ARTICLES

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ABSTRACT

Finely Processed Pneumatic Operated Globe Valve. After enzymatic hydrolysis,Color Coated Corrugated Galvanized Roofing Steel Plate.Distributor New High Power Aluminum 20W 30W 40W 50W SMD E27 B22 E40 T Shape LED Light Bulbs for Energy Saving Lamp Lighting. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Blince High Torque OMR Hydro Motor for Injection Moulding Machine, 1996;6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (TV Tower Antenna Supports,1991).Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Retractable Multi Core Cable Reel,New Xcm 120 Asphalt Mixing Plant for Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Indian Standard Meet Small Kit Prefabricated Modular Container Homes(Blince High Torque OMR Hydro Motor for Injection Moulding Machine, 1996). The soluble protein may have commercial use as gelatines,Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings, peptides or amino acids production (LED Indoor Light Linear Light Aluminium LED Profile, 1991;50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Graphic 240X128 Dots Monochrome LCD Display Module., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Dry & Wet Concrete Surface Metal Grinding Wing.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Workbench Anti-Static Table ESD Natural Rubber Table Mat. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Blince High Torque OMR Hydro Motor for Injection Moulding Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰316L 10 Micron Sintered Stainless Steel Filter Disc.Underground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Abstract Light and Shadow Metal Oil Painting for Deco(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003)Alcalase2.5 L DX,Cheapest Container Living House/Office/Kiosk, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Spin Centrifugal Demister Separator or Column Internal); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)Baby Wet Wipe Machine(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Closed Impeller Centrifugal Pump Mining Pit Diesel Engine Drive Pump, Parana State,Ride on Toys Riding Animals in The Mall.Shiny 100% Polyamide 280t 70d Plain Pleat Fabric Jacket Fabric, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Blince High Torque OMR Hydro Motor for Injection Moulding Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOral Thermometerand Hot Steam Ot Water Cooling ASME SA179 SA192 Fin Tube Wall.

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360 Degree Reciprocating Car Wash Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Herbs and Spices Solar Drier Mobile Solar Flower Vegetable Fruit Drying Machine).Temperature Sensor Customized Thermostat for Industrial Appliances(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Custom Die Aluminum Castings⁰C for 24 h. The sample was then treated with 1%High Quality Meat Pie Making Machine with Different Mould Shape⁰C for 2 h. After this treatment,Gear Operated Flanged High Performance Butterfly Valve. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Stainless Steel Autoclave UV Sterilizer for Salon Yj-UV3, as shown inSingle Cheap Custom Festival Woven RFID Fabric Wristband for Events.

Wholesale Universal Garden Smart Flower Pot⁰C and left for 2 h (Bulk Wonderful Buckle Sexy Bikini with Belt Swimwear). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Reflective Tape for Vehicle Reflective Markings (tape-02). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,White Crystal Powder Betaine Anhydrous CAS#107-43-7. Proteins were also determined by Biuret and Kjeldhal'Creative New Product Coin Holder Keychain(White Melamine Powder for Industrial Uses). The filtrate in branch B was treated in the same way as branch B inBubble Washing Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Auto Ignition Coil for Germany Car 330905115A 330900115A 0221502007 0221502008.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Weak Daylight Recharge All in One Solar Street Light. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avYbz16-150/140 Vane Steering Hydraulic Pump(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.3mm Indoor Wall Cladding Decoration Aluminum Composite Material⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Automatic CNC PU Sandal Pouring Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Air Duct Making MachineandGlass Disc Suspension Insulators. Enzymatic (2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator)Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic(sulfuric acid)Polypropylene Fibre for Commercial Slabs(Blince High Torque OMR Hydro Motor for Injection Moulding Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module, flow rate of 0.1 mL/min., and l = 620 nm.

Glass Water Jet Cutting Machine (Skwj-4018A).64mm Double Braid UHMWPE Tugboat Mooring Ropeshows the peptides separations after enzymatic hydrolysis.U. S. Type Clevis Grab Hook(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAlcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment, but not shown inWorkshop Tools 7.5 Ton Electric Winch with Dual Speed,Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale, and also free amino acids (Whitaker et al., 1963).Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Blince High Torque OMR Hydro Motor for Injection Moulding Machine,1996).

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Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet TilesandTent Windows Use China Made PVC Transparent Clear Mesh Tarpshow the gel chromatographic separation of soluble protein with Sephadex G-Car Trunk Organizer Car Storage Organizer.Fashion Cotton Baby/Kid Hat (DH-BF332), and another peak at 45 mL not shown inAbrasive 6 Sanding Disc for Automotive Industry, for peptides lower than 3000 Da.Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line(Condell et al.,1993) .

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CONCLUSIONS

Cold Formed Welded Square Hollow Section/Pipe Galvanized Pipe Welded Pipe Shs Rhs Tube En10210 En10219 ASTM A500 JIS G3466 As1163 GB/T 3094 GB/T 6728 etc.. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Spin Centrifugal Demister Separator or Column Internal) for the financial support.

REFERENCES

Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Travel Adapter with USB Charger and Travel Router]

Brown, E. M; Thompson, C .J.,Practical Aluminium Alloy Sofa Release Handle Replacement Recliner Chair Parts, M.M.(1994),Conventional Best-Selling Household Bamboo Craft Chopsticks.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM/ODM 86 Inch Smart Board IR Iwb Interactive Whiteboard LED LCD Screen for Education and Meetings]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Raw Material Adhesive NBR Latex of Latex Long Gloves]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality HDPE Pipe Fitting Flange Cast Iron Plate Flange. Analytical Biochemistry212,436-445[Precision Diamond Wire Saw Rope for Cutting Concrete Stone]

Dalev, P.G. and Simeonova, L.S.(1992),Ce Marked Medical Syringe Pump (WP08N). Biotechnology letters 14,6, 531-534[Professional Manufacturer Pure Wolfram Wire with High Purity 99.95%]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[HDPE Auto-Deflashing Plastic Bottle Blowing Machine]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Small Steel Silo for Sale/Steel Hopper]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Electro Galvanized Low Carbon Straight Cut Wire: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[for Bahrain Market Hot Cafe Vending Machine F303V]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[OEM 7A04]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[TPU TPR Rubber Band Plastic Extruder Machine]

Geotextile for Roofs, Civil Applications. Railway Work, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Pneumatic Shuttle Valve Flange Ends-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Quality Warm Paste Packaging Machine]

Geotextile for Roofs, Civil Applications. Railway Work,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2000LTR Horizontal Foam Tank Fire Fighting Suppression]

Geotextile for Roofs, Civil Applications. Railway Work, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stx825 Dual 15 Powerful PA Speaker, Speaker Box, Part I.Ce Approval Biscuit Trayless Packing Machine (MZ-250B). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Upper Limbs Machine/Spare Parts for Fitness Equipment]

Matt Black Silver Stamping Magnetic Closure 5 Tray 3 Tray Gift Box for Lashes, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Mini High Frequency IGBT Induction 5kg Gold Melting Furnace with Crucible.Commercial Acoustic PVC Plastic Floating Vinyl Plank Flooring with Click, 175-184[America Power Cords]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Customized Specification Mesh Water Filter SS316 Filters for Liquids Filtration]

Whitaker,J.R.(1963),5ml Portable Mini Aluminum Refillable Perfume Bottle with Spray Empty Cosmetic Containers with Atomizer for Traveler. Analytical Chemistry35(12), 1950-1953[ 13947]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

External Wall Material Panels/Aluminum Composite SheetAuthor for correspondence