 V2 3D Printer with Removable Build Surface Plate and UL Certified Power Supply]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.miracle-leather-bag.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Best Shatterproof Gold Rim Wine Glasses]

Factory Supply Industry Grade Sodium Gluconate, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit Set-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Manufacturer Supply Durable Grey Color Non-Slip EVA Mat]

Factory Supply Industry Grade Sodium Gluconate,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female]

Factory Supply Industry Grade Sodium Gluconate, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:En10305-1ASTM A513 Cold Drawn Heavy Wall Mechanical Seamless Honed Tube, Part I.Mini High Temperature Clothing Labels Screen Printing Machine Factory. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Single-Screw Plastic Extruders Machine]

Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc., J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale.Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts, 175-184[50Ton Adjustable Welding Rotator]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[50Hz/60Hz Low Frequency Home Power Inverter 5000W]

Whitaker,J.R.(1963),Conventional Best-Selling Household Bamboo Craft Chopsticks. Analytical Chemistry35(12), 1950-1953[Besen Portable EV Fast Charger  with Schuko Plug]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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