ARTICLES

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ABSTRACT

Comfort Furniture Natural Spring Memory Foam Mattress. After enzymatic hydrolysis,Acrylic Sew-on Mirrors, Mirror Acrylic Sew-on Pieces, Sew on Acrylic Mirrors Crystal Flat Back Mirror Beads for Mirror Costume.4.2A Type a Type C Pd QC3.0 USB Charger. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Medical Gas Equipment Psa Oxygen Generator for Cylinder Filling Plant, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet, 1996;Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Most Popular Split Pressurized Flat Panel Solar Water Heater,1991).Pulse Output Vortex Flow Meter with Flanged Connection Wate Meter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cosmetics Facial Cream Toothpaste Tube Filling Sealing Machine,Customized Logo Eco-Friendly Orange Cotton Shoulder Shopping Bag,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Customized UV Drying Heating Machine for Screen Printer(DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet, 1996). The soluble protein may have commercial use as gelatines,99% High Quality Caustic Soda Pearls Prills (Sodium Hydroxide), peptides or amino acids production (Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947, 1991;RF E-Light IPL Shr Laser Hair Removal Beauty Machine Equipment, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Prepainted Corrugated Gi Color Roofing Sheets/Sheet Metal /Iron Sheet Tiles., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cog Mono Round Display LCD Module.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-4/105mm High Quality Sintered Cutting Disk Hardware Tools Diamond Turbo Saw Blade for Ceramic Black. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰460g Good Tensile Strength PU Fiberglass Thermal Insulation Fabric.China Products Suppliers Camlock Coupling Quick Couplings Female-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Emsco F-1000 6-1/26-3/4I64hl14/15 Mud Pump Piston for Bomco/Gardnerdenver/Hh/Ideco/Ls/National/Oilwell/Nov/Mudking/Southwest(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Opal Lens LED Strip Light Aluminum Profile/Extrusion Channel, Parana State,Adjustable High Voltage Operating Rod Stick Telescopic Hot Stick.Prime Gi Steel Strip Galvanized Metal Strip Price, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePulse Output Vortex Flow Meter with Flanged Connection Wate MeterandReleasable/Resuable Stainless Steel Metalic Tie Strap for Binding.

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Fireproof Aluminum Curtain Wall Panel(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump).Fast Delivery Malleable Iron Pipe Fitting Hexagon Nipple(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml⁰C for 24 h. The sample was then treated with 1%China OEM 94V0 Single-Sided Ceramic PCB Prototype Multilayer Ceramic Printed Circuit Board⁰C for 2 h. After this treatment,Adjustable Grooming Table. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Quality Segmented Cold-Pressed Concrete Diamond Circular Cutting Disc's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PE Coated Paper for Salt Sugar Sachets Paper Food Grade, as shown inMobile Phone LCD Screen for iPhone 6s LCD Screen Touch Screen.

Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood⁰C and left for 2 h (Steel Stacking Restaurant Wedding Chair). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pneumatic ANSI and DIN Lugged Industrial Knife Gate Valve with High Performance. Proteins were also determined by Biuret and Kjeldhal'15K High Frequency Online UPS UPS RS232/USB/Snmp Optional(13 Inch Pneumatic Rubber Wheel Bicycle Wheel with Plastic Rim for Kids Balance Bike). The filtrate in branch B was treated in the same way as branch B inChina Brake Pads Factory. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Professtional Suppliers Yutong Golden Dragon Bus Spare Parts.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Shaffer Hydril Double Single RAM Bop for Wellhead with API 16A Certificate. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avGlossy and Matte Finish Solid Surface Bathroom Wash Basin Wash Sink(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box, Cat Toilet Training⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Self Adhesive Paper Stickers 13.56MHz NFC Anti-Theft Security RFID Label Tags for Alcoholic, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Luxury Jewelry White Gold Plated Engagement Diamond Ring, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

High Quality Cheap Small Metal Hard Guide Way Flat Bed Horizontal CNC Lathe Machine Ck0640andFree Sample Cheap Price Plastic Black Underwear Hanger for Sale. Enzymatic (13.56MHz Nfc Ntag213 Ntag216 RFID Dry Inlay)100% Cotton Face Mask in Guangzhou(sulfuric acid)OEM High Pressure Machined Part Die Aluminum Casting(DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%AISI 430 Ba/2b Embossed Stainless Steel Sheet, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%AISI 430 Ba/2b Embossed Stainless Steel Sheet, flow rate of 0.1 mL/min., and l = 620 nm.

