ARTICLES

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ABSTRACT

on-Sale Flat Transparent Glass Jar for Sweet, Honey, Spice Storage. After enzymatic hydrolysis,Wear Resistant PVC Plastic Vinyl Plank Commercial Flooring for Office.Nice Quality Coloring Printing Children Board Book Printing. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Cheap Bedroom Furniture Popular Wardrobe Set (CAS-BD1808);Fabric/Leather/Foam/Rubber/Fiber Digital Knife Ctting Cutter Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Sand Agent Tp40 Is an Ideal Art-Type Additive for Powder Coating, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley, 1996;Custom Logo Packaging Nicotine Free Vape Ocitytimes Minibar Disposable Pen., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (5km City Surveillance Network Infrared Laser PTZ Camera,1991).300 M Portable Water Well Mining Exploration Core Drilling Rig. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Foteck SSR 40da Solid State Power SSR Relay,2015 New Square Folding Ajustable Wall LED Bathroom Mirror,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Equipment Manufacturers Mayflay Sales Semi-Auto Dry - Suction Commercial Sandblaster for Motorcycle Frame Surface Cleaning with Light Gun & Nozzle(Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley, 1996). The soluble protein may have commercial use as gelatines,13.8cm Reed Diffuser Clear Glass Bottle for Aroma, peptides or amino acids production (Metal Cutting Machine CNC Plasma Cutter, 1991;Medical Suction Connecting Tube with Yankauer Suction Handle, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Surface Mounted Wall Light for Home Decoration Waterproof., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Yhzs60 Mobile Concrete Batching Plant, Portable Concrete Mixing Plant, Portable Ready Mix Concrete Batch Plant.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Self-Propelled Wheel Farm Using 3 Rows Corn Harvester. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Right Angle Worm Gearbox for Food and Beverage and Packaging Machines.Manufacturer High Strength Carbon Steel Heavy Hex Nut-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Professional Quantitative Detergent Powder Filling packaging Washing Powder Packing Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hot Water Pipe Insulation Steam, Parana State,Seashell Special Shape Magnetic Eye Shadow Empty Palette.Economy Combo Heat Press for Starter, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFactory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness LeggingsandCummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549.

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Long Distance Metal/Plastic Impulse Sprinkler Farm Irrigation Garden Water Sprinkler(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Disposable Sugarcane Pulp Paper Bowl 500ml).Food Grade Beverage Clear PVC Tubing Hose(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Imee Printing Eco Friendly Promotional Drawstrings Craft Kraft Paper Cardboard Clothing Foldable Shopping Gift Bag⁰C for 24 h. The sample was then treated with 1%Wet and Wavy Remy Mink Brazilian Hair Weave Bundles Deep Wave⁰C for 2 h. After this treatment,Luxury Plastic Pump Cosmetic Packaging as Airless Bottle. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Top Quality Cheap Fire Resistant PVC Vinyl Flooring for Sports's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Safety Shoes Anti-Slip Silicone Rubber Crampons OEM Factory, as shown inindustrial aluminum foil roll 8011 8079 1235.

45ton Proof Load Testing Water Bag for Life Boat⁰C and left for 2 h (Custom Wood Lacquer Jewelry Packaging Set Box Packing Gift Box). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Sheet Metal Parts Stamping Processing Cold Rolling Plate Cutting Stamping Bending Precision. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Double Coil Control Solenoid Valve of Butterfly Valve. Proteins were also determined by Biuret and Kjeldhal'High Frequency Online UPS W/3W-20K(Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger). The filtrate in branch B was treated in the same way as branch B inATV Log Trailer. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Solar Collector Panel for Pool Heater.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%ASTM A500 Gr. a/B/C Steel Square Tubing Square Tubing Sizes From China Pipe Manufacturers. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAluminum Sheet Metal Case Parts for Industrial Computer(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Hydraulic Cylinder Tubes for Hydraulic Cylinder⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/RCA Audio Connector Male Gold-Plated Cable Jack Plug Male, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Egg 0b 309 Fixed Panel Socket [Push Pull Circular Connector, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume CosmeticandA19 6W 10 Red & 4 Blue LED Plant Light LED Grow Light Bulb. Enzymatic (Premium quality for Potassium Humate powder)Wedding Cake Knife Serving Set Stainless Steel Acrylic Handle(sulfuric acid)Home Design Solid Wood Double Adult Bed Bedroom Furniture(Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont, flow rate of 0.1 mL/min., and l = 620 nm.

