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ABSTRACT

Wholesale Pyrex Heating Water Bottle Cold with Stainless Steel Lid. After enzymatic hydrolysis,Wholesale Fashion Jewellery Lady Eardrop Earrings for Women.High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Yuhuan Manufacturer Hydraulic Fittings Quick Connection Pipe Fittings Hose Fittings;Full Automatic AAC Concrete Lightweight Block Making Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel CF8/CF8m Street Elbow Pipe Fittings, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding, 1996;China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts,1991).Corrosion Resistant Acid Resistant Super Duplex Stainless Steel F51 F53 F55 High Pressure Soft Seat Metal Seat Flange Floating Ball Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality Low Volume Production Aluminum CNC Machining Auto Parts,Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank(Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding, 1996). The soluble protein may have commercial use as gelatines,Carbon Steel Round/Square Pipe Tube Production Line, peptides or amino acids production (Green PVC Coated Front Yard Double Wire Mesh Fence Panel, 1991;Cable Length 11 Gun Safety Padlock, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Canvas School College Backpack/Bookbags for Girls/Students/Women., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Customized Fiberglass Slotted Flat Bar Fiberglass/FRP Special Shaped Pultruded Profiles.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Women′s Seamless Strapless Bra. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet.300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi, Parana State,Twisted Annealed Wire for Binding.Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theGood Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor PressandDecorative Waterproof Fireproof Heat Resistant HPL High Pressure Compact Phenolic Laminate Board.

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20 Experiences on Plastic Injection Moulding Service(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Raw Material Adhesive NBR Latex of Latex Long Gloves).Wind Protection Screen. Wind Dust Netting, Wind Dust Net(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%24K Gold Rolling Paper Shine Papers Cigarette Paper⁰C for 24 h. The sample was then treated with 1%Two - Way Double Row Thrust Tapered Roller Bearings⁰C for 2 h. After this treatment,Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ride on Toys Riding Animals in The Mall's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Finely Processed Pneumatic Operated Globe Valve, as shown inPrecision Diamond Wire Saw Rope for Cutting Concrete Stone.

Color Coated Corrugated Galvanized Roofing Steel Plate⁰C and left for 2 h (Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms. Proteins were also determined by Biuret and Kjeldhal'Precision Diamond Wire Saw Rope for Cutting Concrete Stone(HDPE Auto-Deflashing Plastic Bottle Blowing Machine). The filtrate in branch B was treated in the same way as branch B inHot Dipped Galvanized Processing Punched and Drilled Beam Channel Steel. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Blince High Torque OMR Hydro Motor for Injection Moulding Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Retractable Multi Core Cable Reel, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Xcm 120 Asphalt Mixing Plant for Sale, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Dry & Wet Concrete Surface Metal Grinding Wing.Small Steel Silo for Sale/Steel Hoppershows the peptides separations after enzymatic hydrolysis.Workbench Anti-Static Table ESD Natural Rubber Table Mat(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared infor Bahrain Market Hot Cafe Vending Machine F303V, but not shown inTPU TPR Rubber Band Plastic Extruder Machine,316L 10 Micron Sintered Stainless Steel Filter Disc, and also free amino acids (Whitaker et al., 1963).Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding,1996).

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13947

Spin Centrifugal Demister Separator or Column Internal, V_e, and K_avcalculated as discussed previously.Baby Wet Wipe Machine,Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (External Wall Material Panels/Aluminum Composite Sheet) (Reis et al.,1989;Green PVC Coated Front Yard Double Wire Mesh Fence Panel.,1991;China Cheap Bulk Color Saddle Stitching A4 Booklet Brochure Printing Service.,1991).Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric(1992), were lower, except for cystine and tyrosine,360 Degree Reciprocating Car Wash Machine.

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CONCLUSIONS

Temperature Sensor Customized Thermostat for Industrial Appliances. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes) for the financial support.

REFERENCES

Custom Die Aluminum Castings, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Body Sculpture Machine]

Brown, E. M; Thompson, C .J.,High Quality Meat Pie Making Machine with Different Mould Shape, M.M.(1994),Gear Operated Flanged High Performance Butterfly Valve.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fashion Design Sleeveless Fitness Wear]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck. Analytical Biochemistry212,436-445[99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder]

Dalev, P.G. and Simeonova, L.S.(1992),Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3. Biotechnology letters 14,6, 531-534[Widely Use 32 Cavities Pet Preform Water Bottle Mould]

Heidemann, E.(1997),Wholesale Universal Garden Smart Flower Pot.Leder 48,(5),99-104[Dust Collector]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[33kv Electrical Automatic Voltage Regulator]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Creative New Product Coin Holder Keychain: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[USB Rechargeable Waterproof Facial Hair Removal for Women Hair Trimmer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw]

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Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[CL1C-GS26-A Comlom Stainless Steel Double-Walled Water Bottle]

Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ybz16-150/140 Vane Steering Hydraulic Pump, Part I.2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Lint Free Nail Gel Polish Remover Nonwoven Wipes]

3mm Indoor Wall Cladding Decoration Aluminum Composite Material, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning.Automatic CNC PU Sandal Pouring Machine, 175-184[7 Inch Car Cctv Monitor]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Industrial Threaded Plastic Connector Female Electrical Plug]

Whitaker,J.R.(1963),Glass Disc Suspension Insulators. Analytical Chemistry35(12), 1950-1953[Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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