ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Iron Wire Dry Type Straight Line Wire Drawing Machine. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile

ABSTRACT

Barricade Tape. After enzymatic hydrolysis,High Brightness White Color IP20 SMD5050 Chip 30LEDs 7.2W DC24V LED Strip.Fully Automatic Industrial Microwave Dehydrator. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Air Compressor Storage Tank Air Compressor Receiver Air Tank (300L);Oral Thermometer; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wood Fireplace Double Skin Chimney Flue Pipes, 1996;PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Sensitivity Ground Metal Detector Searching for Treasure,1991).Aluminium Profile Bending Machine for Insulating Glass. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws,304 Thick Wall Pressure Vessel Heat Exchanger Pipe,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Betaine Anhydrous with Best Price107-43-7(Wood Fireplace Double Skin Chimney Flue Pipes, 1996). The soluble protein may have commercial use as gelatines,FM1108 Chip Bulk Blank Magnetic Stripe RFID PVC Cards, peptides or amino acids production (Extra Heavy Wall Thickness Seamless Tubes, 1991;Shrink Sleeves Labels for Packaging Labeling, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;IP65 Canopy LED Light Fixture 150W LED Gas Station Light., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Plastic Mould Dongguan Molding Factory.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wood Fireplace Double Skin Chimney Flue Pipes, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Color Coated Aluminum Coil/PPGI/PPGL/Gi/Gl and Roofing Sheets 3003 3004.Factory Industrial Fast Digital Glass Ceramic Tile Plastic Aluminum Flatbed UV Printer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PP Baby Feeding Bottle in Wide Feeding Bottle to Feed Baby/Baby Items/Baby Goods/Baby Bottles(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Inclined Waste Water Treatment Lamella Clarifier(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Canvas School College Backpack/Bookbags for Girls/Students/Women).33kv 100kVA Dry-Type Distribution Transformer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Fruit/ Vegetable Double Spray High Pressure Cleaner for Sale⁰C for 24 h. The sample was then treated with 1%Fine 36 Hydraulic Breaker Seal Kit for Fine 36 Hydraulic Hammer Seal Kit Fine 36 Breaker Seal Kit⁰C for 2 h. After this treatment,5mm Spc Vinyl Planks Click Lock Floor PVC Plastic Laminate Flooring. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Suoer 12V 220V 3000W Pure Sine Wave Inverter (FPC-D3000A)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Rimmed 1 2 5 10 15 20 25 Micron Circular Stainless Steel Filter Disc , as shown inGood Quality Custom Car Power Window Cable for Ford Car.

China Manufacturer Export Copper Foot Tap/Valve (HFV-001)⁰C and left for 2 h (5 in 1 Hydra Facial Peeling Micro Dermabrasion Skin Care Skin Whitening Multifunction Beauty Equipment). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Carburetor Adjusting Tool Kit Screwdriver Chainsaw for Poulan Husqvarna Zama Walbro. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,PVC Corrugated or Smooth Surface Helix Suction Water Hose. Proteins were also determined by Biuret and Kjeldhal'Cleaning Pipeline Cleaning Rubber Sponge Ball for Concrete Pump Spare Parts(High Quality Dyed Colored Sand). The filtrate in branch B was treated in the same way as branch B in165061sk-Mens Bamboo Cotton Causal Dress Pattern Socks. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Ivd Component PP Barbed Threaded Connector.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Workshop Tools 7.5 Ton Electric Winch with Dual Speed. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBest High Energy 1500mj 1064nm 532nm Painless Permanent Age Spot Freckle Tattoo Removal Skin Laser Therapy Equipment(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.500ml Stainless Steel Thermos Glass Sport Flask Vacuum Kettle Double Wall Water Bottle⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/RFID Blocking Leather Slim Zipper Credit Card Holder Wallet, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2017 New Design Vinyl 3D Stone Wallpaper Factory in China, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Quality D400 FRP Composite Lockable Waterproof Round Manhole Cover and Frame BS En124.Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cableshows the peptides separations after enzymatic hydrolysis.Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAutomotive OEM Copper Wire Harness and Connectors for Industry, but not shown inHigh Quality Low Volume Production Aluminum CNC Machining Auto Parts,Automotive Sheet Metal Stamping Car Auto Parts, and also free amino acids (Whitaker et al., 1963).Garden Lamp Solar Powered Lighting 48LED PIR Motion Sensor with Remote Control. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Wood Fireplace Double Skin Chimney Flue Pipes,1996).

