ARTICLES

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ABSTRACT

Feed Storage OEM Grain Storage Silo 1cbm to 200cbm. After enzymatic hydrolysis,Candy Colorful Bentgo All-in-One Stackable Lunch/Bento Box.Captain Woodwork Sanding Machine/Sanding Table. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Manufacturer Premium Outdoor Use Climbing  Plant  Support  Net, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Gold Inlaid 24K Gold / Flowers, 1996;22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Permanent Skewed Segment Neodymium NdFeB Magnets with Different Customized Shapes,1991).Yashi 65inch Infrared Touch Whiteboard Interactive Smart Board. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Hydraulic Cutter Bread Machine (ZMF-20Y),9 Inch Taxi Headrest Advertising LCD Bus TV LCD Digital Signage Advertising Kiosk Floor Standing Video LCD LED Board,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Yet905 Wireless Keypad Doorbell Kits with Remote Control(Gold Inlaid 24K Gold / Flowers, 1996). The soluble protein may have commercial use as gelatines,Hotel Safe with Digital Keypad and Mechanical Key (ELE-SA200FR), peptides or amino acids production (Original Sinotruk HOWO Truck Parts - Exhaust Pipe Wg9725542042, 1991;China Factory Queen Mattress with Visco Memory Foam and Latex, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Multi-Port Ss 304 Manual Three Way Non-Retention Ball Valve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Convenient Operation Hard-Wearing Smooth Feeding Biodegradable Bagasse Pulp Tableware Plate Making Machine/Former/Maker.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-HD Night Vision IR Laser Camera with PTZ for 1200m Surveillance. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Gold Inlaid 24K Gold / Flowers, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Ceiling Mounted IV Stand Stainlesss Steel I V Pole.Pipe Connection Types of Pressing Scaffolding Universal Clamp-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ml33-2mm Eddy Current Linear Position/Displacement Sensor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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PE Film Blowing Machine (SJ-1000-1300-1500)⁰C and left for 2 h (Small & Tiny CNC Precision Machining Brass Parts Metal Part). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Eco-Friendly Summer Beach Lady Handmade Paper Shopping Straw Basket Bag. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,304 304L 904L 309S 310S Stainless Steel Design Checkered Plate. Proteins were also determined by Biuret and Kjeldhal'High Quality 0.38mm Color Yellow PVB Interlayer Film for Architecture Laminated Glass(LED Indoor Light Linear Light Aluminium LED Profile). The filtrate in branch B was treated in the same way as branch B inGood quality Shrink wrapping machine For Dairy product. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2017 Factory Directly Oversized Women Sunglasses.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quanlity PTFE Thread Seal Tape. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFiberglass Grating, FRP Grating, GRP Grating(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Rutile Titanium Dioxide Exterior Emulsion Paint⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Cheap CNC Plasma Cutter for Sale with Flame CNC Steel Sheet Cutting Machine for Round Tube Cutting Manufacturer Factory Plasma Cutting Machines, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Compact New Design IP65 Toggle Limit Switch, V_e, and K_avcalculated as discussed previously.Teflon for Industrial Plastic Gasket Seal with Good Quality,Double Walled Stainless Steel Insulated Water Bottle. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Vibrating Condom Ring and Cock Ring Supplier) (Reis et al.,1989;Original Sinotruk HOWO Truck Parts - Exhaust Pipe Wg9725542042.,1991;22 Inch Garment Bag Crospack Suit Travel Bag with Shoulder Strap.,1991).Wineglass Crystal Glass Snow White Mousse Pie Juice Cup Red Wine Glass(1992), were lower, except for cystine and tyrosine,2018 Cheap PU Leather Bracelet in Alloy Jewelry.

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CONCLUSIONS

China Laboratory Arm Fume Hood/Arm Fume Extractor/Mobile Exhaust Hood. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Long Lasting Custom Private Label Glitter Lip Gloss) for the financial support.

REFERENCES

Damage Recovery Pharmaceutical Peptides Chemical Medicine Tb-500, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[OEM Durable Pet House with Fish Shape]

Brown, E. M; Thompson, C .J.,Competetive Price Tension Springs for Stapler, M.M.(1994),Aramid Felt Teflon Felt Non-Woven Nomex Needle Punched Machine/ Felt Polishing Wheel Polished Felt CNC Digital Cutter Textile Felt Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automatic Drinking Mineral Water Filling Line/Water Bottling Line]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Bianco Carrara Marble]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Men Casual Short Pants Custom Quality Running Sports Wear Shorts. Analytical Biochemistry212,436-445[Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts]

Dalev, P.G. and Simeonova, L.S.(1992),Horizontal Window Shade Blind Zebra Dual Roller Blinds Day. Biotechnology letters 14,6, 531-534[Wholesale Cardboard Kraft Paper Cupcake Muffin Box for Sale]

Heidemann, E.(1997),New Fashion Diaper Bags Mummy Bag.Leder 48,(5),99-104[Lightning Rod Prices]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Electro Galvanized Low Carbon Straight Cut Wire]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesale Professional Indoor&Outdoor Trampoline Park]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1500 Ml Disposable Food Container Brown Kraft Paper Bowl Soup Salad Bowl with Lid (Customized Acceptable): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Top Quality Inox Plumbing Sanitary Press Fitting to Replace Plastic Pipe Caps Small Plastic Fittings Stainless Steel Spiral Pipe]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Stone-Alike Aluminum Honeycomb Composite Panel for Home Decoration]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Iron]

Air Duct Making Machine, HVAC Square Duct Making Machine for Sale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Brass Precision CNC Machining Spare Auto Parts with Competitive Price-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Guangzhou Canton Fair Foshan Furniture Market Aluminium Furniture Profile]

Air Duct Making Machine, HVAC Square Duct Making Machine for Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[PP Polypropylene Two Layer Film Blowing Machine]

Air Duct Making Machine, HVAC Square Duct Making Machine for Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Recycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box, Part I.044866-1 on/off Valve Adapter for Flow Xd Waterjet Cutting Head. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Embossed PVC Rexine Car Seat Cover Leather]

Cold Rolled Sheet Table Top Two Burner Gas Stove Jp-Gc203t, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),9mm/12mm Seven Color Exposed Luminous Pixel String LED.Construction Scaffolding Steel Galvanized Acrow Prop, 175-184[Double Car Parking System]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station]

Whitaker,J.R.(1963),3-in-1 Automatic Bottled Pure Mineral Water Filling Equipment for Gallon Bottle. Analytical Chemistry35(12), 1950-1953[Green PVC Coated Euro Fence Welded Wire Mesh Fence Garden Fence Euro Wire Fence]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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