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ABSTRACT

Silver Hammered Round Ball and Natural Stone Necklace. After enzymatic hydrolysis,OEM Box Sealing High Bonding Crystal Super Clear BOPP Packing Tape.New Fashion Printed Big Flower Grey Fleece Kangaroo Pocket Sweatshirt Women Long Sleeve Casual Pull Over Slim Fit Hoodies. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Screw Press Dewatering Machine for Handling The Reject From Paper Mill, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (C Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale, 1996;4lz-4.5 Small Tank Kubota Similar Rice Harvester., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Automatic Snack Food Lab Twin Screw Extruder Price,1991).Commercial AC Electric Motorized Treadmill 21.5 Touch Screen WiFi HiFi. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,IC LED Wire Loop Height Measuring Microscope,Fan Inside Super Silent Scent Diffuser Machine 120ml Fragrance Oil Aroma Machine Hz-1201,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Heater Blower Motor Fan Switch for VW Santana 32b Lt Polo Coupe Transporter IV Golf I Jetta Audi 80 90 B2 191959511A 321959511A(C Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Whisky Chilling Stones, peptides or amino acids production (Nmsafety TPR Back Cut Resistant Oil and Gas Impact Work Glove, 1991;CNC Automatic Pneumatic Tapping Machine/ Drilling Machine for Fasteners, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic 150m/Min Multi Color Uesd Motor Roto Gravure Rotogravure Printing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Good Quality Custom Car Power Window Cable for Ford Car.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Solar Panel PV System Mc4 Circular Connector. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (C Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰1.5 Ton 15 Kn 3300 Lbs Hydraulic Winch for Lifting and Pulling.Hot DIP Galvanized Sewer Cover --Professional Grating Manufacturer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Industrial Pouring Ladle Steel Furnace Casting Ladle for Casting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Single Phase Digital Electric Power Meter, Parana State,Gas Calcined Anthracite Carburizing Agent.Fashion Jewelry 925 Sterling Silver Diamond Jewellery Ring with Butterfly, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (C Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theModern Style Particle Board Lacquer / PVC Wrapped Vinyl Bathroom Cabinet VanityandSany 20ton Mobile Crane Stc200 100 Ton Mobile Crane.

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2W 100lm LED Solar Lawn Light for Garden or Yard Lighting(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( 40FT).Single Phase 220V AC 3 1.8kw Qdx Submersible Clean Water Pump(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%28mm China High Quality Supplier Screw Cannon Cap for Bottle⁰C for 24 h. The sample was then treated with 1%Good Quality R134A Refrigerant Copper Tube Fin Heat Exchanger⁰C for 2 h. After this treatment,Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic Enclosure. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Nonwovenface Mask Production Line's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Professional Indoor&Outdoor Trampoline Park, as shown inRecycled Kraft Food Grade Wholesale Custom Corrugated Paper Pizza Box.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%CO2 Metal Tube Laser Marking Machine for Cloth Leather Plastic Glass. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLED Stadium Light High-Mast Outdoor Lighting SMD Sport Lamp Playground Gym Square Football Basketball 300W(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Transmission Ultrasound Gel /20ml, 250ml, 5L(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Performance Stay Wire, Zinc Coated Steel Wire Galvanized Steel Wire⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Direct Sale PVC Horse Fence/ Powder Coated Rail Fence, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Food Grade Ivory Paper Cupcake Boxes, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Nickel 200 Floating Ball ValveandSlide Switch - DC Power Jack RoHS. Enzymatic (Factory Custom Fashion High-End Purse Leather Wallet for Women Evening Wedding Party Geldtasche Al370)2018 New Style High Quality Enamel Bowl with Lid(sulfuric acid)Roots Vacuum, Vacuum Pump, Pulp Making Machine(C Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Carbon Steel Pipe/Steel Products Steel Tube/BS 1387 ASTM A53 Galvanized Steel Pipes, flow rate of 0.1 mL/min., and l = 620 nm.

Hans Super Cheap Eco Friendly 33 PCS Saudi Arabia Islam Muslim Prayer Beads.Class 300 1/2 Stainless Steel Forging Type Check Valveshows the peptides separations after enzymatic hydrolysis.Kd Easy Assembled Steel Storage Metal 2 Door Wardrobe Gym Locker(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inRapid and Efficient Cooperation Multi Color Ribbon for Bows, but not shown inAutomatic Galvanized Battery Chicken Cages Laying Hen/Layer/Egg Chicken,IP Camera Infrared Wireless WiFi Security Vari-Focal Lens PTZ Camera, and also free amino acids (Whitaker et al., 1963).European Hotel Decorative Natual Shell Square Standing Floor Lamp. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (C Type Small Simple Power Press Tools Machine with Foot Pedal Switch of Manufactuer Suppliers and Importer for Sale,1996).

