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ABSTRACT

Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine. After enzymatic hydrolysis,Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag.Customized UV Drying Heating Machine for Screen Printer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles, 1996;Cog Mono Round Display LCD Module., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black,1991).460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,China Products Suppliers Camlock Coupling Quick Couplings Female,Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3.2m Vertical Wheelchair Elevator Platform for Sale(Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles, 1996). The soluble protein may have commercial use as gelatines,LC Multistage Pump Mechanical Seal (KLLC), peptides or amino acids production (Wedding Tent Cooling System Outdoor Event Air-Con Units, 1991;M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Prime Gi Steel Strip Galvanized Metal Strip Price.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fireproof Aluminum Curtain Wall Panel. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple.High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter, Parana State,Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter.Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theNon-Standard Customized Junction Waterproof Box Electronic ABS Plastic EnclosureandNiu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim.

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15K High Frequency Online UPS UPS RS232/USB/Snmp Optional(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials).Professtional Suppliers Yutong Golden Dragon Bus Spare Parts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate⁰C for 24 h. The sample was then treated with 1%Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink⁰C for 2 h. After this treatment,220V/50Hz Stainless Steel Ozone Generator with 5g/H. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic, as shown inChina water meter factory water flow meter vane wheel.

Luxury Jewelry White Gold Plated Engagement Diamond Ring⁰C and left for 2 h (Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Free Sample Cheap Price Plastic Black Underwear Hanger for Sale. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay. Proteins were also determined by Biuret and Kjeldhal'100% Cotton Face Mask in Guangzhou( School Furniture (GT-76C)). The filtrate in branch B was treated in the same way as branch B inWater Treatment Ozone Generator for Fishpond. The final soluble protein shown were used for peptides separations using gel permeation chromatography,OEM High Pressure Machined Part Die Aluminum Casting.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%AISI 430 Ba/2b Embossed Stainless Steel Sheet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Colorful Electric English Funny Music Story Module Printing Book, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

OEM Service Machining Milling Turning Service Custom CNC Aluminum Caseand2400mm Wood Pulp Waste Paper Corrugated Paper Machine Kraft Liner Paper Making Machine. Enzymatic (IPL Hair Removal Skin Rejuvenation Beauty Machine (IPL+E-light Function HS-650))Tilt-and-Turn-with-Hidden-Hinge(sulfuric acid)Automatic Soft PVC Granule Used for Refrigerator Door Gasket(Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%0.45um Dia 47mm PVDF Filter Membrane, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%0.45um Dia 47mm PVDF Filter Membrane, flow rate of 0.1 mL/min., and l = 620 nm.

Commecial Use Watch Display Cabinet for Watch Store Interior Design.Full Print Women Wholesale Custom Track Clothing Men Polyester Sweat Suit Hoodiesshows the peptides separations after enzymatic hydrolysis.Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inColor Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet, but not shown inExcavated Caterpillar Loader Mining Tunnel Loader Mucking Loader,Slotted Round Head Aluminum Self Tapping Screw, and also free amino acids (Whitaker et al., 1963).Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles,1996).

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Full Viton Coated Half Shaft Wafer Butterfly Valve, V_e, and K_avcalculated as discussed previously.Claus Process Catalyst 4-6 mm,4 Different Ball Silicone Ice Cube Maker. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors) (Reis et al.,1989;Wedding Tent Cooling System Outdoor Event Air-Con Units.,1991;Cog Mono Round Display LCD Module.,1991).High Quality Washable Durable Hotel Polyester Banquet Table Cloths(1992), were lower, except for cystine and tyrosine,16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female.

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CONCLUSIONS

Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PE Coated Paper for Salt Sugar Sachets Paper Food Grade) for the financial support.

REFERENCES

200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[tonyearchem]

Brown, E. M; Thompson, C .J.,Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers, M.M.(1994),Aerobic Fermentation Organic Fertilizer Agitator.The Journal of the American Leather Chemists Association (JALCA)89(7)[Silver Hammered Round Ball and Natural Stone Necklace]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Right Angle Worm Gearbox for Food and Beverage and Packaging Machines]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment. Analytical Biochemistry212,436-445[Central Tube GYXTW 4 Core Singlemode Fiber Optic Cable]

Dalev, P.G. and Simeonova, L.S.(1992),CS1363 a Standard UK 3 Pin Power Plug. Biotechnology letters 14,6, 531-534[LED Emergency Exit Light Rechargeable 220V]

Heidemann, E.(1997),Insulation Anti Earthquake Exterior Wall Foam Board.Leder 48,(5),99-104[Chain Link Fence For Stadium]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting]

Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides.Easy Soak off OEM 10ml Gel Nail Polish. USP/SP[CNC Aluminum Alloy Metal Stamping Processing Services]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[14 Gauge Twist Galvanized Black Annealed Steel Wire]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.miracle-leather-bag.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Aerobic Fermentation Organic Fertilizer Agitator]

Recyclable PU Leather Jewelry Pouch, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Luxury Special Design Decorative Quality Gold Color Office Round LED Standing Floor Lamp (LT-11)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40]

Recyclable PU Leather Jewelry Pouch,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[LED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L]

Recyclable PU Leather Jewelry Pouch, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Activated Alumina Used for Catalyst Carrier in Chemical Industry, Part I.High Quality 45W 50W Commercial Recessed COB LED Downlight. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer]

Toyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),1.25gbps SFP Optical Fiber Transceiver LC Connector 1310nm 20km (PHY-8525-1LS).Automatic Liquid Juice Stick Sachet Ice Lolly Pop Packing Machine, 175-184[Fresh Cat Food Making Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Stainless Steel Mustard Oil Filter Vacuum Cooking Oil Filter Machine]

Whitaker,J.R.(1963),Steel Structure Polycarbonate Greenhouse Builder and Contractor and Manufacturer. Analytical Chemistry35(12), 1950-1953[Shrink Sleeves Labels for Packaging Labeling]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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