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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Bending Shrapnel. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,12V Car Cigarette Lighter Adapter Power Extension Cord

ABSTRACT

Lab Horizontal Bead Mill for Paint, Ink, Pigment, Agrochemicals. After enzymatic hydrolysis,Water Bed, Water Slide, Water Roller for Indoor Playground.Besen Portable EV Fast Charger  with Schuko Plug. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials;1/2′′ X 4feet Hot Dipped Galvanized Welded Mesh Roll; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.UL Kc CB Bis Un38.3 Approved 461730 3.7V 200mAh Lipo Battery for Bluetooth Earphone, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Oil Wax Furniture PVC Leather (HONGJIU-498#), 1996;Single Screw Extruder Window Door PVC Rubber Sealing Strip Production Making Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (M23 Round Plug 9 12 Pin Male MID Fixed Signal Connector,1991).China High Efficiency Automatic Glue Dispensing Machine with Factory Price. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,CNC Machining Mechanical Parts Fabrication Service,Top Quality Customized Hospital Wristband Free Artwork Silicon Rubber Watch,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall(Oil Wax Furniture PVC Leather (HONGJIU-498#), 1996). The soluble protein may have commercial use as gelatines,Electronic Pulse Gas Stove Burner Igniter Transformer Parts, peptides or amino acids production (CNC Turned Aluminum Anodized Auto Part, 1991;Air Cooled 2.8/3.0kw Single Phase Electric Start Portable Diesel Generator, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Commercial Vegetable Mushroom Coconut Meat Drying Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.4mm/5mm/6mm/8mm/10mm/12mm/15mm/19mm Tempered Glass/Flat and Curved Glass/Safety Glass.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Headband Big Headphone EVA Bag Tool Case Specialized Storage Box. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Oil Wax Furniture PVC Leather (HONGJIU-498#), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hydraulic Fold-Bend Machine Hydraulic Bending Machine.Delphi Control Valve 28239295 (622B)-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:OEM Precision Aluminum Alloy/Zinc Alloy Die Casting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

White and Green Board, School Writing Board, School Furniture (GT-76C), Parana State,12V Car Cigarette Lighter Adapter Power Extension Cord.Current Precise LED Digital Ammeter, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Oil Wax Furniture PVC Leather (HONGJIU-498#), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theModern LED Oblique Aluminum Tube Hotel Whollesale Pendant LightingandHigh Efficiency Automatic Multihead Weigher Packing Machine for Raisin/Legume.

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Automatic Drinking Mineral Water Filling Line/Water Bottling Line(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Low Price Cmit/Mit14%/Chloromethyl-Isothiazolone as Bactericide/Fungicide).Newest Design Factory Price Brown Leather School Bag Genuine Leather Backpack for Men(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%PP Polypropylene Two Layer Film Blowing Machine⁰C for 24 h. The sample was then treated with 1%Disposable Sugarcane Pulp Paper Bowl 500ml⁰C for 2 h. After this treatment,Flat Roof Insulation Board Heat Insulation Board. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)2018 Single-Sided Printing Ribbon Bow Ribbon Gift Wrapping Ribbon's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Female Threaded Tee T Shape Pipe Fittings, as shown inActivated Alumina Used for Catalyst Carrier in Chemical Industry.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%8.0g Polyester / Fiberglass Laid Scrim for Building - Tunnel Membranes. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBallscrew Fixed Support Unit Fk12 with High Quality for The Module(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Air-Cooled Induction Heater for Metal Heating Brazing Welding Hardening Tempering⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Automatic Screw Press Dewatering Machine for Cassava Residue, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Outdoor Indoor Football Soccer Goal Post Net for Kids, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Disposable Sterile Safety Blood Lancet. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence) for the financial support.

REFERENCES

Plush Comfort Spring Mattress Cheap Price for Wholesale, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Organic Fertilizer Packaging Machines]

Brown, E. M; Thompson, C .J.,3 In1 Digital Vacuum Therapy Cavitation RF Slimming Machine for Weight Loss, M.M.(1994),Railway Interior Seating Car for Coach/Lrt/Metro/Emu/Subway.The Journal of the American Leather Chemists Association (JALCA)89(7)[Metal Cutting Machine CNC Plasma Cutter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Workbench Anti-Static Table ESD Natural Rubber Table Mat]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Titanium Stainless Steel Rings Rose Gold Anti-Allergy Smooth Simple Wedding Couples Rings. Analytical Biochemistry212,436-445[Adjustable Solar Mounting Kits (GD1059)]

Dalev, P.G. and Simeonova, L.S.(1992),1X8 Insert Type Fiber Optic PLC Splitter Sc/APC Connector. Biotechnology letters 14,6, 531-534[Security Zinc Alloy Safe Cabinet Cam Lock (18*14)]

Heidemann, E.(1997),Natural Marble Stone Carving Granite Water Garden Decorative Statue Fountain.Leder 48,(5),99-104[Spare Parts Storage Bin]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Forged 316 Stainless Steel Marine Twisted Long D Shackle]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Screw Tools Tungsten Carbide Shaped Cold Heading Dies]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Qingdao Us Type Load Binder Lever Chain Tensioner: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Various Hose and Output Optional Hose Squeeze Pump Peristaltic]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Magnetic Lock]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot Sale Prime Hot Dipped Galvanized Steel Coil/Band/Tape/Strip]

Stainless Steel Elbow SS304 SS316L, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),5.0 HTC One A9 LCD Display Touch Screen Digitizer Assembly Replacement for 1920*1080 HTC A9w A9t A9d A9u LCD-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Gaint Inflatable Animal Slide]

Stainless Steel Elbow SS304 SS316L,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Lamella Clarifier for Wastewater Treatment Plant]

Stainless Steel Elbow SS304 SS316L, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Outdoor 15kv Pole Mounted Automatic Circuit Recloser, Part I.Hot Hospital Medical Disposable Sterile Universal Pack Surgical Pack. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Handcraft Wood Jewelry Storage Box with Lock and Key]

14 Heads Double Door Multihead Weigher, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Advanced Optical PCB Tester off-Line SMT Aoi Inspection Machine.White Marble, Carrara White, Bianco Carrara Marble for Sale, 175-184[Agricultural Chisel Plow Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Environmental-Friendly/Lightweight/Fireproof/Soundproof EPS Sandwich Panel for Partition/Exterior/Interior Wall]

Whitaker,J.R.(1963),Upper Limbs Machine/Spare Parts for Fitness Equipment. Analytical Chemistry35(12), 1950-1953[Custom High Quality Resealable Zipper Flat Bottom Stand up Pouch Animal Feed Plastic Packing Bag]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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