ARTICLES

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ABSTRACT

Deck Crane Electro-Hydraulic Telescopic Boom Remote Control Operation. After enzymatic hydrolysis,Dust Hood Fume Extraction Systems for Robotic Laser Cutting.IP40 M8/M12/M16/M23 Male Waterproof Cable Circular Industryconnector. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Experienced and Professional Plastic Helmet Mold Manufacturer, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Private Label OEM Best Cute Waterproof Lip Pluming Gloss, 1996;Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Good Excellent Rubber Yoga Mat Making Machine / Rubber Tile Making Machine/Rubber Floor Press,1991).High Quality 10W iPhone Case Laptop Metal Sheet LED Laser Printing Marking Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,IN-505 Cheap ABS Plastic Medical Filing Drawers Bedside Cabinet,Tent Windows Use China Made PVC Transparent Clear Mesh Tarp,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,13 Oz Flex Banner PVC Frontlit Banner Material(Private Label OEM Best Cute Waterproof Lip Pluming Gloss, 1996). The soluble protein may have commercial use as gelatines,Custom Black Zipper Puller with Metal Slider, Design Engraved Logo Zipper Puller, peptides or amino acids production (3DTALK FAB460 Large Printing Size 420*420*460mm Industrial Level FDM 3D Printer with Multiple Consumables, 1991;Chubby 163cm Fat Big Ass Female Real Sex Doll for Man, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Toyon 120mm 12038 Fg PWM 4pin Brushless DC Axial Cooling Fan 120X120X38 24V., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.10mm AISI304 Carbon Steel Balls Motor Bearing for Slide.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Different Size of Ring NdFeB Magnet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Private Label OEM Best Cute Waterproof Lip Pluming Gloss, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Investment Precision Casting for Valve Body with Machining.Unique Best Quality Copper Home Alcohol Distiller for Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Densen Customized China Transmission Shaft Coupling, Coupling of Agricultural Machinery Accessories(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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SMT Mini Desktop Visual Chip Mounter Tp210+ (TORCH)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (P4.75 Indoor Single Dual Color Advertising LED Display Billboard).Auto Spare Parts Ignition Coil 22448-2y001(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3D Good Quality Laser Engraving Machine for Souvenirs Shop⁰C for 24 h. The sample was then treated with 1%Built in Controller! Magic Pie 24V, 36V, 48V 250-1000W / Electric Bike Conversion Accessories⁰C for 2 h. After this treatment,3.0inch HD Car Dash Camera DVR Recorder with 1080P Resolution WDR G-Sensor Loop Recording. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Reverse Flow Configuration Axial Fan's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Handcraft Wood Jewelry Storage Box with Lock and Key, as shown inChina Cheap Newest 70*77cm XPE Foam 3D Wallpaper.

TBR SBR Linear Rail Support Bearing Unit Aluminum Linear Rail Unit⁰C and left for 2 h (500kg Capacity 14m Height Semi Electric Scissor Lift). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Single Spindle Micro CNC Lathe Ck0640. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Various Styles Indoor Fire Proof Eco Click Vinyl Spc Flooring. Proteins were also determined by Biuret and Kjeldhal'Pipe Thermostat with Sensor Ce Certificate(Geotextile for Airport Runways/High Way). The filtrate in branch B was treated in the same way as branch B inModern Design Steel Structure Layer Chicken Cage Poultry Farm House. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Frosted Flakes Plant/Gluten Free Bulk Breakfast Cereal Corn Flakes Production Line.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ce Wood Grain 0.2mm~0.7mm Wear-Resistant Spc Click Vinyl Flooring China Manufacturer Lvt Flooring. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNitinol Disposable Guide Wire(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fashion Wool Felt Lady Cloche Wide Brim Floppy Hat with Felt Bow⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Crane Hook Qtz250 (7032) with 70m Jib Length, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Manufacturer Price Home 24V 250W Mono Poly PV Solar Cell Panel for Solar System, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Single Cheap Custom Festival Woven RFID Fabric Wristband for Events.Hot Sale Auto and Type Customized Car Paint Spray Booth by Ceshows the peptides separations after enzymatic hydrolysis.Children Gift Outdoor Wooden Rocking Horse Toy(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Sensitivity Ground Metal Detector Searching for Treasure, but not shown inHigh Quality Anti-Corrosion Fiberglass Sheet and Roll,Atlas Copco Compressor Spare Parts Service Kit Electronic Drain 1622855181 2901146500 Ewd330m Ewd330, and also free amino acids (Whitaker et al., 1963).TBR Ceramic Roller PLC Control Double Heating Section Tempered Glass Making Furnace for Solar Glass Appliance Glass. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Private Label OEM Best Cute Waterproof Lip Pluming Gloss,1996).