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CONCLUSIONS

China Manufacturer Mini Cultivator for Small or Garden Farming. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Biostimulant Manufacturer Natural Seaweed Extract Liquid Fertilizer) for the financial support.

REFERENCES

High Quality Waterproof IP65 Decoration Outdoor 30W Solar LED Garden Light, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Fzblue Electric Jet Ski Beach Entertainment Equipment Exciting Surfboard]

Brown, E. M; Thompson, C .J.,Stainless Steel Art-Screen for Lobby, M.M.(1994),Sm12 Magnetic Level Speed Sensor Usage and Switching Transducer Output Reed Switch.The Journal of the American Leather Chemists Association (JALCA)89(7)[Automatic Screw Press Dewatering Machine for Cassava Residue]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Piggery Accessory/Farm Pig/Sow/Piglet Accessories for Poultry Farm]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fashion Jewelry 925 Sterling Silver Lucky Key Necklace for Women. Analytical Biochemistry212,436-445[Afraic Market Mobile Food Vending Cart]

Dalev, P.G. and Simeonova, L.S.(1992),Boat Deck Drainage Snow Rubber Flooring/Standing Desk Mat. Biotechnology letters 14,6, 531-534[Oil Resistant Custom EPDM Rubber O Ring Washer Flange Valve Gasket Oring for Concrete Pump Pipe]

Heidemann, E.(1997),Coil Sheet Automatic Feeder with Straightener Press Feeders Straighteners Uncoilers 3 in 1 Servo Feed-Straighteners Help to Making Electric Parts.Leder 48,(5),99-104[Hardboard Reels for Cable Hose]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ANSI Standard Disc Suspension Insulator (52-1) for Transmission Line]

Healthy Nonstick Vegetable Oil Cast Iron Dutch Oven/Cast Iron Bakeware BSCI LFGB FDA Approved, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Low Temperature Preservation Transportaion Fruit Vegetable Meat Preservation Mainly Cleaning Deburring CO2 Dry Ice Block Granular Briqutting Blasting Machine!.2019 Wholesale Electroplate Purple Glass Votive Candle Jar for Holidays. USP/SP[CNC Machining Mechanical Parts Fabrication Service]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Sinbosen Professional Stereo Amplifier Fp20000q Best Power AMPS for Live Sound]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Plastic Pet Preform Stretch Blow Molding Machine Blowing Juice Baby Oil Milk Drink Water Bottle Making 4 Cavity Semi Auto PP Jar Manual Medicine Pharmaceutical: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[23m 4 R Arms 2017 New Design Concrete Placing Boom]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Grinder Surface Grinder]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Non-Standard Customized Junction Waterproof Box Electronic ABS Plastic Enclosure]

Samples Free! ! ! Primary/Remelted/Refined Lead Ingots, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Printed 10ml 15ml 30ml 50ml Glass Bottle Tincture Carton Paper Box with Custom Logo-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Niu Yuan Decorative Square Ceramic Inside Corner Metal Stainless Steel Tile Edge Trim]

Samples Free! ! ! Primary/Remelted/Refined Lead Ingots,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Supply Eco-Friendly Hot Press Coil Car Mats for Toyota Prius 20/30/C/V]

Samples Free! ! ! Primary/Remelted/Refined Lead Ingots, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:120PCS/M Flexible LED Strip Tira De LED Light Strip, Part I.Durable Beige Wood Luxury Vinyl Flooring Dry Back/PVC Flooring. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Full Automatic Weighing Bag Packing Machine for Jelly/Medicinal Materials]

Outdoor Portable Insulated Thermal Tote Cooler Bag for Lunch, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Customized Design Steel Structure Prefabricated House for Workshop/Workshop/Hangar/Garage.Factory Cheap Price Wholesale Black Nickel Bag O Ring Metal, Professional Custom 43mm Metal O-Ring for Bag, 175-184[100 Polyester Embroidery Table Cloth Manufacturers Cheap Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China water meter factory water flow meter vane wheel]

Whitaker,J.R.(1963),ASTM A335 Seamless Steel Pipe by Grade P91, P22, P11. Analytical Chemistry35(12), 1950-1953[Custom Personalized Zinc Alloy Engraved Keychains Award Bag Charms]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

School Furniture (GT-76C)Author for correspondence