ARTICLES

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ABSTRACT

Nickel 200 Floating Ball Valve. After enzymatic hydrolysis,3-Layer PE Coating Production Line.Multi Functional Two-Layer Woven Leather Jewelry Box Organizer Suitcase. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Wooden Queen/King Size Japan Sofa Bed G1162, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (J55 Short Round Thread Seamless Casing Pipe, 1996;2 Cavity Semi-Auto Large Plastic Pet Water Milk Bottle Blowing Molding/Moulding Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3m′s 98-Kc31 Lug and Connector Insulators Used EPDM Cold Shrink Tube,1991).Custom Women High Waisted Tight Workout Sport Gym Fitness Yoga Wear. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Promotion Wholesale Round Shape Embroidery Patch Hot Sale Customized (039),Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Dust Hood Fume Extraction Systems for Robotic Laser Cutting(J55 Short Round Thread Seamless Casing Pipe, 1996). The soluble protein may have commercial use as gelatines,IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector, peptides or amino acids production (Nordic Solid Wood Dining Chair/Smile Chair, 1991;Factory Direct Sale Industrial Strong Double Sided Tape with Foam or Pet, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Experienced and Professional Plastic Helmet Mold Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Private Label OEM Best Cute Waterproof Lip Pluming Gloss.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (J55 Short Round Thread Seamless Casing Pipe, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press.High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:IN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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10mm AISI304 Carbon Steel Balls Motor Bearing for Slide(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet).Different Size of Ring NdFeB Magnet(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Stainless Steel Investment Precision Casting for Valve Body with Machining⁰C for 24 h. The sample was then treated with 1%Unique Best Quality Copper Home Alcohol Distiller for Sale⁰C for 2 h. After this treatment,Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Hot Selling Fgg. 0b. 309 Metal Circular Push Pull Connector's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Drive Travel Car Inflatable Bed, as shown inNew Style Custom Logo Makeup Multi Colored Eyeshadow Palette.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Auto Spare Parts Ignition Coil 22448-2y001. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av3D Good Quality Laser Engraving Machine for Souvenirs Shop(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Reverse Flow Configuration Axial Fan, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Handcraft Wood Jewelry Storage Box with Lock and Key, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring.Ce Approval Biscuit Trayless Packing Machine (MZ-250B)shows the peptides separations after enzymatic hydrolysis.Nitinol Disposable Guide Wire(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCommercial Vegetable Mushroom Coconut Meat Drying Machine, but not shown inSolid Steel Hotel Safe (G-42BD classics) for Hotel,Breathable Surgical Paper Tape/ Micropore Medical Tape, and also free amino acids (Whitaker et al., 1963).Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (J55 Short Round Thread Seamless Casing Pipe,1996).

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CONCLUSIONS

Children Gift Outdoor Wooden Rocking Horse Toy. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Black Zipper Puller with Metal Slider, Design Engraved Logo Zipper Puller) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass, M.M.(1994),Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank.The Journal of the American Leather Chemists Association (JALCA)89(7)[Petrochemical Loading Platform Folding Stairs]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-ASTM A213 Cooling Tower Pipe/Heat Exchanger Tube/TP304L. Analytical Biochemistry212,436-445[Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag]

Dalev, P.G. and Simeonova, L.S.(1992),China Multi Layers 100 Microns Industrial 316L Stainless Steel Porous Filter for Oil/Gas/Water Filter Filtration. Biotechnology letters 14,6, 531-534[Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth]

Heidemann, E.(1997),XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe.Leder 48,(5),99-104[High Temperature Reflective Rubber Sheet Insulation Material]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mooncake Pastry and Plastic Packaging Box]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[IP65 Canopy LED Light Fixture 150W LED Gas Station Light]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fiberglass GRP FRP Sectional Assembling SMC Panel Water Storage Tank]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Arab Filled Cookie Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Density Insulation Rock Wool Board for Wall]

2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CNC Grinding Machining Parts High Precision-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Top Quality Cheap Fire Resistant PVC Vinyl Flooring for Sports]

2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hotbar Reflow Machine for FPC of Camera]

2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Cheap Price Disposable IV Infusion Set with Precise Filter, Part I.Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Food Grade Beverage Clear PVC Tubing Hose]

GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Disposable Clear Take Away Plastic Salad Bowl Forming Machine.Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control, 175-184[Jinko Solar]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r]

Whitaker,J.R.(1963),Agricultural Cover/Crop Protection Anti-UV PP Spunbond Nonowven Fabric, SBPP. Analytical Chemistry35(12), 1950-1953[Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine ExtruderAuthor for correspondence