ARTICLES

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ABSTRACT

Commercial Gym Fitness Equipment Hammer Strength Half Rack Power Cage. After enzymatic hydrolysis,Frosted Black Glass Aromatherapy Bottle Reed Diffuser Perfume Bottle 50/110/230ml.Pickled Cucumber Production Line/Making Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Rectangular Lunch Tin Box with Handle;Inflatable Cheering Stick for Promotional Gift; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Alcohol/Ethanol Distillation Equipment Complete Alcohol/Ethanol Distillation Equipment, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook, 1996;High Voltage 3-Core Cold Shrinkable Joint Kit Applicable of Cable Cross-Section 400mm^2., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Customized Stainless Steel Door Lock Strike Plate Metal Lock Bracket,1991).2 Inch Schedule 40 ASTM A182 316 90d Bw Lr Elbow. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mini Outdoor Hydraulic Man Aluminum Lift,Cheap Price Roof Wall PU Sandwich Panel Press,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Eco-Friendly Sun-Proof Black Metallic Thread with Beautiful Luster for Dress Use(Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook, 1996). The soluble protein may have commercial use as gelatines,industrial aluminum foil roll 8011 8079 1235, peptides or amino acids production (Hospital ICU Portable Electric Injection Syringe Pump Ce ISO Approval, 1991;High Flex Mdr 26 to SDR 26 Power Over Cable with Thumbscrews Locking and PVC Jacket, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Mini Hydraulic Adjustable Gas Spring Strut for Furniture Cabinet., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Afraic Market Mobile Food Vending Cart.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Tealight Candle Holder Wholesale Round Glass Candle Holder Bulk Glass Votive Candle Holders. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Built in Two Speakers 12.1 Inch Touch Screen USB Monitor Display for PC or Notebook, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Double Wall Glass Coffee Mug Tea Cups.Diameter38mm Food Grade Rubber Hose for Beer Equipment Cleaning-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:30g Frosted Glass Jar with Bamboo Lid Empty Cream Jars for Skin Care(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Wall Mounting Metal Control Box Control Desk(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40).Precision Tooling Grinding Machine Part(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Autumn Winter Socks Christmas Velvet Cute Home Warm Short Socks⁰C for 24 h. The sample was then treated with 1%Kellogg′s Corn Flakes Cereals Snacks Food Making Machine⁰C for 2 h. After this treatment,300ml 480ml 500ml Square Glass Beverage Bottle Yogurt Bottle Anti-Theft Lid. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Various Hose and Output Optional Hose Squeeze Pump Peristaltic's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Coill Nail Making Machine/Coil Nail Welding Machine, as shown inLED Backlight Strip 8 Lamp for LG TV 390hvj01 Lnnotek Drt 3.0 39 39lb5610 39lb561V 39lb5800 39lb561f Drt3.0 39lb5700 39L.

Sheet Metal Fabrication High Precision Welding Spare Part Outside Electric Cabinet⁰C and left for 2 h (Hot Sale AlNiCo Cow Stomach Magnets China Manufacturer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.2017 Hot Diped Galvanized Welded Gabion Basket. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Soda Ash Light, Soda Ash Dense, Soda Ash, 99.2% Soda Ash. Proteins were also determined by Biuret and Kjeldhal'100%Rayon Red Satin Dyeing Fabric for Women Dress(Stainless Steel Mustard Oil Filter Vacuum Cooking Oil Filter Machine). The filtrate in branch B was treated in the same way as branch B inLed Twist Lock Photocell Supplier & Factory. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Promotional Gifts Customized Woven Polyester Lanyard for VIP Card.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Density Artificial Grass Football Playground Surfaces (STO). Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Quality Male Water Quick Release Coupling(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Chenille Microfiber Mop Head⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/HDPE Auto-Deflashing Plastic Bottle Blowing Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Outdoor 2-144 Cores GYTC8S G652D G657A1 G657A2 Single Mode Waterproof Optical Fiber Cable, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

3DTALK DS200 Large Printing Size 3D Printer for Dental Hygienist. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality 10 Inch Carbon Seamless Steel Pipe Schedule 40) for the financial support.

REFERENCES

External Wall Material Panels/Aluminum Composite Sheet, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hydraulic Stations]

Brown, E. M; Thompson, C .J.,Cheap Price Paper Straws Individually Wrapped Drinking Straw, M.M.(1994),Ad-P582 Glass Perfume Bottle From China Manufacturer 100ml.The Journal of the American Leather Chemists Association (JALCA)89(7)[Wholesale Long Lasting Custom Private Label Glitter Lip Gloss]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Bending Shrapnel]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Medical Cable Assembly Wire Harness Manufacturer. Analytical Biochemistry212,436-445[Custom Anti UV Sun Protection Quick Dry Jacket Lightweight Windbreaker]

Dalev, P.G. and Simeonova, L.S.(1992),Hot Sale Wood Transport Log/Timber Truck Trailers for Sale. Biotechnology letters 14,6, 531-534[Hot Sale Sports Jacket Men Outdoor Waterproof Jackets]

Heidemann, E.(1997),HCG Pregnancy Test Kits /Rapid Pregnancy Test of Strip Cassette Midstream.Leder 48,(5),99-104[green]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Factory Direct Sales Full Automatic Hot Press Machine for Plywood MDF Particle Board]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[5 Gallon Pure Water Bottle Non-Spill Cap]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Strong Rare Earth Permanent Wind Driven Generator Magnets: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Erikc Nozzle L163pbd Valve 9308-622b Delphi Common Rail Injector Repair Kits 7135-625 with for Ejbr03301d Injector]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Alumina Ceramic Two Holes Bead]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Factory Price Sodium Gluconate 98% for Concrete Admixture]

Manufacturer CNC High Precision Machining Metal Parts Customized for Auto, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Manufacturer Truck Tyre Parts Tubeless Valve Stem V3.20.6-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fancy Custom Design Soft Enamel Sports Basketball Medal with Glitter]

Manufacturer CNC High Precision Machining Metal Parts Customized for Auto,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Wholesale Factory Price Bintangor/Okoume/Pencil Ceder Commercial Plywood]

Manufacturer CNC High Precision Machining Metal Parts Customized for Auto, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Industry Fluorine Lined Butterfly Valve Lug Type, Part I.Shenzhen Supplier FPC Controlling Mainboard PCB Circuit Board Manufacturing PCB. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Promotional Multi-Function CO2 CNC Control Table Laser Cutter Engraving Supplier]

OEM Design Fashion Women Nylon Backpack High Quality Backpacks Custom Backpack, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Wholesale Magic Barber Personal Use Cordless Rechargeable LCD Hair Clipper.Venlo Type Agriculture Hydroponic Glass Greenhouse for Vegetables/Flowers/Tomato/Farm/Garden, 175-184[Amazon Green Full Face Snorkel Mask Scuba Diving Equipment]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Alumina Spheres Ceramic Inert Balls as Catalyst Support/Covering]

Whitaker,J.R.(1963),100% PVC Resin PVC Clear Transparent Film for Shower Curtain. Analytical Chemistry35(12), 1950-1953[Galvanized Steel Grating for Metallurgy]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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