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ABSTRACT

P4.75 Indoor Single Dual Color Advertising LED Display Billboard. After enzymatic hydrolysis,New Smart Housing Price Motion Sensor All in One Integrated Solar LED Street Light IP65.Plug-in Assembly Type Straight Through Joint and Insulation Joint. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Quilting Machine for Muti- Needle Mattress Quilting Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Single-Screw Plastic Extruders Machine, 1996;Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quanlity Colorful Aluminum Spacer/Standoff,1991).5g OPP Silica Gel Desiccant. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Electric Winch With Groove Drum,Lds1500 Dome Construction Used Small Peristaltic Type Squeeze Concrete Pump,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Truck Air Dryer cartridge 14bar(Single-Screw Plastic Extruders Machine, 1996). The soluble protein may have commercial use as gelatines,Lost Wax Casting Investment Casting Marine Hardware Horizon Claw Anchors, peptides or amino acids production (3mm White Waterproof PVC Foam Board, 1991;Educational Equipment Smart Multi Touch Interactive White Board for School, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Manufacturer New Styel 1500W 60V Adult Electric ATV/ Quad Bike., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Women Party Jewelry 45cm Sequins Choker.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-New Bathroom Vanity Hot Selling USA Style Bathroom Cabinet Vanity. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Single-Screw Plastic Extruders Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality Hot Sale PVC/ UPVC Water Drain Irrigation Supply Pipe.High Freqency PCB with Blind Via-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Strong Aluminum Armrest Reclining Hydraulic Barber Chair(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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4.2A Type a Type C Pd QC3.0 USB Charger(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes).Ferrous Sulphate Monohydrate 30%Min Factory Wholesale(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Small Portable Scooters Folding Electric Scooters for Adults⁰C for 24 h. The sample was then treated with 1%Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant⁰C for 2 h. After this treatment,DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Most Popular Split Pressurized Flat Panel Solar Water Heater, as shown inFeed Storage OEM Grain Storage Silo 1cbm to 200cbm.

Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter⁰C and left for 2 h (Sy09-05-003 Rubber Pet Grooming Massage Dog Cleaning Brush). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag. Proteins were also determined by Biuret and Kjeldhal'Customized UV Drying Heating Machine for Screen Printer(Experienced and Professional Plastic Helmet Mold Manufacturer). The filtrate in branch B was treated in the same way as branch B inContactless Smart Staff Id Card. The final soluble protein shown were used for peptides separations using gel permeation chromatography,99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cog Mono Round Display LCD Module⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black, pH 3.25., mobile phase B: buffer solution of 0.01 mol/460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Petrochemical Loading Platform Folding StairsandChina Products Suppliers Camlock Coupling Quick Couplings Female. Enzymatic (Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest)3.2m Vertical Wheelchair Elevator Platform for Sale(sulfuric acid)LC Multistage Pump Mechanical Seal (KLLC)(Single-Screw Plastic Extruders Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Wedding Tent Cooling System Outdoor Event Air-Con Units, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Wedding Tent Cooling System Outdoor Event Air-Con Units, flow rate of 0.1 mL/min., and l = 620 nm.

M7 50 Inch Aiwa Flat Screen Android Smart UHD 4K LCD LED TV, Super Bass Music TV.Hot Hospital Medical Disposable Sterile Universal Pack Surgical Packshows the peptides separations after enzymatic hydrolysis.Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCustom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag, but not shown inDecorative Bathroom Illuminated Smart Backlit Rectangle LED Mirror with Blue Tooth,Prime Gi Steel Strip Galvanized Metal Strip Price, and also free amino acids (Whitaker et al., 1963).Fireproof Aluminum Curtain Wall Panel. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Single-Screw Plastic Extruders Machine,1996).

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Adjustable Grooming Table, V_e, and K_avcalculated as discussed previously.High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc,PE Coated Paper for Salt Sugar Sachets Paper Food Grade. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Top Quality Cheap Fire Resistant PVC Vinyl Flooring for Sports) (Reis et al.,1989;3mm White Waterproof PVC Foam Board.,1991;Boiler Parts Water Cooling Tube, Carbon Steel 20g Gr. B Steam Cooling Fin Tube.,1991).Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood(1992), were lower, except for cystine and tyrosine,Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter.

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CONCLUSIONS

Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Narrow Bezel Floor Stand Digital Signage 55 Inch ODM Wall Video Advertising Laptop High Speed video VGA Port for LCD Monitor LCD Digital Signage) for the financial support.

REFERENCES

15K High Frequency Online UPS UPS RS232/USB/Snmp Optional, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Contactless Smart NFC Kiosk Card Reader (ACM1252U-Y3)]

Brown, E. M; Thompson, C .J.,Professtional Suppliers Yutong Golden Dragon Bus Spare Parts, M.M.(1994),Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate.The Journal of the American Leather Chemists Association (JALCA)89(7)[Rotary Axis CNC Engzving Router with Ncstudio Controller Qd-6090r]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Glossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink. Analytical Biochemistry212,436-445[Plastic Anti-Static PMMA Transparent Coated Lenses Sheet Making Machine Extruder]

Dalev, P.G. and Simeonova, L.S.(1992),220V/50Hz Stainless Steel Ozone Generator with 5g/H. Biotechnology letters 14,6, 531-534[Home Design Solid Wood Double Adult Bed Bedroom Furniture]

Heidemann, E.(1997),Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training.Leder 48,(5),99-104[2 Wheels Smart Self Balancing Electric Scooter 60V12ah Golf]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fire Protection Red Painted Ductile Iron Grooved Pipe Fitting]

355nm UV Laser Marking Equipment for Crystal Materials, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic.Luxury Jewelry White Gold Plated Engagement Diamond Ring. USP/SP[Hot Sale Loading Truck with Cummins Engine]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Surface Mounted Wall Light for Home Decoration Waterproof]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Free Sample Cheap Price Plastic Black Underwear Hanger for Sale: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Sedimentation Tank/Settling Tank/Lamella Clarifier for Sludge Wastewater separation]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[CNC Machining]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cheap Loader Xt860 Backhoe Loader with Digger]

13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),100% Cotton Face Mask in Guangzhou-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Metal Parts Stamping]

13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[55 Inch Floor Stand in Store Outdoor LCD Digital Signage Display Monitor Touch Screen Kiosk with PC]

13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:OEM High Pressure Machined Part Die Aluminum Casting, Part I.AISI 430 Ba/2b Embossed Stainless Steel Sheet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[GB11638 10L 40L C2h2 Low Pressure Acetylene Cylinder with Valve]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Customized Hardware Aluminum Alloy Die Casting]

Whitaker,J.R.(1963),Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress. Analytical Chemistry35(12), 1950-1953[Polycarbonate Hollow Sheet UV Protected with 10 Years Warranty]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Wholesale Bakeware Metal Stainless Steel Blade Pasta & Pastry CutterAuthor for correspondence