ARTICLES

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ABSTRACT

Carbon Steel Round/Square Pipe Tube Production Line. After enzymatic hydrolysis,Green PVC Coated Front Yard Double Wire Mesh Fence Panel.Cable Length 11 Gun Safety Padlock. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Women′s Seamless Strapless Bra, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet, 1996;300 Degree Automatic Hot Pressing Hot Press with Mould Size 200X200mm., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Silent Diesel Generator Powered by Perkins Engine 125kVA (YMP-100),1991).(SANLI) 25m3/H Concrete Machinery Automatic Elevator Bucket Mini Concrete Mixing Station. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,off Grid Solar Inverter Household Power Frequency PV Inverter for off-Grid Solar System 3kw 5kw 10kw 20kw 30kw,Brown Cake Box Party Cupcake Pastry Cookies Packing Corrugated Paper Boxes,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Modern LED Oblique Aluminum Tube Hotel Whollesale Pendant Lighting(Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet, 1996). The soluble protein may have commercial use as gelatines,Fiber Optic Terminal Equipment 1ge Epon ONU with WiFi, peptides or amino acids production (Embossed Wood Grain Decorative PVC Sheet for PS MDF PVC Metal, 1991;20 Experiences on Plastic Injection Moulding Service, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wind Protection Screen. Wind Dust Netting, Wind Dust Net., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.24K Gold Rolling Paper Shine Papers Cigarette Paper.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Two - Way Double Row Thrust Tapered Roller Bearings. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Leisure Popular Hotel Waiting Office Leather Sofa with Wooden Armrest.Ride on Toys Riding Animals in The Mall-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Finely Processed Pneumatic Operated Globe Valve(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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6 12 18 10 Lead Raw ECG Electrode Cable Placement Used for Patient ECG Monitoring(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Forged Steel NPT Threaded High Pressure Pipe Fittings Hexagon Nipple).TV Tower Antenna Supports(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Diamond Silver Ring Silver Plated Zircon Butterfly Shape Women Ring⁰C for 24 h. The sample was then treated with 1%Retractable Multi Core Cable Reel⁰C for 2 h. After this treatment,New Xcm 120 Asphalt Mixing Plant for Sale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Indian Standard Meet Small Kit Prefabricated Modular Container Homes's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggings, as shown inYCOM 1in 2 out HDMI Splitter 4K X 2K for 3D Black.

LED Indoor Light Linear Light Aluminium LED Profile⁰C and left for 2 h (High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.50g 100g Small Sachet Vegetable Seed Sunflower Seeds Packing Machine Price. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Graphic 240X128 Dots Monochrome LCD Display Module. Proteins were also determined by Biuret and Kjeldhal'Dry & Wet Concrete Surface Metal Grinding Wing(Optically Colorshift Chrome Rainbow Chameleon Pigment for Nail Beauty). The filtrate in branch B was treated in the same way as branch B inPaper Storage Cardboard Drawer Baby Gift Box. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Workbench Anti-Static Table ESD Natural Rubber Table Mat.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%316L 10 Micron Sintered Stainless Steel Filter Disc. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avUnderground Mining Material Handling Belt Conveyor Trough Idler Roller Support/Frame/Bracket(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Silo Machine Equipment for Chicken Farm Feed Use (JF-A-L003)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Cheapest Container Living House/Office/Kiosk, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Spin Centrifugal Demister Separator or Column Internal, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Custom Die Aluminum Castings.Factory Direct Selling High Waist Seamless Sportswear Women Workout Gym Fitness Leggingsshows the peptides separations after enzymatic hydrolysis.High Quality Meat Pie Making Machine with Different Mould Shape(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inCummins Generator Set Diesel Engine Spare Parts Fuel Pump Support 4991549, but not shown in2019 Kiet Hydraulic Pipe Bender Machine,Gear Operated Flanged High Performance Butterfly Valve, and also free amino acids (Whitaker et al., 1963).China Supplier Sinotruk HOWO 6X4 Trailer Head Tractor Truck. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Living Room Furniture 2 Seat Purple Velvet Back Sofa with Metal Feet,1996).

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CONCLUSIONS

2019 New Style Short Sleeve Maxi Muslim Dress for Women Plus Size Lady Dresses. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms) for the financial support.

REFERENCES

3mm Indoor Wall Cladding Decoration Aluminum Composite Material, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Multifunction COB LED Mini Pen COB Work Flashlight COB Square]

Brown, E. M; Thompson, C .J.,240*375*25mm Rectangle Galvanized Steel Access Panel for Air Duct Maintenance Cleaning, M.M.(1994),Automatic CNC PU Sandal Pouring Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Plastic HDPE Pipe Extrusion Making Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Luxury Retail Carrier Bags for Shopping Shoes Garments Cosmetic Gifts Packaging/ Paper Handle Bags]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Glass Disc Suspension Insulators. Analytical Biochemistry212,436-445[Dual Function Write Ball Pen Aluminum Stylus for iPhone Xs X Se]

Dalev, P.G. and Simeonova, L.S.(1992),2 Kw Steam Turbine Generator Stainsteel Steel Steam Generator. Biotechnology letters 14,6, 531-534[Wicker Flower Baskets for Planter]

Heidemann, E.(1997),Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic.Leder 48,(5),99-104[Thick Suiting Uniform Fabric]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Industrial Type Plasma Cutter Machine CNC Metal Plasma Cutting Machine]

High Quality Low Volume Production Aluminum CNC Machining Auto Parts, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Polypropylene Fibre for Commercial Slabs.Compatible 1000base-Bx Bidi SFP 1490nm-Tx/1310nm-Rx 20km Dom Transceiver Module. USP/SP[Indian Standard Meet Small Kit Prefabricated Modular Container Homes]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[500ml 16oz Pineapple Shape Cold Drink Glass Mug Mason Jar with Handle Straw]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Glass Water Jet Cutting Machine (Skwj-4018A): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Quality 0.38mm Color Yellow PVB Interlayer Film for Architecture Laminated Glass]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Popular Hot Sale Chinese Approved Tracked 6.5HP Gas Power Mini Dumper]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[OEM Aluminum Auto Spare High Precision Metal CNC Machinery Mold Parts]

U. S. Type Clevis Grab Hook, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Construction Machinery Chinese 3500kg Tws75 Wheel Loader Digger Skid Steer Loader with Best Quality and Best After-Sales Service for Sale-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Auto Defrost Supermarket Island Freezer Merchandiser with Integral Compressor]

U. S. Type Clevis Grab Hook,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Efficient Online Hot Glue Dispensing Machine for Electronic Products]

U. S. Type Clevis Grab Hook, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter, Part I.Car Trunk Organizer Car Storage Organizer. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Perfume Cosmetic Empty Eye Cream Lotion Oil Press Dropper Bottles Wholesale 10ml 15ml]

Fashion Cotton Baby/Kid Hat (DH-BF332), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Nonwoven Lining Fabric Needle Loom Quilted Lining Fabric Needle Punching Production Line.OEM Round Stainless Steel Fruit Plate Tray, 175-184[Wheel Processing Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Washable Durable Hotel Polyester Banquet Table Cloths]

Whitaker,J.R.(1963),Factory Supply Industry Grade Sodium Gluconate. Analytical Chemistry35(12), 1950-1953[Rope Making Machine for PP Danline Hawser Marine Mooring Rope Braiding Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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