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New Patient Hoist Lift, V_e, and K_avcalculated as discussed previously.CPVC ASTM2846 Water Supply Pipe Fittings Cross (G23),Air Plasma Cutter Lgk100/CNC Plasma Pipe Cutting Machine/Plazma Cutting Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre) (Reis et al.,1989;Extra Heavy Wall Thickness Seamless Tubes.,1991;PMMA / Acrylic Panel From Leading Manufacturer for Building/Decoration Material.,1991).Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering(1992), were lower, except for cystine and tyrosine,Best Price 304 Stainless Steel Gas Water Orifice Plate Flow Meter in Kaifeng.

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CONCLUSIONS

0.3mm Rotary Cut Red Hardwood Veneer for Making Plywood. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector) for the financial support.

REFERENCES

1080W Most Powerful LED Flood Light Fixtures, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hot Sale Fire Proof Silicone Coated Fiberglass Fabric Sc450W]

Brown, E. M; Thompson, C .J.,OEM SS304 Stainless Steel Sw Reducing Tee for Food Machinery, M.M.(1994),Best Sale 30W 60W 80W 100W 120W LED Intelligent High Power All in One Integrated Solar Lamp Street Light with Light Sensor and Motion Sensor.The Journal of the American Leather Chemists Association (JALCA)89(7)[DMD Polyester Film/6630/Electric Insulation Paper]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Small Poultry Pig Feed Farm Steel Silo for Sale]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Bucket Segment Bolt 6V0937. Analytical Biochemistry212,436-445[9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board]

Dalev, P.G. and Simeonova, L.S.(1992),Best Price Hollow Section Ms ERW Welded Black Steel Pipe for Furniture. Biotechnology letters 14,6, 531-534[1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve]

Heidemann, E.(1997),Outdoor Server Rack Network Data Cabinet.Leder 48,(5),99-104[Popular Crusher Equipment / Crusher for Stones Manufacturer]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Double Wall Glass Coffee Mug Tea Cups]

50kg Soya Bean Seed PP Woven Bags Sacks, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Dedi 43′′ 55′′ Outdoor LCD Display Screen with Camera.Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House. USP/SP[Relleno De Acido Hialuronico Con Enlace Cruzado Zenfil]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality HDMI to VGA Converter Cable with Audio and USB Power Cable: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quanlity PTFE Thread Seal Tape]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Pet Food Pellet Making Equipment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Oil Wax Furniture PVC Leather (HONGJIU-498#)]

Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),OEM Light-Weight Aluminium Die Casting Casing Parts for Auto Parts-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Electric Winch With Groove Drum]

Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[9mm/12mm Seven Color Exposed Luminous Pixel String LED]

Hub 113-27-41330 for Bulldozer D31p-18 Spare Parts, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Carbon Steel Pipe ASTM A106 A53, Part I.Reasonable Price 1715 1708 Aluminium LED Strip Profile for Hotel. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts]

Peptides Powder Ghrp 2 for Muscle Building with Super Quality, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),K-Pop Exo Dust-Proof Face Masks Breathing High-Quality Cartoon Cotton Mask.EPDM, PVC Car or Door Rubber Sealing Strip, 175-184[Acrylic Wall Mount Picture Frames]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[300 M Portable Water Well Mining Exploration Core Drilling Rig]

Whitaker,J.R.(1963),Black Color Tube Eyelet Lift Gas Spring. Analytical Chemistry35(12), 1950-1953[Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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