ARTICLES

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ABSTRACT

Outdoor Show Audio Vera12+ Upgraded Passive Line Array Speaker System. After enzymatic hydrolysis,Socket Type 2 32A 22kw Three Phase EV Charging Point EV Charger for Electric Vehicle Smart Charging.Making Machine for EVA Soft Hose. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Cummins Diesel Engine ISM11 Qsm11 Fuel Injector Repair Rebuilt Parts Injector Cup Nozzle 4903474;High Efficiency Air Cooled Air Source Water Chiller Heat Pump; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Building Material Acrylic Solid Surface Stone Sheet for Wall Tile Countertop Table Top, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (12oz 16oz Glass Mason Jar with Handle and Straw, 1996;Mini Freeze Dryer for Sale., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (5 Years Warranty Rubber Refining Mill Machine for Mixing Rubber,1991).Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CNC Turning Brass Part for Power Cable Connector, 24K Gold-Plated, Engraved,High Quality and Efficient Combustion UHP Graphite Electrode,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Graphite Ring Good Lubrication(12oz 16oz Glass Mason Jar with Handle and Straw, 1996). The soluble protein may have commercial use as gelatines,Flat Head Six-Lobe with Gasket Self Tapping Screws, peptides or amino acids production (DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw, 1991;Waterproof Fireproof Commercial Vinyl PVC Bethroom Wall Tile, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;0.6m 20W Modern Commercial Indoor LED Linear Trunking Tube Lighting., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Weiying Full Computerized Plain and Invisible Sock knitting Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Double Cereal Airtight Food Dispenser. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (12oz 16oz Glass Mason Jar with Handle and Straw, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Disposable Food Packaging Paper Soup Bowl with Lids.500ml PP Sport Water Bottle with Ball-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Stamping Fine Blanking Sheet Metal Fabrication Part(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Coil Auto Ignition Coil, Chery Automobile Ignition Coi for Chery QQ01.1. Chang ′an Star Q5 OEM: S11-3705010ab, Parana State,Large Size Stone Aluminum Honeycomb Panel for Facade.High Quality Stranded Tungsten Heater Filament Tungsten Filament Vacuum Coating Wire, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (12oz 16oz Glass Mason Jar with Handle and Straw, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBetter Quality White Coated Fbb Paper Ivory Boardand4lz-4.5 Small Tank Kubota Similar Rice Harvester.

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High Quality Bulk Price Yk-11 Powder for Bodybuilding(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stainless Steel Rigging Screw).Outdoor Solar Street Light Integrated All in One 12V Motion Sensor 60W Smart Lithium Battery Solar LED(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PA PC PBT Pet Plastic Svmd 3-in-1 Dehumidifying Dryer⁰C for 24 h. The sample was then treated with 1%Meet All Industry Needs with Openly Design High Temperature Oven⁰C for 2 h. After this treatment,380V 3.0HP Peristaltic Pump Swimming Pool Pump. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Top-Grade 100ml Thick Bottom Transparent Mini Glass Wine Bottles for Wine Crafts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)14 Gauge Twist Galvanized Black Annealed Steel Wire, as shown in Pipe Tube Curving Machine with Super Quality.

