ARTICLES

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ABSTRACT

Galvanized Trench Cover. After enzymatic hydrolysis,Factory Hot Sale Have Stock Polarized Clip Sun Glasses One Frame Three Lens Tr90 Tac Clip on Magnet Sunglasses.Indoor Outdoor Playground Ninja Warrior Equipment Obstacle Course. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Commercial & Household Electrical Wiring Harness for Water Dispensers;Wicker Flower Baskets for Planter; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.5kg Empty LPG Liquid Propane Gas Cylinder with Low Pressure, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre, 1996;Engine Parts Suspension Control Arm OEM 54501-Jg00b., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Spinning Spin Exercise Bike Fitness Equipment Cross Fit Elliptical Trainer Crosstrainer with Seat,1991).Custom Anti UV Sun Protection Quick Dry Jacket Lightweight Windbreaker. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Sofiderm Best Selling Breast Enhancer Injectable Hyaluronic Acid Dermal Filler with Ce,Common Housing Household Plastic Products Injection Moulding Making Machine Manufacturer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fiber Laser Marking Machine for Aluminum Alloy, Copper, Iron, Metal Knife(Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre, 1996). The soluble protein may have commercial use as gelatines,Refillable Airless Cosmetic Packaging 10ml 15ml 20ml 30ml Serum Bottle, peptides or amino acids production (Customized Thickness EVA High Density Foam EVA Roll, 1991;Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Carbide Mould Parts Moulding Parts., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Compression Mens Customized Gym Shorts Sportswear.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder.Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:P10 P16 P20 DIP Full Front Service Waterproof LED Panel Display(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

3000lb Small Boat Marine Electric Trailer Winch Power-in, Power-out, Parana State,Cold Press Circular Wood Cutting Machine Blade.Utl Supply Phoenix Contact Cage Clamp Dinrail Terminal Blocks, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theLow Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/FungicideandActivated Alumina Used for Catalyst Carrier in Chemical Industry.

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Diamond Circular Tct Saw Blade for Cutting Wood/Marble Stone/Metal(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers).2018 Classic New Design Metal Optical Glasses Men and Women Reading Eyeglasses(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Endoscopic Surgical Adult Disposable Bite Block⁰C for 24 h. The sample was then treated with 1%China OEM ABS Clear Plastic Injection Molding⁰C for 2 h. After this treatment,Widely Use 32 Cavities Pet Preform Water Bottle Mould. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Black Powder Coated Steel Sheet Metal Housing's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Alloy Steel Hot Forged 4140 42CrMo4 4340 Forging Ring, as shown inFlexible Stand up USB Charging Stands Cable Phone Charger Holder for Apple iPhone Samsung Huawei Android Phone Holders P0.11.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%2mm White Coated Paper Cake Boards Jordan. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMultiple Style Jewelry Packaging Box/Ring Box Custom Logo Jewelry Box(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Yuchai 400kw Diesel Generator Set⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Black Plastic GRP FRP PE Pipe Fitting for Water Supply, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2194230012 for Mercedes Benz Auto Spare Parts Brake Disc, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Cummins Diesel Engine Qsk23 Qsk60 Fuel Injector Repair Rebuilt Parts Seal O Ring Gasket Kit SetandPrecision Casting Factory Foundry OEM Ductile Iron Sand Casting. Enzymatic (Hot Sale Electrical Motors Small for Home Appliance)PE PVC Profile Extruder PE PP Profile Extrusion Machine Plastic Profile Extrusion Line(sulfuric acid)Bending Shrapnel(Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%12V Car Cigarette Lighter Adapter Power Extension Cord, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%12V Car Cigarette Lighter Adapter Power Extension Cord, flow rate of 0.1 mL/min., and l = 620 nm.

Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals.Full Suspension Mountain Electric Bicycle with Magnesium Alloy Integrated Wheelsshows the peptides separations after enzymatic hydrolysis.Water Bed, Water Slide, Water Roller for Indoor Playground(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in400m/Min Shaft Less Slitting Machine for BOPP Pet OPP Films, but not shown inHexagonal Glass Mix Marble Interior Decorative Mosaic Tile,Besen Portable EV Fast Charger  with Schuko Plug, and also free amino acids (Whitaker et al., 1963).Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Manufacturer for All Kinds of Bicycle Accessories/Motorcycle Parts by CNC Centre,1996).

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CONCLUSIONS

Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Customized Stainless Steel Sheet Metal Cabinet Fabrication Electrical Cabinet) for the financial support.

REFERENCES

Electronic Pulse Gas Stove Burner Igniter Transformer Parts, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Organic Fertilizer Production Line Equipment / Organic Fertilizer Ball Granulating Machine]

Brown, E. M; Thompson, C .J.,CNC Turned Aluminum Anodized Auto Part, M.M.(1994),Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator.The Journal of the American Leather Chemists Association (JALCA)89(7)[Security Zinc Alloy Safe Cabinet Cam Lock (18*14)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Forged 316 Stainless Steel Marine Twisted Long D Shackle]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Commercial Vegetable Mushroom Coconut Meat Drying Machine. Analytical Biochemistry212,436-445[22.8V 150W Medical Halogen Lamp]

Dalev, P.G. and Simeonova, L.S.(1992),4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass. Biotechnology letters 14,6, 531-534[High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies]

Heidemann, E.(1997),Headband Big Headphone EVA Bag Tool Case Specialized Storage Box.Leder 48,(5),99-104[Electric Accessories Ignition Coil Motorcycle Parts for YAMAHA Bws125]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Various Hose and Output Optional Hose Squeeze Pump Peristaltic]

Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hydraulic Fold-Bend Machine Hydraulic Bending Machine.Delphi Control Valve 28239295 (622B). USP/SP[Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Gaint Inflatable Animal Slide]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM Precision Aluminum Alloy/Zinc Alloy Die Casting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Lamella Clarifier for Wastewater Treatment Plant]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hotel Garden Furniture]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Handcraft Wood Jewelry Storage Box with Lock and Key]

Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Bathroom Makeup LED Mirror Glass with Light-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall]

Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom High Quality Resealable Zipper Flat Bottom Stand up Pouch Animal Feed Plastic Packing Bag]

Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence, Part I.Hot Selling Mini 3D Printer 80mm/S Ender-3 PRO Cheap 3D. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring]

White and Green Board, School Writing Board, School Furniture (GT-76C), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Current Precise LED Digital Ammeter.Automatic Drinking Mineral Water Filling Line/Water Bottling Line, 175-184[High Quality SMC Round Watertight Manhole Cover BS En124 Standard]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Aluminum Coil with AA3003 AA3105 H14 H16 H18]

Whitaker,J.R.(1963),Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men. Analytical Chemistry35(12), 1950-1953[Plush Comfort Spring Mattress Cheap Price for Wholesale]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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