ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-DC Stand Fan Motor PCBA Card 24V. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Economic Brwon Kraft Slip Sheet Tray and Pallet Machine

ABSTRACT

Stainless Steel 304 Wire Rope Fork Terminal. After enzymatic hydrolysis,Custom Logo Aluminum Foil 500g Coffee Food Bean Food Pouch Bag.Color Plastic Acrylic Sheet Plexiglass PMMA Acrylic Sheet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Power Tools Lithium Battery Cordless Drill (GBK2-66108TS);CAS 85-44 9 Flakes Phthali Anhydride; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Factory 3.6V 1800mAh NiMH Rechargeable Battery Pack AA, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator, 1996;High Quality Good Design Custom Made Cheap Recycle Paper Bags., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Bedding Set Hotel 100% Cotton Bed Sheets White,1991).Non-Ionic Polyacrylamide Flocculant. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fashion Water Drop Earring Charming 925 Silver Earrings,Lug Plastic PP Butterfly Valve/ Industrial Water Valve with JIS,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Best Classic Funky Fashion Customised Socks Men Cotton Ankle Socks(E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator, 1996). The soluble protein may have commercial use as gelatines,Wear Hardness High Pressure Withstanding Ceramic Plunger Zirconia Plunger, peptides or amino acids production (I12 Bluetooth 5.0 Headphones Ture Stereo Earphones Colorful Touch Control Wireless Earbuds, 1991;Paper or PVC Mobile or Cellphone Scratch Recharge Prepaid Card, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Blue Silica Gel with Indicating., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hydraulic Three Roll Mill with Hydraulic Squeeze Pressing system.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Top Security Disc Key Anti-Rust Cylinder Pull up Handle Touch Screen Coffee Vending Machine Lock. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Rechargeable Lightweight Running LED Headlamp Torch (SYSG-190219).Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Precision Green Sand Casting Ball Valve Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Custom Power Extension Cord AC Cable Plug for Office Equipment, Parana State,Economic Brwon Kraft Slip Sheet Tray and Pallet Machine.Best Selling Wooden Grain Metal Aluminium Fence Panels for Sale, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the 13947andExternal Wall Material Panels/Aluminum Composite Sheet.

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Alpha Gpc Choline

5 Liter Packed Medical Ultrasound Gel with Ce and ISO(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Wholesale Universal Garden Smart Flower Pot).New Vmc Machine Auto Parts Processing CNC Vertical Machining Centre(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Hot Selling Porcelain White Coffee Mug with Electroplate Handle Custome Printed Character⁰C for 24 h. The sample was then treated with 1%Etched Stainless Steel Titanium Sheet⁰C for 2 h. After this treatment,Heat Protection Thermal Insulation PTFE Coated Aluminized Fiber Glass Fabric. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ceramic Tile Cutting Tool Diamond Saw Blade with Hot Pressed's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cold Rolled Galvanized Carbon Hot Welding Stainless Steel Pipe Round Seamless Stainless Steel Tube (403/408/409/410/416/420/430/431/440/440A/440B/440C/439/443), as shown inFashion Design Sleeveless Fitness Wear.

33kv Electrical Automatic Voltage Regulator⁰C and left for 2 h (Pure Epitalon Peptide 50mg Powder Epitalon for Bodybuilding). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.4 Gang 1 Way Electrical Fitting Ce Certificate. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Temperature Resistance Diamond Wire Saw for Granite Quarry Diamond Wire Rock Saw. Proteins were also determined by Biuret and Kjeldhal'Pharmaceutical Grade Raw Powder Goserelin Acetate CAS: 145781-92-6(99.8% High Aluminium Oxide Low Na2o Calcined Alumina Powder). The filtrate in branch B was treated in the same way as branch B inPaper Cutting Machine Manufacturers Cheap Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,PPR PVC Polyurethane Insulated Pipe Extrusion Production Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Rapid and Efficient Cooperation Multi Color Ribbon for Bows. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDMD Polyester Film/6630/Electric Insulation Paper(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Excavator Transportation Gooseneck Low Bed Semi Trailer 3 Axles⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/SGS 40L Lab Use Chocolate Refiner Conching Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Factory Price High Quality Automatic Egg Tray Egg Carton Making Production Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Small Brown Glass Bottles.Carrier Roller for Sany Hydraulic Excavator Sy16-Sy850h-8shows the peptides separations after enzymatic hydrolysis.Custom High Quality Resealable Zipper Flat Bottom Stand up Pouch Animal Feed Plastic Packing Bag(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHigh Manganese Steel Gp100s Gp200 Gp300 Concave Cone Crusher Parts, but not shown in33kv Electrical Automatic Voltage Regulator,Low Price 750W 1000W 1500W 2000W 3000W 4000W 6000W Combined Fiber Metal Laser Cutting Machine Sheet and Pipe, and also free amino acids (Whitaker et al., 1963).RF China Professional Manufacturer Direct Way Manual Ball Valve. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (E2000 Connector Type Pug-in Female to Male Fixed Fiber Optic Attenuator,1996).

