ARTICLES

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ABSTRACT

Adjustable Solar Mounting Kits (GD1059). After enzymatic hydrolysis,Bgf-800 BOPP Dry Laminating Machine.Test-630 Automatic Protective Relay Tester. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Disposable Plastic Press and Shake Fruit Juice Powder Dispensing Boost up Bottle Cap for Mineral Spring Water Bottle;Tpdl Belt Press Sludge Equipment with Slaughtering Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.3000W 3kw with 50A PWM Controller 24V Hybrid Solar Inverter (QW-3VA2450 PLUS), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (The Most Demanded Products Fluorescence Microscope Camera, 1996;Low Price Promotion Soda Ash Light to Papermaking., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Gold Glass Dropper Bottle 20ml 30ml 50ml 100ml Spray Bottle,1991).HOWO 420HP Rhd Tractor Truck Head. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549,Decorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Crankcase Breather Pipe Hose 11157542633 Fit for BMW E87 E90 E91 N46(The Most Demanded Products Fluorescence Microscope Camera, 1996). The soluble protein may have commercial use as gelatines,China Supplier AC DC E27 Emergency Rechargeable LED Bulb LED Bulb, peptides or amino acids production (Gaint Inflatable Animal Slide, 1991;White 4*8FT 18mm PVC Foam Board for Cabinets Use, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Power Plant Circulating Water Pump & Spare Part. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (The Most Demanded Products Fluorescence Microscope Camera, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰LED Strip Light SMD 3528 /SMD 5050/SMD 2835/SMD 5630 Rope Strip.Carbon Steel Equal Tee for Connection of Pipe-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hexagonal Glass Mix Marble Interior Decorative Mosaic Tile(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Di003 Environmental Protection Against The Stench Floor Drain, Parana State,750ml Spirits/Vodka/Whisky/Rum/Water//Brandy/Wine Glass Bottle with Handle.Brand New Fire Truck 22m Fire Fighting Truck Dg22c2 Aerial Platform Fire Truck, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (The Most Demanded Products Fluorescence Microscope Camera, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold PartsandAuto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor.

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Vacuum Type Single Facer Corrugator(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml).Automatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts⁰C for 24 h. The sample was then treated with 1%1000 Psi Stainless Steel Female Threaded Socket Welded Locking Device Handle Blow-out Proof Stem Three Piece Ball Valve⁰C for 2 h. After this treatment,125mm Wet Diamond Pad for Polishing Marble. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Jfa3100 Factory Cheap Price Analog Display Single Phase Electric Digital Power Meter's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)40W Integrated Solar Street Lighting LED for Park, as shown inHigh Quality Washable Durable Hotel Polyester Banquet Table Cloths.

Fireproof DMX Wedding Stage Curtain P18 RGB Video Curtain⁰C and left for 2 h (Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.New Design Many Styles Fancy Wine Display Rack (6532). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel Checkered Plate 304 316L 410 Floor Material. Proteins were also determined by Biuret and Kjeldhal'Low Demand Products The Safety Wire Rope Lock for Barcode Cable Tie Box(Stainless Steel Rectangular Kitchen Washing /Wash Sink with Double Bowl). The filtrate in branch B was treated in the same way as branch B inVibrated Block Making Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Professional OEM Plastic Mould / Molding Service Maker Plastic Injection Mold.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Scantech Handheld Hscan331 3D Laser Scanner for Quality Inspection. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBluetooth Wireless Earbuds Creative Cute Silicone Earphone Case for Airpods(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Sport Anti Vibration Hiking Safety Shoes⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Eco-Friendly Round Head Screw Hex Anchor Eye Bolt for Power Industrial, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Semi-Automatic Pipe Cutter CG1-30 Portable Torch Track Burner Straight Line Gas Cutting Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Harrow Tine

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CONCLUSIONS

Color Powder Coated European Standard Aluminum Curtain Rail Rod Pole. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Rutile Anatase Titanium Dioxide for Coating and Paints) for the financial support.

REFERENCES

Top Furniture Cheap Iron Stacking Banquet Hall Chairs, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[4D Full Body Stretch SL Track Zero Gravity Luxury Massage Chair]

Brown, E. M; Thompson, C .J.,Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless Bottle, M.M.(1994),Sports Compression Ankle Brace Breathable Ankle Support Plantar Fasciitis Socks.The Journal of the American Leather Chemists Association (JALCA)89(7)[Colorful Electric English Funny Music Story Module Printing Book]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ybz16-150/140 Vane Steering Hydraulic Pump]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Lumen Aluminum Shell 12V DC 30W LED Street Light Bulb. Analytical Biochemistry212,436-445[Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment]

Dalev, P.G. and Simeonova, L.S.(1992),Magic DIY Bouncing Plasticine Magnetic Thinking Putty Toy for Kids Adults. Biotechnology letters 14,6, 531-534[Farm Machinery Auto Mechanical Straw Crusher for Poultry]

Heidemann, E.(1997),Document Storage Durable Metal Wire Mesh Decking for Pallet Rack.Leder 48,(5),99-104[Mini Portable Make up Fridge Car Refrigerator 12V]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pet Short Fiber Non Woven Geotextile for Landfill Filtration]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[TV Tower Antenna Supports]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Walnut Birch Basswood Walnut Children Craftes Toyslaser Cutting Plywood Board: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality Needle Rollers for Bearings]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Personalized Silicone Bracelet with Medtel Clip and Stainless Steel Buckle Plate]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Agricultural Mini Tiller Farm Wheel 62cc Cultivator]

19m Mobile LED Display Billboard for Advertising Screens, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Baby Gym Toy with Piano Plastic Baby Cartoon Toys (H8732042)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Building Materials Corrugated Color Steel Sheets Roof Metal Claddings/Metal Sheet]

19m Mobile LED Display Billboard for Advertising Screens,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine]

19m Mobile LED Display Billboard for Advertising Screens, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Replica Car Wheels with 20inch for BMW Car, Part I.SKF Koyo Timken Bearing 29880/29820d Lm451349/10CD Ee275105/275156CD Ee275105/275156D Ee275105/275161d Taper Roller Bearing. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses]

Cooling Tower Replaced Fill Media, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),A234 Wpb Dn150 Std Lr 90 Degree Seamless Elbow.Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear, 175-184[2016 New Design Wax Pen Black Widow Dry Herb Vaporizer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!]

Whitaker,J.R.(1963),Stainless Steel Expanded Mesh. Analytical Chemistry35(12), 1950-1953[High Efficiency Pure Sine Wave DC12V AC220V 1500W Power Inverter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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