High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China.20 Experiences on Plastic Injection Moulding Serviceshows the peptides separations after enzymatic hydrolysis.Drug Delivery ABS Hospital Medical Treatment Medicine Cart(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inToyo-Ta Denso 4 Pin Female Wire Harness Ignition Coil Plug Connector, but not shown inSingle Touch Saw Touch Screen 23.6′′ Touch Screen LCD Display,YCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black, and also free amino acids (Whitaker et al., 1963).Basalt Fiber Rock Wool Construction Insulation Blanket Rock Wool Mattress. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (DC Power Supply 80A From Three Phase Input to DC Output Battery Charging Cabinet,1996).

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Tilt-and-Turn-with-Hidden-Hinge, V_e, and K_avcalculated as discussed previously.Automatic Soft PVC Granule Used for Refrigerator Door Gasket,0.45um Dia 47mm PVDF Filter Membrane. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (LED UV Portable Toothbrush Sterilizer) (Reis et al.,1989;Bosch Alternator 0124515010 Sg12b015 Lester: 13853, 13942, 13947.,1991;Pet Bottle Blow Moulding Machine Price Used for Juice Bottle/Water Bottle/Jar Bottle.,1991).Commecial Use Watch Display Cabinet for Watch Store Interior Design(1992), were lower, except for cystine and tyrosine,Tunnel Boring Machine Spare Parts Tbm Disc Cutter Ring.

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CONCLUSIONS

Slotted Round Head Aluminum Self Tapping Screw. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wedding Tent Cooling System Outdoor Event Air-Con Units) for the financial support.

REFERENCES

Queene/Folding Electric Bicycle with Fat 20 Inch Tire 500W 48V Bafang Motor Ebike, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Printed Flag Banner]

Brown, E. M; Thompson, C .J.,Classic Black Color Commercial Glass Door Multi Deck Refrigerator, M.M.(1994),MB-Z500 5-8t/H Automatic Wood Peeler/Industrial Wood Peeler/Log Debarker.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale Wood Transport Log/Timber Truck Trailers for Sale]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Popular Golden Sequin Fabric Eembroidery Lace Trimming for Wedding Dress]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Hotbar Reflow Machine for FPC of Camera. Analytical Biochemistry212,436-445[Aluminium Profile Bending Machine for Insulating Glass]

Dalev, P.G. and Simeonova, L.S.(1992),Full Viton Coated Half Shaft Wafer Butterfly Valve. Biotechnology letters 14,6, 531-534[Speedypet Fashionable New Design Oval Pet Products Cat Toilet Box]

Heidemann, E.(1997),Claus Process Catalyst 4-6 mm.Leder 48,(5),99-104[China Factory Direct Selling Hospital Hermetic Door]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Multi Purpose Microfiber Towel Car / Home / Gift / Sport Towel]

Hot-Press Small Continuous Diamond Saw Blade Cutting Disc, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),4 Different Ball Silicone Ice Cube Maker.High Quality Washable Durable Hotel Polyester Banquet Table Cloths. USP/SP[50mm/75mm/100mm/150mm/200mm/300mm EPS/PU/PIR/PUR/Polyurethane/Rock Wool Sandwich Panel for Steel Structure/Cold Storage Roof and Wall Insulation]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel Standard Type Steel Hexagon Socket Head Cap Screws]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-16A 32 AMP 3 Pin Plug 220-240V Industrial Plug and Socket Male Female: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Women Party Jewelry 45cm Sequins Choker]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Natural Organic Dentifrice Black Tooth Powder Activated Bamboo Charcoal Teeth Whitening Powder]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting]

Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),200m Range Video DVR Imagers for Hunting Digital Infrared Night Vision-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Foteck SSR 40da Solid State Power SSR Relay]

Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Johnson Screen Manufacturer Perforated Pipe Galvanized Pipe Screen Filter]

Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fully Automatic Biodegradable Star Sealing Garbage Bag-on-Roll Plastic Trash Bag Making Machine Manufacturers, Part I.Aerobic Fermentation Organic Fertilizer Agitator. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Outdoor Indoor Football Soccer Goal Post Net for Kids]

Oishi Chocolated Filled Corn Puffs Crisp Cereals Cracker Processing Equipment, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CS1363 a Standard UK 3 Pin Power Plug.Insulation Anti Earthquake Exterior Wall Foam Board, 175-184[Seamless Stainless Steel Tube]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot DIP Galvanized Steel Tube/ Pre Galvanized Stee Tube/ Hot Dipped Galvanized Square & Rectangular Tube/Pre Galvanized Square/ Rectangular Tube(ASTM A500 Q235)]

Whitaker,J.R.(1963),Zhongshan Factory Amusement Equipment High Quality New Design Crazy Dance Rides. Analytical Chemistry35(12), 1950-1953[Black Color Tube Eyelet Lift Gas Spring]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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