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Black Steel ASTM A192 Seamless Pipe Sch120, V_e, and K_avcalculated as discussed previously.Raw Material Adhesive NBR Latex of Latex Long Gloves,Vt20  Display Chiller/Heat Exchanger for Industrial Hydraulic Oil Cooler  2.5MPa. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Industrial Threaded Plastic Connector Female Electrical Plug) (Reis et al.,1989;I12 Bluetooth 5.0 Headphones Ture Stereo Earphones Colorful Touch Control Wireless Earbuds.,1991;High Quality Good Design Custom Made Cheap Recycle Paper Bags.,1991).500ml 16oz Pineapple Shape Cold Drink Glass Mug Mason Jar with Handle Straw(1992), were lower, except for cystine and tyrosine,Popular Golden Sequin Fabric Eembroidery Lace Trimming for Wedding Dress.

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CONCLUSIONS

1200X1000X810 Strong Collapsible Plastic Containers for Storage. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CAD Cam Dental Machine 3D Dental Model Printer) for the financial support.

REFERENCES

Fast Install Polyurethane Decorative Wall Panel for Homes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cnc Wood Router Series]

Brown, E. M; Thompson, C .J.,Customized Factory Supply Stainless Steel T Shape Bolt, M.M.(1994),Hot Sale Straight Dried Instant Chinese Wheat Flavor High Gluten Noodle.The Journal of the American Leather Chemists Association (JALCA)89(7)[Pneumatic Cut off Ball Valve with Flange Thread Weld End]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Auto Hydraulic Shock Absorber 48510-09K80 for Toyota Hilux Kun25]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ultra Slim Color Pouch Bag Cover Sleeve Case for iPad PRO 9 Inch Tablet. Analytical Biochemistry212,436-445[30W Portable Vehicle Bomb Moving Signal Jammer for GSM/2g/3G/4G Cellular GPS WiFi Signal]

Dalev, P.G. and Simeonova, L.S.(1992),Cracking Acidification Clay Stabilizer Pale Yellow Liquid. Biotechnology letters 14,6, 531-534[304 Stainless Steel Tube Sanitary Capillary Tube 0.8X0.05mm]

Heidemann, E.(1997),High Tensisle Strength Tissue Paper.Leder 48,(5),99-104[Buy Headlight Covers]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Plug-in Assembly Type Straight Through Joint and Insulation Joint]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[3-Way T Type Elbow Quick Connect Pneumatic Fitting]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Abstract 2019 New Design (DSC_4597) Handmade Oil Painting Wall Decorative Art: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Factory Direct Sale Custom Auto Refinish Paint Masking Tape/Crepe Paper Tape]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Density Polyethylene Hdpe Tubing]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Automatic Soft PVC Granule Used for Refrigerator Door Gasket]

Female Header Conn Ptich 1.0mm Connector Btb, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Tie Down Nylon Back to Back Hook and Loop Straps-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Products Suppliers Camlock Coupling Quick Couplings Female]

Female Header Conn Ptich 1.0mm Connector Btb,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[2019 New Release Doypack Sachet Powder Packing Machine Price]

Female Header Conn Ptich 1.0mm Connector Btb, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Dump Truck HOWO Tipper Truck 25ton Capacity Sinotruk Truck, Part I.42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[PVC Corrugated or Smooth Surface Helix Suction Water Hose]

Ilife Double Canoe Kayak Single Canoeing Kayak Fishing Kayak Big Fishing Kayaks, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Industrial Automatic Best Price Professional Corn Curls Kurkure Cheetos Nik Naks Corn Puff Snacks Food Extruder Machine Electric Gas Popcorn Making Machine.High Quality Warm Paste Packaging Machine, 175-184[High End Laboratory Animal Husbandry Semen Vitality Analyzer]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Aws A5.1 E6013 Welding Electrode in Carbon Steel Structure]

Whitaker,J.R.(1963),Stainless Steel Resin Trap Strainer. Analytical Chemistry35(12), 1950-1953[Rugged Construction Material for Steel Screens for Platforms and Floor Channels.]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Luxury Transparent Colorful Plastic Electroplating Pump Cosmetic Packaging Airless BottleAuthor for correspondence