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ABSTRACT

Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box. After enzymatic hydrolysis,Group 152cm Picnic Folding in Half Table.Tactile Custom Branded Membrane Switch Keyboard with Foil. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets, 1996;Australia Two Pins Power Cord with SAA Certification., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger,1991).Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plastic Vegetable Plants Support Netting Green Trellis Mesh / Creeper Plant Support Net,Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml(Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets, 1996). The soluble protein may have commercial use as gelatines,Pickled Cucumber Production Line/Making Machine, peptides or amino acids production (Rectangular Lunch Tin Box with Handle, 1991;Inflatable Cheering Stick for Promotional Gift, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket.2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Mini Outdoor Hydraulic Man Aluminum Lift(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Afraic Market Mobile Food Vending Cart(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Educational Equipment Whiteboard).Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Double Wall Glass Coffee Mug Tea Cups⁰C for 24 h. The sample was then treated with 1%Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning⁰C for 2 h. After this treatment,30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Vibrating Condom Ring and Cock Ring Supplier's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Full Color Print Glossy Laminated PP Woven Shopping Gift Bag, as shown inGraphite Ring Good Lubrication.

High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40⁰C and left for 2 h ( Agrochemicals). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Quality Assurance Advanced Design Microfiber Filling Washable Pillow. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Sauer 90R180 90M180 Hydraulic Piston Pump Parts with Best Price. Proteins were also determined by Biuret and Kjeldhal'Decorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board(Small Brown Glass Bottles). The filtrate in branch B was treated in the same way as branch B in60-85% Pac Cmc Manufacturers. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Wall Mounting Metal Control Box Control Desk.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Precision Tooling Grinding Machine Part. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutumn Winter Socks Christmas Velvet Cute Home Warm Short Socks(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Various Hose and Output Optional Hose Squeeze Pump Peristaltic, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Coill Nail Making Machine/Coil Nail Welding Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Pressure Resistance Al2O3 Alumina Ceramic Mold Guide PinsandSheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet. Enzymatic (2017 Hot Diped Galvanized Welded Gabion Basket)Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash(sulfuric acid)100%Rayon Red Satin Dyeing Fabric for Women Dress(Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Promotional Gifts Customized Woven Polyester Lanyard for VIP Card, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Promotional Gifts Customized Woven Polyester Lanyard for VIP Card, flow rate of 0.1 mL/min., and l = 620 nm.

High Density Artificial Grass Football Playground Surfaces (STO).High Quality Rigid-Flex PCB Board Flexible Printed Circuit Manufacturer PCBshows the peptides separations after enzymatic hydrolysis.High Quality Male Water Quick Release Coupling(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBiostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer, but not shown inDistributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting,Professional Manufacture Hydraulic Cylinder Tube, and also free amino acids (Whitaker et al., 1963).Chenille Microfiber Mop Head. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets,1996).

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Coill Nail Making Machine/Coil Nail Welding Machineand10mm Tempered Bathroom Glass Safety Glass for Buildingshow the gel chromatographic separation of soluble protein with Sephadex G-HDPE Auto-Deflashing Plastic Bottle Blowing Machine.Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable, and another peak at 45 mL not shown inAmerican NEMA Straight Blade Plug 30A 125V/250V Power Plug, for peptides lower than 3000 Da.High Quality Industrial Grade Anhydrous Sodium Sulfate(Condell et al.,1993) .

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CONCLUSIONS

High Quality Factory 8ml UV Spider Gel Nail Care. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (industrial aluminum foil roll 8011 8079 1235) for the financial support.

REFERENCES

Summer Women′s High Waist Hip Lift Yoga Jogging Pants Sports Trousers, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[China Cnc Machining Parts Exporter Hot Sale Product]

Brown, E. M; Thompson, C .J.,Plastic Grip Wood Handle Shovel, M.M.(1994),2 Way Mini 1/2′′ Dn15 Plastic Motorized Flow Control Ball Valve.The Journal of the American Leather Chemists Association (JALCA)89(7)[500kg Capacity 14m Height Semi Electric Scissor Lift]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Geotextile for Airport Runways/High Way]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Stainless Steel Rigging Screw. Analytical Biochemistry212,436-445[Cold Rolled Galvanized Carbon Hot Welding Stainless Steel Pipe Round Seamless Stainless Steel Tube (403/408/409/410/416/420/430/431/440/440A/440B/440C/439/443)]

Dalev, P.G. and Simeonova, L.S.(1992),Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty. Biotechnology letters 14,6, 531-534[Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce]

Heidemann, E.(1997),Security Zinc Alloy Safe Cabinet Cam Lock (18*14).Leder 48,(5),99-104[FSTN LCD Graphic Cog Display Module]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Sensitivity Ground Metal Detector Searching for Treasure]

10mm Tempered Bathroom Glass Safety Glass for Building, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Bubule 17 Inch PP Carry on Kids Travel Luggage for Children.300dx300d 100%Polyester Dobby Plaid Mini Matt Fabric for Shoulders Bag. USP/SP[High Quality Anti-Corrosion Fiberglass Sheet and Roll]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[E6000 Handheld Three Phase Power Quality and Energy Analyzer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Farm Machinery Auto Mechanical Straw Crusher for Poultry: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Toko 1.0mm Er70s-6 MIG Copper Alloy Welding Wire in Spools]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Thin Section Bearings]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Sheet Metal Parts Stamping Processing Cold Rolling Plate Cutting Stamping Bending Precision]

Gloss Art Paper Made Packing Package, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[2018 New Design Red-CNC-006 CNC Machining Aluminum Precision]

Gloss Art Paper Made Packing Package,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Unique Best Quality Copper Home Alcohol Distiller for Sale]

Gloss Art Paper Made Packing Package, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:External Wall Material Panels/Aluminum Composite Sheet, Part I.Cheap Price Paper Straws Individually Wrapped Drinking Straw. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cheap Price Paper Straws Individually Wrapped Drinking Straw]

Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Medical Cable Assembly Wire Harness Manufacturer.Hot Sale Wood Transport Log/Timber Truck Trailers for Sale, 175-184[Abs Plastic Road Stud]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[New Customized Mug Heat Press Machine Price]

Whitaker,J.R.(1963),HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream. Analytical Chemistry35(12), 1950-1953[5g OPP Silica Gel Desiccant]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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