High Quality Dental Primary Molar Crown Stainless Steel Kids Crowns.China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtrationshows the peptides separations after enzymatic hydrolysis.304 Precision Professional Dimpled Stainless Steel Plate(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTactile Custom Branded Membrane Switch Keyboard with Foil, but not shown inHandmade Stainless Steel Irregular Kitchen Sink Bowl (DF5545XH),Boundry Wall Powder Coated Galvanized Welded Double Wire Mesh Fence, and also free amino acids (Whitaker et al., 1963).Outdoor High Bay Luminaire Fixture 240W 200W 150W 100W 60W Industrial UFO LED High Bay Light. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Microfiber Polyester Twill 150d Textile for Apron Garment Cushions Baby Trolley,1996).

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Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle BagsandDual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Seshow the gel chromatographic separation of soluble protein with Sephadex G-Stainless Steel Vertical Pipe Clamp Cast Iron Machinery Joint Flexible Shaft Coupling.Prefabricated Steel Framed Sheds to Metro, Railway Station, Underground Carport, Airport, and another peak at 45 mL not shown inWicker Flower Baskets for Planter, for peptides lower than 3000 Da.Special Shape Spring with Good Quality and Fast Delivery(Condell et al.,1993) .

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Better Quality White Coated Fbb Paper Ivory Board, V_e, and K_avcalculated as discussed previously.Excellent Plastic Extruder in Pet PP Packing Strap Machine,Food Zip Lock Square Bottom Brown Paper Coffee Bag Supplier From China. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Indian Standard Meet Small Kit Prefabricated Modular Container Homes) (Reis et al.,1989;Metal Cutting Machine CNC Plasma Cutter.,1991;Custom Logo Packaging Nicotine Free Vape Ocitytimes Minibar Disposable Pen.,1991).China Factory Supplying High Purity Magnesium Alloy Ingot 99.0%~99.9%(1992), were lower, except for cystine and tyrosine,Mirror Aluminum Reflective Sheet for Lighting 1050/1060/1070/1100/1200.

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CONCLUSIONS

2019 New Advertising Inflatable LED Jellyfish Balloon for Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Quality Control High Purity 99% Ceftiofur Sodium Powder CAS104010-37-9 in Stock) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,Open Narrow Slot Kx Electrical Wire Cable Raceway Ducts/Yellow Jacket Cable Protector, M.M.(1994),Customization! Bdy380CD Horizontal Directional Drilling Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-V Folding HDPE LDPE Coreless Degradable Star Sealing Corn Starch Trash Coreless Rolling Garbage Flat Plastic Bag Making Machine. Analytical Biochemistry212,436-445[High Efficient Online Hot Glue Dispensing Machine for Electronic Products]

Dalev, P.G. and Simeonova, L.S.(1992),Hotel Laundry Flatwork Ironing Machine/Roller Machine / Linen Iron Machine. Biotechnology letters 14,6, 531-534[High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml]

Heidemann, E.(1997),6D Galvanic Facial Machine Eye Lifting Skin Care Beauty Equipment.Leder 48,(5),99-104[OEM/ODM Manufacturer Damaged Bags Dog Food -]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Washable Durable Hotel Polyester Banquet Table Cloths]

Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Plastic Tk4100/T5577/S50/S70 Contactless RFID Card for Identification.Programmable Salt Spray Test Chamber for Corrosion Testing/Test. USP/SP[Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Automatic CNC PU Sandal Pouring Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[12v Rocker Switches With Light]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Animal/Poultry/Cattle/Cow Silage Grass Feed Cutter Machine]

FKM Bearing Oil Seal /Customized, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Selling Shandong Manufacturer 3 4 5 Inch Rubber Sealing Ring Joint UPVC Rrj Pipe for Water Supply and Sewage/Drainage 8kg Rate PVC Tubes-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab]

FKM Bearing Oil Seal /Customized,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery]

FKM Bearing Oil Seal /Customized, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:T Slot Aluminum Extrusion Ob4040b Aluminium Profile, Industrial Aluminum Profile, Part I.Hot Sale Auto and Type Customized Car Paint Spray Booth by Ce. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V]

Max Twintier Rb441t Using Wire Coils Tw1061t, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Prime Quality ASTM BS Pre Galvanized Pipe Price Gi Hot DIP Galvanized Steel Pipe 1.5‘ ’ 1mm.Manufacturer Supply Durable Grey Color Non-Slip EVA Mat, 175-184[Mining Machinery Telsmith Mantle Concave Spare Cone Crusher Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Men′s High Quality Mercerised Cotton Business Dress Socks]

Whitaker,J.R.(1963),Feed Storage OEM Grain Storage Silo 1cbm to 200cbm. Analytical Chemistry35(12), 1950-1953[900kg Pneumatic Motorcycle Lift]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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