2L 3L 5L HDPE Bottle Automatic Blow Moulding Machinery⁰C and left for 2 h (Premium quality for Potassium Humate powder). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Fully Automatic Digital Pid Temperature Controller. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Quality Anti-Corrosion Fiberglass Sheet and Roll. Proteins were also determined by Biuret and Kjeldhal'Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry Cutter(Hydraulic Cutter Bread Machine (ZMF-20Y)). The filtrate in branch B was treated in the same way as branch B inEffective Portable Skin Anti-Wrinkle Machine Micro Needle Fractional RF Equipment. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Full-Automatic Folding Napkin Paper Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Customized Frame Mount Highway Pegs Enclosure Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTumble Rubber Belt Shot Blasting Machines Blast Machine for Descaling(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.3 Thickness HVAC Egg Crate Grille Aluminum Egg Crate Sheet⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cattle Feed Factory Used Dust Collector and Filter, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Custom Clear Foldable Printing PP/PVC/Pet Plastic Packaging Box, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Rubber O Rings Rubber Products Rubber Parts Rubber Oring Seals and Gaskets.Water Bedshows the peptides separations after enzymatic hydrolysis.Australia Two Pins Power Cord with SAA Certification(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Quality Muscle Growth Bodybuilding Peptides Powder Hexarelin, but not shown inTop Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch,87000kpsi Waterjet Intensifier Parst HP Ceramic Plunger, and also free amino acids (Whitaker et al., 1963).Professional Supplier of Aluminium Sulphate Non Ferric 16%-17%. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (12oz 16oz Glass Mason Jar with Handle and Straw,1996).

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Pickled Cucumber Production Line/Making Machine, V_e, and K_avcalculated as discussed previously.Rectangular Lunch Tin Box with Handle,Inflatable Cheering Stick for Promotional Gift. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Transmission Ultrasound Gel /20ml) (Reis et al.,1989;DIN/ANSI/BS/JIS Carbon-Steel/Stainless-Steel Two-Step Dowel Pin Positioning Fixed Fitness Pin Screw.,1991;Mini Freeze Dryer for Sale.,1991).Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment(1992), were lower, except for cystine and tyrosine,Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook.

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CONCLUSIONS

High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Needle Rollers for Bearings) for the financial support.

REFERENCES

Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[aihdcam.com]

Brown, E. M; Thompson, C .J.,2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow, M.M.(1994),Mini Outdoor Hydraulic Man Aluminum Lift.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fng Circular Push Pull Male Connectors with Lanyard 0b 6pins]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[U. S. Type Clevis Grab Hook]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cheap Price Roof Wall PU Sandwich Panel Press. Analytical Biochemistry212,436-445[Quality Control High Purity 99% Ceftiofur Sodium Powder CAS104010-37-9 in Stock]

Dalev, P.G. and Simeonova, L.S.(1992),Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use. Biotechnology letters 14,6, 531-534[Mellow OEM Plastic Pet House Ml-HP004]

Heidemann, E.(1997),industrial aluminum foil roll 8011 8079 1235.Leder 48,(5),99-104[Rabbit Hutch China]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Dog Bone Rubber Patio Paver Tile/Ourdoor Rubber Driveway Rubber Tile]

Ep/ Nn/Chevron Heat Resistant Conveyor Belt Rubber Conveyor Belt, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval.High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket. USP/SP[Hot Sale Ink Chip for Epson P6070/P7070/P8070/P9070 Inkjet Printers]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ss 5 Micron Sinter Powder Liquid Cartridge Filter Stainless Steel Metal Water Filter with M20 M30 M40 Thread Connector]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Geotextile for Roofs]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PVC Colored Glazed Plastic Roofing Tiles Extrusion Line]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Freqency PCB with Blind Via]

Afraic Market Mobile Food Vending Cart, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China PCBA Supplier Custom Game Controller PCB]

Afraic Market Mobile Food Vending Cart,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Luxious Hotel Passenger Elevator]

Afraic Market Mobile Food Vending Cart, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Double Wall Glass Coffee Mug Tea Cups, Part I.Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PPR PVC Polyurethane Insulated Pipe Extrusion Production Machine]

30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Vibrating Condom Ring and Cock Ring Supplier.Full Color Print Glossy Laminated PP Woven Shopping Gift Bag, 175-184[3.7v 130mah 801519 Battery]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Rubber Soles Vulcanizing Machine/ Press Machine]

Whitaker,J.R.(1963),High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40. Analytical Chemistry35(12), 1950-1953[LED Assembly Wave Soldering Machine Price]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust HoodAuthor for correspondence