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XCMG Official Manufacture Tractor Tractors Mini Tractor Construction Equipment Bulldozers Backhoe Wheel Loader Towable Backhoe  Site Dumpe, V_e, and K_avcalculated as discussed previously.Outdoor High Neck Kids Boots Casual Rubber Men Work Boots,Best Selling Sequin Pillows / Reversible Cushions Square (YFC0061). The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Cheap Price Paper Straws Individually Wrapped Drinking Straw) (Reis et al.,1989;3DTALK FAB460 Large Printing Size 420*420*460mm Industrial Level FDM 3D Printer with Multiple Consumables.,1991;Single Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display.,1991).Hr17 Series 160A, 250A, 400A, 630A 3p Fuse Switch Disconnector(1992), were lower, except for cystine and tyrosine,2019 Anti Theft Credit Card Protector Aluminum RFID Blocking Wallet.

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CONCLUSIONS

CNC Grinding Machining Parts High Precision. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom 500ml Travel Mug Double Walled Vacuum Flask Water Bottle for Gift and Outdoors) for the financial support.

REFERENCES

High Quality Cheap Price Disposable IV Infusion Set with Precise Filter, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Luxury Electroplating 3D Printing Pattern Round Air Cushion Cosmetic Packaging]

Brown, E. M; Thompson, C .J.,Clear Drug Packaging Custom Printed Recloseable Medicine Plastic Bag, M.M.(1994),GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve.The Journal of the American Leather Chemists Association (JALCA)89(7)[Ivd Component PP Barbed Threaded Connector]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Disposable Clear Take Away Plastic Salad Bowl Forming Machine. Analytical Biochemistry212,436-445[Outdoor Server Rack Network Data Cabinet]

Dalev, P.G. and Simeonova, L.S.(1992),Blank PVC Card 13.56MHz/ 125kHz Contactless Smart Card for Access Control. Biotechnology letters 14,6, 531-534[Factory Custom Round Aluminum Tube 5154 5454 5652 5086]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Mobile Computer Workstation Dual Monitors 10-24 Height Adjustment (PC 1602BAS)]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hydraulic Three Roll Mill with Hydraulic Squeeze Pressing system]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-OEM Wld8200 Infrared Lamp Spray Paint Booth China Supplier for Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Medical Packing Self Sealing Sterilization Pouch]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[3d Pvc Foam Board]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cheap Price Roof Wall PU Sandwich Panel Press]

Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Selling House Floor Cleaning 360 Spin Mop-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Reasonable Price 1715 1708 Aluminium LED Strip Profile for Hotel]

Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[380V 3.0HP Peristaltic Pump Swimming Pool Pump]

Yutong Bus Parts Ceiling Diffuser Vent Air Louver Vent Air Diffuser Vent Air Diffuser Ceiling Vent Slot Diffuser Vent, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Srfloor Plastic Flooring Interlock Suspended Outdoor PP Tiles, Part I.Advanced Unlocking Tool for Locksmith. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Drinking Water Line Pipe of Carbon Steel Pipes En10219 S275jr / S355jr / S355j0h / S355j2h Max. 18m Per Each Length]

High Precision ISO9001 Grinding Milling Turning Motor Cover Manufacturer Precision Parts with Auto Spare Racing Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Fashion Design Sleeveless Fitness Wear.DC Colorful Battery Operated Small Hand Fan for Outdoor Use, 175-184[Villa Big Size U Shape Leather Living Room Sofa on Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[45ton Proof Load Testing Water Bag for Life Boat]

Whitaker,J.R.(1963),Hsc Heavy Duty 5 Ton Chain Pulley Block. Analytical Chemistry35(12), 1950-1953[Chubby 163cm Fat Big Ass Female Real Sex Doll for Man]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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