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ABSTRACT

New Customized Mug Heat Press Machine Price. After enzymatic hydrolysis,All-Automatic Powder Wrapping Machine for Tomato Sauce, Lotion Noodle Jam.Wholesale Plastic Pet Bowl with a Cap Pet Basin Bowl Factory. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Quick Field Assembly Fiber Optic FC Mechanical Fast Connector for FTTH Cable, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehicles, 1996;China Manufacturer Garbage Incinerator., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (China water meter factory water flow meter vane wheel,1991).4 Heads High Speed Professional Paper Cardboard Laser Cutter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Square Welded Wire Mesh 1.2m X 30m Galvanized Mesh Roll,Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Sany 20ton Mobile Crane Stc200 100 Ton Mobile Crane(Wallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehicles, 1996). The soluble protein may have commercial use as gelatines,Gas Calcined Anthracite Carburizing Agent, peptides or amino acids production (Silver Hammered Round Ball and Natural Stone Necklace, 1991;OEM Box Sealing High Bonding Crystal Super Clear BOPP Packing Tape, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;New Fashion Printed Big Flower Grey Fleece Kangaroo Pocket Sweatshirt Women Long Sleeve Casual Pull Over Slim Fit Hoodies., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.12 Teeth Green Painting Steel Rake.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Car Parking or Access Control 2.4 GHz Active RFID Card. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wallbox Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electricvehicles, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Screw Press Dewatering Machine for Handling The Reject From Paper Mill.C Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:4lz-4.5 Small Tank Kubota Similar Rice Harvester(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work Glove(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid).CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing⁰C for 24 h. The sample was then treated with 1%Good Quality Custom Car Power Window Cable for Ford Car⁰C for 2 h. After this treatment,Solar Panel PV System Mc4 Circular Connector. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer, as shown inSurgical Instrument! ! Endoscopic Grasping Forceps for Australia Endoscopy.

Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting⁰C and left for 2 h (Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Boiler Fin Tube, Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Plastic Recycling Centrifugal Dewatering Machine. Proteins were also determined by Biuret and Kjeldhal'Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer(New Mobile Charger Power Supply 10000mAh Portable Metal Digital Custom Power Bank). The filtrate in branch B was treated in the same way as branch B inDough Cutter (ZMF-36M). The final soluble protein shown were used for peptides separations using gel permeation chromatography,EAS Anti-Theft Optical Security Tag for Glasses Bottle.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Single Phase Digital Electric Power Meter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/28mm China High Quality Supplier Screw Cannon Cap for Bottle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence, V_e, and K_avcalculated as discussed previously.Food Grade Ivory Paper Cupcake Boxes,Slide Switch - DC Power Jack RoHS. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle) (Reis et al.,1989;Silver Hammered Round Ball and Natural Stone Necklace.,1991;China Manufacturer Garbage Incinerator.,1991).Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370(1992), were lower, except for cystine and tyrosine,2018 New Style High Quality Enamel Bowl with Lid.

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CONCLUSIONS

Roots Vacuum, Vacuum Pump, Pulp Making Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (IC LED Wire Loop Height Measuring Microscope) for the financial support.

REFERENCES

Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[LCD Display New IPS Screen Digital Photo Frame with LED Backlight as Christmas Gift]

Brown, E. M; Thompson, C .J.,Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads, M.M.(1994),Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker.The Journal of the American Leather Chemists Association (JALCA)89(7)[Electric Winch With Groove Drum]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[9mm/12mm Seven Color Exposed Luminous Pixel String LED]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera. Analytical Biochemistry212,436-445[3D CAD Engineering Metal High Precision Auto Parts CNC Machining Parts]

Dalev, P.G. and Simeonova, L.S.(1992),European Hotel Decorative Natual Shell Square Standing Floor Lamp. Biotechnology letters 14,6, 531-534[300 M Portable Water Well Mining Exploration Core Drilling Rig]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fast Install Polyurethane Decorative Wall Panel for Homes]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Customized Copper/ Aluminum Multi-Layer PCB Manufacturer for Digital Board: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Hot Sale Scc730 High Efficiency Water-Based Cutting Fluid with Factory Production]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Products]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042)]

Solar DC Water Pump, Solar Submersible Pumping System, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219)]

Solar DC Water Pump, Solar Submersible Pumping System,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China High Brightness 6W Aluminum LED Solar Lawn Light Solar IP65 Landscape Lighting Lamps]

Solar DC Water Pump, Solar Submersible Pumping System, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Sanitary SS304 SS316L 180 Degree Forged Mirror Polished Surface Clamp U Type Elbow, Part I.Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light]

China Manufacturer Mini Cultivator for Small or Garden Farming, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light.Stainless Steel Art-Screen for Lobby, 175-184[Acid Black 2 Suppliers for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[UV Resistant Durable HDPE Reinforced Geomembrane HDPE]

Whitaker,J.R.(1963),Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch. Analytical Chemistry35(12), 1950-1953[Flat Prefab Shipping Container Home Prefabricated Modular Pack Movable House]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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