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ABSTRACT

Full Automatic AAC Concrete Lightweight Block Making Machine. After enzymatic hydrolysis,Stainless Steel CF8/CF8m Street Elbow Pipe Fittings.Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Low Volume Production Aluminum CNC Machining Auto Parts, 1996;Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank,1991).Carbon Steel Round/Square Pipe Tube Production Line. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Green PVC Coated Front Yard Double Wire Mesh Fence Panel,Cable Length 11 Gun Safety Padlock,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Canvas School College Backpack/Bookbags for Girls/Students/Women(High Quality Low Volume Production Aluminum CNC Machining Auto Parts, 1996). The soluble protein may have commercial use as gelatines,Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles, peptides or amino acids production (Women′s Seamless Strapless Bra, 1991;Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Low Volume Production Aluminum CNC Machining Auto Parts, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw.Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (New Fashion Diaper Bags Mummy Bag).Ride on Toys Riding Animals in The Mall(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Finely Processed Pneumatic Operated Globe Valve⁰C for 24 h. The sample was then treated with 1%Color Coated Corrugated Galvanized Roofing Steel Plate⁰C for 2 h. After this treatment,Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Precision Diamond Wire Saw Rope for Cutting Concrete Stone, as shown in12V 24V 36V 72V 84V Customized Lithium Ion LiFePO4 Battery Charger for E-Bike.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Retractable Multi Core Cable Reel. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Xcm 120 Asphalt Mixing Plant for Sale(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/LED Indoor Light Linear Light Aluminium LED Profile, pH 3.25., mobile phase B: buffer solution of 0.01 mol/50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Abstract Light and Shadow Metal Oil Painting for Deco.Food Grade Ivory Paper Cupcake Boxesshows the peptides separations after enzymatic hydrolysis.Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003)(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume, but not shown inOEM Round Stainless Steel Fruit Plate Tray,Cheapest Container Living House/Office/Kiosk, and also free amino acids (Whitaker et al., 1963).Spin Centrifugal Demister Separator or Column Internal. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Quality Low Volume Production Aluminum CNC Machining Auto Parts,1996).

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CONCLUSIONS

China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (20 Experiences on Plastic Injection Moulding Service) for the financial support.

REFERENCES

Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Extruder for PP/PS/ABS Plastic Sheet]

Brown, E. M; Thompson, C .J.,Wholesale Universal Garden Smart Flower Pot, M.M.(1994),Reflective Tape for Vehicle Reflective Markings (tape-02).The Journal of the American Leather Chemists Association (JALCA)89(7)[5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Car Trunk Organizer Car Storage Organizer]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-White Crystal Powder Betaine Anhydrous CAS#107-43-7. Analytical Biochemistry212,436-445[Manufacturer CNC High Precision Machining Metal Parts Customized for Auto]

Dalev, P.G. and Simeonova, L.S.(1992),Creative New Product Coin Holder Keychain. Biotechnology letters 14,6, 531-534[Small Capacity Automatic Cup Filling Machine]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China High Efficiency Automatic Glue Dispensing Machine with Factory Price]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Epon ONU with 4ge 2VoIP Pots 2.4G 5g Twon Antenna WiFi Gepon Ont]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Rigid PVC/ Rigid Floor /Rigid Flooring /Spc Flooring]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Combustion Chamber]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Quality Aluminum Carpet Topping Stage Platform]

3mm Indoor Wall Cladding Decoration Aluminum Composite Material, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Professtional Suppliers Yutong Golden Dragon Bus Spare Parts]

3mm Indoor Wall Cladding Decoration Aluminum Composite Material,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Economy Combo Heat Press for Starter]

3mm Indoor Wall Cladding Decoration Aluminum Composite Material, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automatic CNC PU Sandal Pouring Machine, Part I.Glass Disc Suspension Insulators. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Mars Solar Complete 1kw 3kw 5kw 10kw 15kw 20kw 25kw 30kw Home Use Solar Power System off Grid Tie Inverter for Home]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack]

Whitaker,J.R.(1963),Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module. Analytical Chemistry35(12), 1950-1953[Original Sinotruk HOWO Truck Parts - Exhaust Pipe Wg9725542042]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

304 Precision Professional Dimpled Stainless Steel PlateAuthor for correspondence